Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model

Didik Priyandoko; Wahyu Widowati; Agung Novianto; Hanna Sari Widya Kusuma; Viranda Andria Yuninda; Rizal Azis

Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model

Abstract

COVID-19 infection is always accompanied by a cytokine storm that causes acute respiratory distress syndrome (ARDS). The reduction of pro-inflammatory cytokines is expected to be used for treating COVID-19 patients. Guava (Psidium guajava L.) was believed to have properties as an antioxidant and anti-inflammatory. This study aims to determine the effect of guava extract (PGE) on the ARDS rats model so that it can be used as a PGE treatment for the effects caused by ARDS. This study was conducted on 25 Sprague Dawley rats model in 5 treatment groups. Lipopolysaccharides (LPS) induction was carried out to create ARDS rats model. Determination of pro-inflammatory cytokines in lung Interleukin-18 (IL-18), IL-12 and serum IL-1β, Tumour Necrosis Factor alpha (TNF-α) was performed by ELISA, NOD-, LRR- and Pyrin domain-containing protein 3 (NRLP3) expressions were determined by the quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) method and IL-6, Nuclear factor kappa B (NF-κB) were analysed by immunohisto-chemistry assay. The results showed that PGE contained phenolic compound, daidzin and genistin. The IL-18, IL-12, Il-1β, TNF-α, IL-6, NF-kB and NRLP3 in ARDS model rats were decreased by PGE treatment. In conclusion, PGE can be used as a therapy for treating ARDS by decreasing pro-inflammatory marker.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Didik Priyandoko
0000-0002-4945-237X
Indonesia

Wahyu Widowati ^*
0000-0002-5401-7794
Indonesia

Agung Novianto This is me
0000-0002-9658-835X
Indonesia

Hanna Sari Widya Kusuma
0000-0002-7422-0036
Indonesia

Viranda Andria Yuninda This is me
0009-0008-9025-2151
Indonesia

Rizal Azis
0000-0002-9605-0322
Indonesia

Publication Date

June 28, 2025

Submission Date

December 6, 2022

Acceptance Date

December 27, 2022

Published in Issue

Year 2024 Volume: 28 Number: 1

IZ

https://izlik.org/JA84TT63FB

Cite

RIS / Bibtex

APA

Priyandoko, D., Widowati, W., Novianto, A., Kusuma, H. S. W., Yuninda, V. A., & Azis, R. (2025). Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model. Journal of Research in Pharmacy, 28(1), 269-277. https://izlik.org/JA84TT63FB

AMA

1.Priyandoko D, Widowati W, Novianto A, Kusuma HSW, Yuninda VA, Azis R. Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model. J. Res. Pharm. 2025;28(1):269-277. https://izlik.org/JA84TT63FB

Chicago

Priyandoko, Didik, Wahyu Widowati, Agung Novianto, Hanna Sari Widya Kusuma, Viranda Andria Yuninda, and Rizal Azis. 2025. “Guava (Psidium Guajava L.) Extract Inhibits Cytokine Storm in Acute Respiratory Distress Syndrome Rats Model”. Journal of Research in Pharmacy 28 (1): 269-77. https://izlik.org/JA84TT63FB.

EndNote

Priyandoko D, Widowati W, Novianto A, Kusuma HSW, Yuninda VA, Azis R (June 1, 2025) Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model. Journal of Research in Pharmacy 28 1 269–277.

IEEE

[1]D. Priyandoko, W. Widowati, A. Novianto, H. S. W. Kusuma, V. A. Yuninda, and R. Azis, “Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model”, J. Res. Pharm., vol. 28, no. 1, pp. 269–277, June 2025, [Online]. Available: https://izlik.org/JA84TT63FB

ISNAD

Priyandoko, Didik - Widowati, Wahyu - Novianto, Agung - Kusuma, Hanna Sari Widya - Yuninda, Viranda Andria - Azis, Rizal. “Guava (Psidium Guajava L.) Extract Inhibits Cytokine Storm in Acute Respiratory Distress Syndrome Rats Model”. Journal of Research in Pharmacy 28/1 (June 1, 2025): 269-277. https://izlik.org/JA84TT63FB.

JAMA

1.Priyandoko D, Widowati W, Novianto A, Kusuma HSW, Yuninda VA, Azis R. Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model. J. Res. Pharm. 2025;28:269–277.

MLA

Priyandoko, Didik, et al. “Guava (Psidium Guajava L.) Extract Inhibits Cytokine Storm in Acute Respiratory Distress Syndrome Rats Model”. Journal of Research in Pharmacy, vol. 28, no. 1, June 2025, pp. 269-77, https://izlik.org/JA84TT63FB.

Vancouver

1.Didik Priyandoko, Wahyu Widowati, Agung Novianto, Hanna Sari Widya Kusuma, Viranda Andria Yuninda, Rizal Azis. Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model. J. Res. Pharm. [Internet]. 2025 Jun. 1;28(1):269-77. Available from: https://izlik.org/JA84TT63FB