Evaluation of herb-drug interaction of Withania somnifera with anti-rheumatic drugs through high-throughput screening

Satyajyoti Kanjilal; Ashok Kumar Gupta; Ranjana Patnaik; Amitabha Dey; Satyabrata Mohapatra

Evaluation of herb-drug interaction of Withania somnifera with anti-rheumatic drugs through high-throughput screening

Abstract

Rheumatoid Arthritis is an autoimmune inflammatory joint disease. Disease-modifying anti-rheumatic drugs and Non-steroidal anti-inflammatory drugs are being used for its management and found to have better improvement when combined with a traditional medicine. Withania somnifera (Ashwagandha) is one among the medicinally important herb which is known to be beneficial in these conditions. The root of the plant is safely being used however, very less is known about the aerial part usage; and their interactions with anti-rheumatic drugs. Cytochrome P450 enzymes inhibition assay for subtypes CYP3A4 and CYP2D6 isozyme was used in concoction of hydroalcoholic and methanolic extracts of root and aerial part of the plant with the anti-arthritic drugs. All the four Withania somnifera extracts showed significantly higher (P<0.001) IC50 value against CYP3A4 and CYP2D6 (IC50 >100µg/mL) compared to positive control ketoconazole and Quinidine respectively. Combination of formulation and anti-rheumatic drugs showed significantly (P<0.001) less inhibition on CYP3A4 and CYP2D6 as compared to positive control. To conclude, methanolic and hydroalcoholic extracts of both aerial and root part of Withania somnifera mixture with anti-rheumatic drug regimen had negligible CYP3A4 and CYP2D6 inhibition.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Sciences

Journal Section

Research Article

Authors

Satyajyoti Kanjilal ^* This is me
0000-0002-2328-1234
India

Ashok Kumar Gupta This is me
0000-0002-4369-4060
India

Ranjana Patnaik
India

Amitabha Dey This is me
India

Satyabrata Mohapatra This is me
India

Publication Date

June 28, 2025

Submission Date

August 7, 2021

Acceptance Date

February 19, 2022

Published in Issue

Year 2022 Volume: 26 Number: 2

IZ

https://izlik.org/JA79YK98SZ

Cite

RIS / Bibtex

APA

Kanjilal, S., Gupta, A. K., Patnaik, R., Dey, A., & Mohapatra, S. (2025). Evaluation of herb-drug interaction of Withania somnifera with anti-rheumatic drugs through high-throughput screening. Journal of Research in Pharmacy, 26(2), 334-344. https://izlik.org/JA79YK98SZ

AMA

1.Kanjilal S, Gupta AK, Patnaik R, Dey A, Mohapatra S. Evaluation of herb-drug interaction of Withania somnifera with anti-rheumatic drugs through high-throughput screening. J. Res. Pharm. 2025;26(2):334-344. https://izlik.org/JA79YK98SZ

Chicago

Kanjilal, Satyajyoti, Ashok Kumar Gupta, Ranjana Patnaik, Amitabha Dey, and Satyabrata Mohapatra. 2025. “Evaluation of Herb-Drug Interaction of Withania Somnifera With Anti-Rheumatic Drugs through High-Throughput Screening”. Journal of Research in Pharmacy 26 (2): 334-44. https://izlik.org/JA79YK98SZ.

EndNote

Kanjilal S, Gupta AK, Patnaik R, Dey A, Mohapatra S (June 1, 2025) Evaluation of herb-drug interaction of Withania somnifera with anti-rheumatic drugs through high-throughput screening. Journal of Research in Pharmacy 26 2 334–344.

IEEE

[1]S. Kanjilal, A. K. Gupta, R. Patnaik, A. Dey, and S. Mohapatra, “Evaluation of herb-drug interaction of Withania somnifera with anti-rheumatic drugs through high-throughput screening”, J. Res. Pharm., vol. 26, no. 2, pp. 334–344, June 2025, [Online]. Available: https://izlik.org/JA79YK98SZ

ISNAD

Kanjilal, Satyajyoti - Gupta, Ashok Kumar - Patnaik, Ranjana - Dey, Amitabha - Mohapatra, Satyabrata. “Evaluation of Herb-Drug Interaction of Withania Somnifera With Anti-Rheumatic Drugs through High-Throughput Screening”. Journal of Research in Pharmacy 26/2 (June 1, 2025): 334-344. https://izlik.org/JA79YK98SZ.

JAMA

1.Kanjilal S, Gupta AK, Patnaik R, Dey A, Mohapatra S. Evaluation of herb-drug interaction of Withania somnifera with anti-rheumatic drugs through high-throughput screening. J. Res. Pharm. 2025;26:334–344.

MLA

Kanjilal, Satyajyoti, et al. “Evaluation of Herb-Drug Interaction of Withania Somnifera With Anti-Rheumatic Drugs through High-Throughput Screening”. Journal of Research in Pharmacy, vol. 26, no. 2, June 2025, pp. 334-4, https://izlik.org/JA79YK98SZ.

Vancouver

1.Satyajyoti Kanjilal, Ashok Kumar Gupta, Ranjana Patnaik, Amitabha Dey, Satyabrata Mohapatra. Evaluation of herb-drug interaction of Withania somnifera with anti-rheumatic drugs through high-throughput screening. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(2):334-4. Available from: https://izlik.org/JA79YK98SZ