Effects of lesinurad on HEK-293 human kidney cells: In vitro and molecular docking evaluation

Tugce Boran; Mahmoud Abudayyak; Mohammed T. Qaoud

Effects of lesinurad on HEK-293 human kidney cells: In vitro and molecular docking evaluation

Abstract

Lesinurad is a drug used for the treatment of hyperuricemia. It has been reported that lesinurad causes renal adverse events. The molecular effects of lesinurad in the kidney cells have not been elucidated clearly. The embryonic kidney cells (HEK-293) were treated with lesinurad at various concentrations (12.5-100 µM) for 24h. The cytotoxicity, apoptotic effect, reactive oxygen species (ROS), lipid peroxidation (MDA), and total antioxidant capacity (TAC) levels were evaluated. Secretion of the inflammatory mediators was examined after lesinurad treatment. Additionally, molecular docking studies were performed for lesinurad with TNF-α and concanavalin a (ConA). Lesinurad did not induce apoptotic cell death at the tested concentrations. The ROS and MDA levels insignificantly declined, and the TAC level increased. TNF-α secretion was induced after 100 μM lesinurad treatment. Lesinurad significantly decreased the Con a-induced inflammatory mediators’ secretion. The docking studies results show a weak interaction with TNF-α but strong interaction with Con a proteins. These findings support that lesinurad-induced kidney toxicity could be related to the mechanical stress of uric acid crystals rather than the induction of inflammation by the initiation of oxidative damage.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Toxicology

Journal Section

Research Article

Authors

Tugce Boran ^*
0000-0003-4302-1947
Türkiye

Mahmoud Abudayyak This is me
Türkiye

Mohammed T. Qaoud This is me
0000-0002-9563-9493
Cyprus

Publication Date

June 28, 2025

Submission Date

April 12, 2023

Acceptance Date

September 27, 2023

Published in Issue

Year 2024 Volume: 28 Number: 3

IZ

https://izlik.org/JA74HY52MA

Cite

RIS / Bibtex

APA

Boran, T., Abudayyak, M., & Qaoud, M. T. (2025). Effects of lesinurad on HEK-293 human kidney cells: In vitro and molecular docking evaluation. Journal of Research in Pharmacy, 28(3), 708-721. https://izlik.org/JA74HY52MA

AMA

1.Boran T, Abudayyak M, Qaoud MT. Effects of lesinurad on HEK-293 human kidney cells: In vitro and molecular docking evaluation. J. Res. Pharm. 2025;28(3):708-721. https://izlik.org/JA74HY52MA

Chicago

Boran, Tugce, Mahmoud Abudayyak, and Mohammed T. Qaoud. 2025. “Effects of Lesinurad on HEK-293 Human Kidney Cells: In Vitro and Molecular Docking Evaluation”. Journal of Research in Pharmacy 28 (3): 708-21. https://izlik.org/JA74HY52MA.

EndNote

Boran T, Abudayyak M, Qaoud MT (June 1, 2025) Effects of lesinurad on HEK-293 human kidney cells: In vitro and molecular docking evaluation. Journal of Research in Pharmacy 28 3 708–721.

IEEE

[1]T. Boran, M. Abudayyak, and M. T. Qaoud, “Effects of lesinurad on HEK-293 human kidney cells: In vitro and molecular docking evaluation”, J. Res. Pharm., vol. 28, no. 3, pp. 708–721, June 2025, [Online]. Available: https://izlik.org/JA74HY52MA

ISNAD

Boran, Tugce - Abudayyak, Mahmoud - Qaoud, Mohammed T. “Effects of Lesinurad on HEK-293 Human Kidney Cells: In Vitro and Molecular Docking Evaluation”. Journal of Research in Pharmacy 28/3 (June 1, 2025): 708-721. https://izlik.org/JA74HY52MA.

JAMA

1.Boran T, Abudayyak M, Qaoud MT. Effects of lesinurad on HEK-293 human kidney cells: In vitro and molecular docking evaluation. J. Res. Pharm. 2025;28:708–721.

MLA

Boran, Tugce, et al. “Effects of Lesinurad on HEK-293 Human Kidney Cells: In Vitro and Molecular Docking Evaluation”. Journal of Research in Pharmacy, vol. 28, no. 3, June 2025, pp. 708-21, https://izlik.org/JA74HY52MA.

Vancouver

1.Tugce Boran, Mahmoud Abudayyak, Mohammed T. Qaoud. Effects of lesinurad on HEK-293 human kidney cells: In vitro and molecular docking evaluation. J. Res. Pharm. [Internet]. 2025 Jun. 1;28(3):708-21. Available from: https://izlik.org/JA74HY52MA