Fabrication a novel ethylenediamine sulfonamide polymer resin and graphene-modified carbon paste electrodes for simultaneous determination of anti-HBV drugs entecavir and tenofovir in dosage form by differential pulse voltammetry

Adel Asfoor; Zeynep Aydoğmuş; Bahire Senkal

Fabrication a novel ethylenediamine sulfonamide polymer resin and graphene-modified carbon paste electrodes for simultaneous determination of anti-HBV drugs entecavir and tenofovir in dosage form by differential pulse voltammetry

Abstract

The combination of entecavir (ETV) and tenofovir (TEV), nucleos(t)ide analogs (NUCs), is advised as first-line treatment for people suffering from multidrug-resistant hepatitis B due to their favorable safety profile, low side-effect rate, and high genetic barrier. This study reports the fabrication of a novel cross-linked ethylenediamine sulfonamide polymer resin (EDASP) and graphene (Gr)-modified carbon paste electrodes (CPE) for the simultaneous determination of the anti-HBV drugs ETV and TEV in bulk and dosage form by differential pulse voltammetry (DPV). The electrodes were specified through cyclic voltammetry, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and the analytical parameters were optimized. The findings indicate that both drugs displayed clear oxidation peaks on the Gr-CPE and EDASP-CPE electrodes at pH 4.5 and 4.0, respectively, in the Britton-Robinson buffer. The linear dynamic range of ETV and TEV was determined between 1μM and 250μM on both working electrodes by DPV. The limit of detection (LOD) and limit of quantification (LOQ) for ETV were 0.2μM and 0.6μM, respectively, on Gr-CPE. The LOD and LOQ for TEV were 0.2μM and 0.7μM, respectively. The novel EDASP-CPE exhibited improved sensitivity with LOD and LOQ for ETV of 0.2μM and 0.5μM, respectively. The LOD and LOQ for TEV on EDASP CPE were 0.2μM and 0.7μM, respectively. The proposed method was successfully applied to the simultaneous determination of ETV and TEV in a commercial tablet dosage form, exhibiting good accuracy and precision. The results highlight the potential of the novel cross-linked ethylenediamine sulfonamide polymer resin-modified carbon paste electrode for the simultaneous determination of anti-HBV drugs in dosage form by differential pulse voltammetry.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry

Journal Section

Research Article

Authors

Adel Asfoor This is me
0000-0003-3936-7144
Türkiye

Zeynep Aydoğmuş ^*
0000-0001-7784-0129
Türkiye

Bahire Senkal
0000-0002-1616-6828
Türkiye

Publication Date

June 28, 2025

Submission Date

June 2, 2023

Acceptance Date

August 7, 2023

Published in Issue

Year 2024 Volume: 28 Number: 3

IZ

https://izlik.org/JA74ZG95UL

Cite

RIS / Bibtex

APA

Asfoor, A., Aydoğmuş, Z., & Senkal, B. (2025). Fabrication a novel ethylenediamine sulfonamide polymer resin and graphene-modified carbon paste electrodes for simultaneous determination of anti-HBV drugs entecavir and tenofovir in dosage form by differential pulse voltammetry. Journal of Research in Pharmacy, 28(3), 579-602. https://izlik.org/JA74ZG95UL

AMA

1.Asfoor A, Aydoğmuş Z, Senkal B. Fabrication a novel ethylenediamine sulfonamide polymer resin and graphene-modified carbon paste electrodes for simultaneous determination of anti-HBV drugs entecavir and tenofovir in dosage form by differential pulse voltammetry. J. Res. Pharm. 2025;28(3):579-602. https://izlik.org/JA74ZG95UL

Chicago

Asfoor, Adel, Zeynep Aydoğmuş, and Bahire Senkal. 2025. “Fabrication a Novel Ethylenediamine Sulfonamide Polymer Resin and Graphene-Modified Carbon Paste Electrodes for Simultaneous Determination of Anti-HBV Drugs Entecavir and Tenofovir in Dosage Form by Differential Pulse Voltammetry”. Journal of Research in Pharmacy 28 (3): 579-602. https://izlik.org/JA74ZG95UL.

EndNote

Asfoor A, Aydoğmuş Z, Senkal B (June 1, 2025) Fabrication a novel ethylenediamine sulfonamide polymer resin and graphene-modified carbon paste electrodes for simultaneous determination of anti-HBV drugs entecavir and tenofovir in dosage form by differential pulse voltammetry. Journal of Research in Pharmacy 28 3 579–602.

IEEE

[1]A. Asfoor, Z. Aydoğmuş, and B. Senkal, “Fabrication a novel ethylenediamine sulfonamide polymer resin and graphene-modified carbon paste electrodes for simultaneous determination of anti-HBV drugs entecavir and tenofovir in dosage form by differential pulse voltammetry”, J. Res. Pharm., vol. 28, no. 3, pp. 579–602, June 2025, [Online]. Available: https://izlik.org/JA74ZG95UL

ISNAD

Asfoor, Adel - Aydoğmuş, Zeynep - Senkal, Bahire. “Fabrication a Novel Ethylenediamine Sulfonamide Polymer Resin and Graphene-Modified Carbon Paste Electrodes for Simultaneous Determination of Anti-HBV Drugs Entecavir and Tenofovir in Dosage Form by Differential Pulse Voltammetry”. Journal of Research in Pharmacy 28/3 (June 1, 2025): 579-602. https://izlik.org/JA74ZG95UL.

JAMA

1.Asfoor A, Aydoğmuş Z, Senkal B. Fabrication a novel ethylenediamine sulfonamide polymer resin and graphene-modified carbon paste electrodes for simultaneous determination of anti-HBV drugs entecavir and tenofovir in dosage form by differential pulse voltammetry. J. Res. Pharm. 2025;28:579–602.

MLA

Asfoor, Adel, et al. “Fabrication a Novel Ethylenediamine Sulfonamide Polymer Resin and Graphene-Modified Carbon Paste Electrodes for Simultaneous Determination of Anti-HBV Drugs Entecavir and Tenofovir in Dosage Form by Differential Pulse Voltammetry”. Journal of Research in Pharmacy, vol. 28, no. 3, June 2025, pp. 579-02, https://izlik.org/JA74ZG95UL.

Vancouver

1.Adel Asfoor, Zeynep Aydoğmuş, Bahire Senkal. Fabrication a novel ethylenediamine sulfonamide polymer resin and graphene-modified carbon paste electrodes for simultaneous determination of anti-HBV drugs entecavir and tenofovir in dosage form by differential pulse voltammetry. J. Res. Pharm. [Internet]. 2025 Jun. 1;28(3):579-602. Available from: https://izlik.org/JA74ZG95UL