Treatment of glioblastoma by photodynamic therapy with the aid of synthesized silver nanoparticles by green chemistry from Citrus aurantium

Ömer Erdoğan; Mürüvvet Abbak; Gülen Melike Demirbolat; Mehran Aksel; Salih Paşa; Gizem Yalcin; Özge Çevik

Treatment of glioblastoma by photodynamic therapy with the aid of synthesized silver nanoparticles by green chemistry from Citrus aurantium

Abstract

Blood brain barrier is very important to provide treatment locally for the treatment of glioblastoma. The use of nanoparticles has shown promise for glioblastoma treatments in recent years. In this study, the effect of the combined treatment of silver nanoparticles (AgNPs) synthesized from Citrus aurantium with photodynamic therapy (PDT) was investigated on U87 glioblastoma cells. AgNPs were characterized by FTIR, zeta potential and SEM images. U87 cells were treated with AgNPs (10 µg/mL) and/or PDT (0.5 mJ/cm2) at 24 h. Cells antiproliferative effect, migration levels, colony formation capability, Bax and Bcl-2 protein/gene expression, and caspase-3 activity levels as apoptotic markers were measured. AgNPs size were found at 141±3 nm and 18.1±1.3 mV as zeta potential. It was found that cell proliferation, migration and Bcl-2 protein/gene levels decreased, and Bax protein/gene levels and caspase-3 activity increased via AgNPs and PDT combined treatment. As a result, nanoparticles synthesized from Citrus aurantium are eco-friendly and their size can cross the blood brain barrier. If AgNPs is used with PDT, it may be a new therapy for clinically treatment of glioblastoma in the future.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry

Journal Section

Research Article

Authors

Ömer Erdoğan ^*
Türkiye

Mürüvvet Abbak
Türkiye

Gülen Melike Demirbolat
Türkiye

Mehran Aksel
Türkiye

Salih Paşa
Türkiye

Gizem Yalcin
Türkiye

Özge Çevik
Türkiye

Publication Date

June 28, 2025

Submission Date

June 7, 2021

Acceptance Date

August 26, 2021

Published in Issue

Year 2021 Volume: 25 Number: 5

IZ

https://izlik.org/JA43JR36GD

Cite

RIS / Bibtex

APA

Erdoğan, Ö., Abbak, M., Demirbolat, G. M., Aksel, M., Paşa, S., Yalcin, G., & Çevik, Ö. (2025). Treatment of glioblastoma by photodynamic therapy with the aid of synthesized silver nanoparticles by green chemistry from Citrus aurantium. Journal of Research in Pharmacy, 25(5), 641-652. https://izlik.org/JA43JR36GD

AMA

1.Erdoğan Ö, Abbak M, Demirbolat GM, et al. Treatment of glioblastoma by photodynamic therapy with the aid of synthesized silver nanoparticles by green chemistry from Citrus aurantium. J. Res. Pharm. 2025;25(5):641-652. https://izlik.org/JA43JR36GD

Chicago

Erdoğan, Ömer, Mürüvvet Abbak, Gülen Melike Demirbolat, et al. 2025. “Treatment of Glioblastoma by Photodynamic Therapy With the Aid of Synthesized Silver Nanoparticles by Green Chemistry from Citrus Aurantium”. Journal of Research in Pharmacy 25 (5): 641-52. https://izlik.org/JA43JR36GD.

EndNote

Erdoğan Ö, Abbak M, Demirbolat GM, Aksel M, Paşa S, Yalcin G, Çevik Ö (July 1, 2025) Treatment of glioblastoma by photodynamic therapy with the aid of synthesized silver nanoparticles by green chemistry from Citrus aurantium. Journal of Research in Pharmacy 25 5 641–652.

IEEE

[1]Ö. Erdoğan et al., “Treatment of glioblastoma by photodynamic therapy with the aid of synthesized silver nanoparticles by green chemistry from Citrus aurantium”, J. Res. Pharm., vol. 25, no. 5, pp. 641–652, July 2025, [Online]. Available: https://izlik.org/JA43JR36GD

ISNAD

Erdoğan, Ömer - Abbak, Mürüvvet - Demirbolat, Gülen Melike - Aksel, Mehran - Paşa, Salih - Yalcin, Gizem - Çevik, Özge. “Treatment of Glioblastoma by Photodynamic Therapy With the Aid of Synthesized Silver Nanoparticles by Green Chemistry from Citrus Aurantium”. Journal of Research in Pharmacy 25/5 (July 1, 2025): 641-652. https://izlik.org/JA43JR36GD.

JAMA

1.Erdoğan Ö, Abbak M, Demirbolat GM, Aksel M, Paşa S, Yalcin G, Çevik Ö. Treatment of glioblastoma by photodynamic therapy with the aid of synthesized silver nanoparticles by green chemistry from Citrus aurantium. J. Res. Pharm. 2025;25:641–652.

MLA

Erdoğan, Ömer, et al. “Treatment of Glioblastoma by Photodynamic Therapy With the Aid of Synthesized Silver Nanoparticles by Green Chemistry from Citrus Aurantium”. Journal of Research in Pharmacy, vol. 25, no. 5, July 2025, pp. 641-52, https://izlik.org/JA43JR36GD.

Vancouver

1.Ömer Erdoğan, Mürüvvet Abbak, Gülen Melike Demirbolat, Mehran Aksel, Salih Paşa, Gizem Yalcin, Özge Çevik. Treatment of glioblastoma by photodynamic therapy with the aid of synthesized silver nanoparticles by green chemistry from Citrus aurantium. J. Res. Pharm. [Internet]. 2025 Jul. 1;25(5):641-52. Available from: https://izlik.org/JA43JR36GD