Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes

Ayse Tarbin Jannuzzı

Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes

Abstract

Eugenol is a phenolic compound isolated from clove essential oil. It is used in dentistry, fragrance, cosmetic, and food industries. According to the fragrance ingredient safety assessment report, eugenol does not cause phototoxic reactions and genotoxicity. However, its effect on UV-induced cytotoxicity and genotoxicity has not been well examined. Here in this study, cytotoxic and genotoxic effects of eugenol are investigated on UVA-induced damage using human keratinocyte cells (HaCaT). HaCaT cells were treated with increasing concentrations of eugenol (10-500 µM) for 1 hour and irradiated with 5-10-15 J/cm2 UVA. 24 hours later the neutral red uptake (NRU) assay was used to evaluate cytotoxicity. For genotoxicity assay cells were exposed to 1-10 µM eugenol for one hour and non-cytotoxic UVA irradiation doses (1, 2.5 J/cm2) were used. The alkaline comet assay was carried out immediately after the UVA irradiation to measure the genotoxic potential of eugenol. The cytotoxicity assay results indicate that eugenol caused a cytotoxic effect in a dose-dependent manner in HaCaT cells and increasing doses of UVA-irradiation enhanced the cytotoxic effect of eugenol. The alkaline comet assay results showed that eugenol causes DNA single-strand breaks and increasing doses of UVA-irradiation aggravates the genotoxic potential of eugenol. These data demonstrate that eugenol has cytotoxic and genotoxic potential and eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Toxicology

Journal Section

Research Article

Authors

Ayse Tarbin Jannuzzı ^*
0000-0003-0578-6893
Türkiye

Publication Date

June 28, 2025

Submission Date

September 8, 2021

Acceptance Date

December 30, 2021

Published in Issue

Year 2022 Volume: 26 Number: 2

IZ

https://izlik.org/JA36ZE98TT

Cite

RIS / Bibtex

APA

Jannuzzı, A. T. (2025). Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes. Journal of Research in Pharmacy, 26(2), 354-361. https://izlik.org/JA36ZE98TT

AMA

1.Jannuzzı AT. Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes. J. Res. Pharm. 2025;26(2):354-361. https://izlik.org/JA36ZE98TT

Chicago

Jannuzzı, Ayse Tarbin. 2025. “Eugenol Aggravates UVA-Induced Cytotoxic and Genotoxic Response in HaCaT Human Keratinocytes”. Journal of Research in Pharmacy 26 (2): 354-61. https://izlik.org/JA36ZE98TT.

EndNote

Jannuzzı AT (June 1, 2025) Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes. Journal of Research in Pharmacy 26 2 354–361.

IEEE

[1]A. T. Jannuzzı, “Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes”, J. Res. Pharm., vol. 26, no. 2, pp. 354–361, June 2025, [Online]. Available: https://izlik.org/JA36ZE98TT

ISNAD

Jannuzzı, Ayse Tarbin. “Eugenol Aggravates UVA-Induced Cytotoxic and Genotoxic Response in HaCaT Human Keratinocytes”. Journal of Research in Pharmacy 26/2 (June 1, 2025): 354-361. https://izlik.org/JA36ZE98TT.

JAMA

1.Jannuzzı AT. Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes. J. Res. Pharm. 2025;26:354–361.

MLA

Jannuzzı, Ayse Tarbin. “Eugenol Aggravates UVA-Induced Cytotoxic and Genotoxic Response in HaCaT Human Keratinocytes”. Journal of Research in Pharmacy, vol. 26, no. 2, June 2025, pp. 354-61, https://izlik.org/JA36ZE98TT.

Vancouver

1.Ayse Tarbin Jannuzzı. Eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT human keratinocytes. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(2):354-61. Available from: https://izlik.org/JA36ZE98TT