Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity

Esma Yanpar; Metin Yildirim; Merih Akkapulu Kaya; Ulaş Değirmenci; Serpil Könen Adigüzel; Serap Yalın; Ali Erdinç Yalın

Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity

Abstract

Alcohol consumption is a tradition in most cultures but are increasingly being arised as possessing a potential for misuse. Punicalagin is a phenolic compound that is found in forms alpha and beta in pomegranates. In this study, the protective role of punicalagin on oxidative stress, inflammation and DNA damage caused by ethanol (EtOH) in liver tissue were examined. Wistar albino rats were divided into 4 groups as control, eth, punicalagin, EtOH EtOH + punicalagin. In EtOH groups, rats were treated with EtOH (4g/kg) for 21 days, in punicalagin groups, rats were treated with punicalagin (4 mg/kg) for 21 days. In the liver tissue, superoxide dismutase (SOD), catalase (CAT) activities and malondialdehyde (MDA) and glutathione (GSH) levels, in serum AST, ALT, LDH activities and TNF-α, IL-6 levels were measured. Genotoxicity was evaluated using comet assay. Based on these experimental results, while EtOH increased ALT, AST and LDH enzyme activies and induced inflammation and oxidative stress. Punicalagin reduced IL- 6, TNF-α, MDA levels, ALT, AST, LDH enzyme activities and increased SOD, CAT activities and GSH levels. EtOH significantly increased the percentage of damaged cells (type II, III and IV) and genetic damage index compared to the other groups (control, punicalagin and EtOH +punicalagin). Punicalagin was not genotoxic compared to the control. Furthermore, punicalagin reduced the genotoxic effect, induced by EtOH, with the sharp decrease in damaged cells (from 14.00±1.22 to 2.20±1.30) and genetic damage index (from 1.20±0.05 to 0.14±0.05). Punicalagin has antioxidant, anti- inflammatory and protective role against to ethanol induced liver toxicity.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry

Journal Section

Research Article

Authors

Esma Yanpar ^* This is me
Türkiye

Metin Yildirim This is me
Türkiye

Merih Akkapulu Kaya
Türkiye

Ulaş Değirmenci
Türkiye

Serpil Könen Adigüzel This is me
Türkiye

Serap Yalın
Türkiye

Ali Erdinç Yalın
Türkiye

Publication Date

June 28, 2025

Submission Date

August 27, 2020

Acceptance Date

July 22, 2021

Published in Issue

Year 2021 Volume: 25 Number: 5

IZ

https://izlik.org/JA53XL54FC

Cite

RIS / Bibtex

APA

Yanpar, E., Yildirim, M., Akkapulu Kaya, M., Değirmenci, U., Könen Adigüzel, S., Yalın, S., & Yalın, A. E. (2025). Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity. Journal of Research in Pharmacy, 25(5), 600-608. https://izlik.org/JA53XL54FC

AMA

1.Yanpar E, Yildirim M, Akkapulu Kaya M, et al. Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity. J. Res. Pharm. 2025;25(5):600-608. https://izlik.org/JA53XL54FC

Chicago

Yanpar, Esma, Metin Yildirim, Merih Akkapulu Kaya, et al. 2025. “Possible Protective Role of Punicalagin on Oxidative Stress, Inflammation and Genotoxicity in Ethanol-Induced Liver Toxicity”. Journal of Research in Pharmacy 25 (5): 600-608. https://izlik.org/JA53XL54FC.

EndNote

Yanpar E, Yildirim M, Akkapulu Kaya M, Değirmenci U, Könen Adigüzel S, Yalın S, Yalın AE (July 1, 2025) Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity. Journal of Research in Pharmacy 25 5 600–608.

IEEE

[1]E. Yanpar et al., “Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity”, J. Res. Pharm., vol. 25, no. 5, pp. 600–608, July 2025, [Online]. Available: https://izlik.org/JA53XL54FC

ISNAD

Yanpar, Esma - Yildirim, Metin - Akkapulu Kaya, Merih - Değirmenci, Ulaş - Könen Adigüzel, Serpil - Yalın, Serap - Yalın, Ali Erdinç. “Possible Protective Role of Punicalagin on Oxidative Stress, Inflammation and Genotoxicity in Ethanol-Induced Liver Toxicity”. Journal of Research in Pharmacy 25/5 (July 1, 2025): 600-608. https://izlik.org/JA53XL54FC.

JAMA

1.Yanpar E, Yildirim M, Akkapulu Kaya M, Değirmenci U, Könen Adigüzel S, Yalın S, Yalın AE. Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity. J. Res. Pharm. 2025;25:600–608.

MLA

Yanpar, Esma, et al. “Possible Protective Role of Punicalagin on Oxidative Stress, Inflammation and Genotoxicity in Ethanol-Induced Liver Toxicity”. Journal of Research in Pharmacy, vol. 25, no. 5, July 2025, pp. 600-8, https://izlik.org/JA53XL54FC.

Vancouver

1.Esma Yanpar, Metin Yildirim, Merih Akkapulu Kaya, Ulaş Değirmenci, Serpil Könen Adigüzel, Serap Yalın, Ali Erdinç Yalın. Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity. J. Res. Pharm. [Internet]. 2025 Jul. 1;25(5):600-8. Available from: https://izlik.org/JA53XL54FC