Investigation the effects of synthetic cannabinoid-AKB48 on DNA methylation via the regulation of cannabinoid receptor gene specific methylation in vitro

Ecem Fatma Karaman; Şemsinur Yiğiter; Sibel Özden

Investigation the effects of synthetic cannabinoid-AKB48 on DNA methylation via the regulation of cannabinoid receptor gene specific methylation in vitro

Abstract

Synthetic cannabinoids have become a significant public health problem in the world because of inexpensive, easy to obtain, difficult to detect by many screening methods, constantly new compounds are produced and marketed, and so on. Despite their effect on ∆9-tetrahydrocannabinol (THC) like properties on cannabinoid receptors, they exert a very strong effect as opposed to THC and could cause serious side effects to death. Studies in the literature on causative epigenetic mechanisms of long-term usage of drugs like cocaine, cannabis, and heroin are available however, few studies have been conducted on synthetic cannabinoids, which are very common in use. Also, recently the endocrine disrupting effects of such substances have come into prominence. We aimed to examine potential effects of AKB48, one of the important synthetic cannabinoids, on cytotoxicity, global methylation and CpG island promoter methylation of cannabinoid receptor genes (CB1 and CB2) and gene expressions of DNA methyltransferase enzymes (DNMT1 and MGMT), CB1 and CB2 genes in prostate adenocarcinoma (PC-3) cells following 24 h exposure. In MTT assay, IC50 value was calculated as 343.8 µM for AKB48 in PC-3 cells. Percentage of 5-methylcytosine (5-mC%) levels were significantly decreased by 27% at 100 μM concentration. No significant alteration was shown in the promoter methylation of CB1 gene compared to the control. These results will contribute to literature for determination of potential effects of synthetic cannabinoids on epigenetic modifications in the tissues of endocrine system.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Toxicology

Journal Section

Research Article

Authors

Ecem Fatma Karaman ^*
Türkiye

Şemsinur Yiğiter
Türkiye

Sibel Özden
Türkiye

Publication Date

June 28, 2025

Submission Date

June 30, 2021

Acceptance Date

September 4, 2021

Published in Issue

Year 2021 Volume: 25 Number: 5

IZ

https://izlik.org/JA63RU54DY

Cite

RIS / Bibtex

APA

Karaman, E. F., Yiğiter, Ş., & Özden, S. (2025). Investigation the effects of synthetic cannabinoid-AKB48 on DNA methylation via the regulation of cannabinoid receptor gene specific methylation in vitro. Journal of Research in Pharmacy, 25(5), 626-633. https://izlik.org/JA63RU54DY

AMA

1.Karaman EF, Yiğiter Ş, Özden S. Investigation the effects of synthetic cannabinoid-AKB48 on DNA methylation via the regulation of cannabinoid receptor gene specific methylation in vitro. J. Res. Pharm. 2025;25(5):626-633. https://izlik.org/JA63RU54DY

Chicago

Karaman, Ecem Fatma, Şemsinur Yiğiter, and Sibel Özden. 2025. “Investigation the Effects of Synthetic Cannabinoid-AKB48 on DNA Methylation via the Regulation of Cannabinoid Receptor Gene Specific Methylation in Vitro”. Journal of Research in Pharmacy 25 (5): 626-33. https://izlik.org/JA63RU54DY.

EndNote

Karaman EF, Yiğiter Ş, Özden S (July 1, 2025) Investigation the effects of synthetic cannabinoid-AKB48 on DNA methylation via the regulation of cannabinoid receptor gene specific methylation in vitro. Journal of Research in Pharmacy 25 5 626–633.

IEEE

[1]E. F. Karaman, Ş. Yiğiter, and S. Özden, “Investigation the effects of synthetic cannabinoid-AKB48 on DNA methylation via the regulation of cannabinoid receptor gene specific methylation in vitro”, J. Res. Pharm., vol. 25, no. 5, pp. 626–633, July 2025, [Online]. Available: https://izlik.org/JA63RU54DY

ISNAD

Karaman, Ecem Fatma - Yiğiter, Şemsinur - Özden, Sibel. “Investigation the Effects of Synthetic Cannabinoid-AKB48 on DNA Methylation via the Regulation of Cannabinoid Receptor Gene Specific Methylation in Vitro”. Journal of Research in Pharmacy 25/5 (July 1, 2025): 626-633. https://izlik.org/JA63RU54DY.

JAMA

1.Karaman EF, Yiğiter Ş, Özden S. Investigation the effects of synthetic cannabinoid-AKB48 on DNA methylation via the regulation of cannabinoid receptor gene specific methylation in vitro. J. Res. Pharm. 2025;25:626–633.

MLA

Karaman, Ecem Fatma, et al. “Investigation the Effects of Synthetic Cannabinoid-AKB48 on DNA Methylation via the Regulation of Cannabinoid Receptor Gene Specific Methylation in Vitro”. Journal of Research in Pharmacy, vol. 25, no. 5, July 2025, pp. 626-33, https://izlik.org/JA63RU54DY.

Vancouver

1.Ecem Fatma Karaman, Şemsinur Yiğiter, Sibel Özden. Investigation the effects of synthetic cannabinoid-AKB48 on DNA methylation via the regulation of cannabinoid receptor gene specific methylation in vitro. J. Res. Pharm. [Internet]. 2025 Jul. 1;25(5):626-33. Available from: https://izlik.org/JA63RU54DY