The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method

Bediha Akmese; Nurgul Karadas Bakirhan; Tuğçe Göver

The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method

Abstract

Daptomycin is known as the first lipopeptide antibiotic shows activity against gram-positive bacteria. A fast, simple and repeatabile RP-LC method has been developed for the determination of daptomycin in mobile phase solution and human urine. This drug was exposed to various stress conditions such as hydrolytic, oxidative, thermal and photolytic and the stressed samples were detected by the proposed method. Daptomycin was well separated on a X-Terra RP-18 column using the mobile phase consisting of a mixture of acetonitrile:water (45:55 v/v) at a flow rate of 1 mL min-1. The system operates at 222 nm for daptomycin and 319 nm and tinidazole was used as internal standard under these conditions. The linearity of daptomycin was obtained between 0.50 and 12 µg mL-1 concentration range. Limits of detection was determined as 0.1176 and 0.1412 µg mL-1 and the limits of quantification was obtained as 0.3563 and 0.4277 µg mL-1 in the mobile phase and urine, respectively. Hydrochloric acid, sodium hydroxide, hydrogen peroxide affected response of daptomycin under stress degradation conditions.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry, Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Bediha Akmese This is me
Türkiye

Nurgul Karadas Bakirhan ^*
Türkiye

Tuğçe Göver
Türkiye

Publication Date

June 27, 2025

Submission Date

September 25, 2018

Acceptance Date

October 31, 2018

Published in Issue

Year 2019 Volume: 23 Number: 2

IZ

https://izlik.org/JA24LW62TA

Cite

RIS / Bibtex

APA

Akmese, B., Karadas Bakirhan, N., & Göver, T. (2025). The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method. Journal of Research in Pharmacy, 23(2), 170-176. https://izlik.org/JA24LW62TA

AMA

1.Akmese B, Karadas Bakirhan N, Göver T. The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method. J. Res. Pharm. 2025;23(2):170-176. https://izlik.org/JA24LW62TA

Chicago

Akmese, Bediha, Nurgul Karadas Bakirhan, and Tuğçe Göver. 2025. “The Development of Forced Degradation Studies for the Determination of Daptomycin in Urine at Various Conditions by Using RP-LC Method”. Journal of Research in Pharmacy 23 (2): 170-76. https://izlik.org/JA24LW62TA.

EndNote

Akmese B, Karadas Bakirhan N, Göver T (June 1, 2025) The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method. Journal of Research in Pharmacy 23 2 170–176.

IEEE

[1]B. Akmese, N. Karadas Bakirhan, and T. Göver, “The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method”, J. Res. Pharm., vol. 23, no. 2, pp. 170–176, June 2025, [Online]. Available: https://izlik.org/JA24LW62TA

ISNAD

Akmese, Bediha - Karadas Bakirhan, Nurgul - Göver, Tuğçe. “The Development of Forced Degradation Studies for the Determination of Daptomycin in Urine at Various Conditions by Using RP-LC Method”. Journal of Research in Pharmacy 23/2 (June 1, 2025): 170-176. https://izlik.org/JA24LW62TA.

JAMA

1.Akmese B, Karadas Bakirhan N, Göver T. The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method. J. Res. Pharm. 2025;23:170–176.

MLA

Akmese, Bediha, et al. “The Development of Forced Degradation Studies for the Determination of Daptomycin in Urine at Various Conditions by Using RP-LC Method”. Journal of Research in Pharmacy, vol. 23, no. 2, June 2025, pp. 170-6, https://izlik.org/JA24LW62TA.

Vancouver

1.Bediha Akmese, Nurgul Karadas Bakirhan, Tuğçe Göver. The development of forced degradation studies for the determination of daptomycin in urine at various conditions by using RP-LC method. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(2):170-6. Available from: https://izlik.org/JA24LW62TA