Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection

Hüseyin Servi; Özgül Kısa; Ayhan Ibrahim Aysal; Gonca Erköse Genç; Dilek Şatana

Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection

Abstract

Healthcare-associated infection (HCAI) is a global health challenge and methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamases (ESBL) producing gram-negative bacilli, which were isolated from HCAIs, are the most common antimicrobial-resistant pathogens. The present research aimed to determine the chemical composition and antimicrobial activity of ethyl acetate and methanol extracts of Nigella sativa seeds. Antimicrobial activity of plant extracts was observed against 20 clinical yeast (which isolated from swab samples of tongue dorsal surfaces in patients with stomatitis), 18 clinical bacteria isolates (which found from HCAIs), and also eight bacteria, and three yeast standard strains by using a broth microdilution assay. The methanol extract had the highest inhibitory activity against S. aureus standard strain at 0.4 mg/mL. The ethyl acetate extract showed the highest antibacterial activity against Bacillus cereus standard strain at 0.4 mg/mL. The methanol and ethyl acetate extracts did not show inhibitory activity against Gram-negative bacteria and yeast isolates. The LC-Q-TOF-MS profiles of the extracts were found to contain saponin, alkaloid, and fatty acid. Mass spectra revealed the presence of 14 fatty acids, four saponins, and three alkaloids for methanol extract and 13 fatty acids, three saponins, and two alkaloids for ethyl acetate extract.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy, Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Hüseyin Servi ^*
0000-0002-4683-855X
Türkiye

Özgül Kısa
0000-0002-7160-4595
Türkiye

Ayhan Ibrahim Aysal This is me
0000-0001-8048-8621
Türkiye

Gonca Erköse Genç This is me
0000-0002-8983-9675
Türkiye

Dilek Şatana
0000-0002-8827-1504
Türkiye

Publication Date

June 28, 2025

Submission Date

September 10, 2021

Acceptance Date

December 30, 2021

Published in Issue

Year 2022 Volume: 26 Number: 2

IZ

https://izlik.org/JA28MC98XD

Cite

RIS / Bibtex

APA

Servi, H., Kısa, Ö., Aysal, A. I., Erköse Genç, G., & Şatana, D. (2025). Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. Journal of Research in Pharmacy, 26(2), 287-297. https://izlik.org/JA28MC98XD

AMA

1.Servi H, Kısa Ö, Aysal AI, Erköse Genç G, Şatana D. Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. J. Res. Pharm. 2025;26(2):287-297. https://izlik.org/JA28MC98XD

Chicago

Servi, Hüseyin, Özgül Kısa, Ayhan Ibrahim Aysal, Gonca Erköse Genç, and Dilek Şatana. 2025. “Chemical Profile by LC-Q-TOF-MS of Nigella Sativa Seed Extracts and in Vitro Antimicrobial Activity on Bacteria Which Are Determined Resistance Gene and Isolated from Nosocomial Infection”. Journal of Research in Pharmacy 26 (2): 287-97. https://izlik.org/JA28MC98XD.

EndNote

Servi H, Kısa Ö, Aysal AI, Erköse Genç G, Şatana D (June 1, 2025) Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. Journal of Research in Pharmacy 26 2 287–297.

IEEE

[1]H. Servi, Ö. Kısa, A. I. Aysal, G. Erköse Genç, and D. Şatana, “Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection”, J. Res. Pharm., vol. 26, no. 2, pp. 287–297, June 2025, [Online]. Available: https://izlik.org/JA28MC98XD

ISNAD

Servi, Hüseyin - Kısa, Özgül - Aysal, Ayhan Ibrahim - Erköse Genç, Gonca - Şatana, Dilek. “Chemical Profile by LC-Q-TOF-MS of Nigella Sativa Seed Extracts and in Vitro Antimicrobial Activity on Bacteria Which Are Determined Resistance Gene and Isolated from Nosocomial Infection”. Journal of Research in Pharmacy 26/2 (June 1, 2025): 287-297. https://izlik.org/JA28MC98XD.

JAMA

1.Servi H, Kısa Ö, Aysal AI, Erköse Genç G, Şatana D. Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. J. Res. Pharm. 2025;26:287–297.

MLA

Servi, Hüseyin, et al. “Chemical Profile by LC-Q-TOF-MS of Nigella Sativa Seed Extracts and in Vitro Antimicrobial Activity on Bacteria Which Are Determined Resistance Gene and Isolated from Nosocomial Infection”. Journal of Research in Pharmacy, vol. 26, no. 2, June 2025, pp. 287-9, https://izlik.org/JA28MC98XD.

Vancouver

1.Hüseyin Servi, Özgül Kısa, Ayhan Ibrahim Aysal, Gonca Erköse Genç, Dilek Şatana. Chemical profile by LC-Q-TOF-MS of Nigella sativa seed extracts and in vitro antimicrobial activity on bacteria which are determined resistance gene and isolated from nosocomial infection. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(2):287-9. Available from: https://izlik.org/JA28MC98XD