Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity

Ayça Güngör-ak; Esra Küpeli Akkol; Buket Aksu; Ayşegül Karataş

Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity

Abstract

Berberine (BER) is a benzylisoquinoline alkaloid found in many plants. It has high water solubilitybut its bioavailability is low in oral administration due to the first-pass effect in the liver and intestine. Phytosomesare are prepared by complexing herbal active ingredients and phospholipids (PLs). The aim of this study is to prepare BERphospholipidcomplexes (BPCs) using the reverse phase evaporation method. In the preparation of the formulations, the effects of BER:PL ratio (w/w), reaction temperature (°C) and reaction time (h) on the specifications of the complexes such as particle size (PS), zeta potential (ZP) and encapsulation efficiency (EE%) were investigated. The distribution of PS of obtained complexes was ranging between 339 -1259 nm and their ZP were within -4,27- -5,15 mV. The drug EE% was relatively high. Quality by Design (QbD) methods have been used in the formulation design and selection of the optimum formulation. BPCs anti-inflammatory, analgesic and antipyretic activities are evaluated by using in vivo methods.Carrageenan-, Prostaglandin E2 (PGE2)- and serotonin-induced hind paw edema, acetic acid-induced increase in capillary permeability and subcutaneous air-pouch models for the anti-inflammatory activity, inhibition of pbenzoquinone-induced abdominal constriction and hot plate test for the analgesic activity, Freund’s complete adjuvantinduced pyrexia model for the antipyretic activity were used in mice and rats. Results showed that BPCs showed potent anti-inflammatory, analgesic and antipyretic activities at the dose of 209 mg/kg.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacogenomics

Journal Section

Research Article

Authors

Ayça Güngör-ak This is me
0000-0002-7002-5209
Türkiye

Esra Küpeli Akkol
0000-0002-5829-7869
Türkiye

Buket Aksu
Türkiye

Ayşegül Karataş ^*
0000-0001-5157-5187
Türkiye

Publication Date

June 28, 2025

Submission Date

June 1, 2021

Acceptance Date

January 27, 2022

Published in Issue

Year 2022 Volume: 26 Number: 2

IZ

https://izlik.org/JA23RU68CP

Cite

RIS / Bibtex

APA

Güngör-ak, A., Küpeli Akkol, E., Aksu, B., & Karataş, A. (2025). Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. Journal of Research in Pharmacy, 26(2), 370-382. https://izlik.org/JA23RU68CP

AMA

1.Güngör-ak A, Küpeli Akkol E, Aksu B, Karataş A. Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. J. Res. Pharm. 2025;26(2):370-382. https://izlik.org/JA23RU68CP

Chicago

Güngör-ak, Ayça, Esra Küpeli Akkol, Buket Aksu, and Ayşegül Karataş. 2025. “Preparation and Optimization of Berberine Phospholipid Complexes Using QbD Approach and in Vivo Evaluation for Anti-Inflammatory, Analgesic and Antipyretic Activity”. Journal of Research in Pharmacy 26 (2): 370-82. https://izlik.org/JA23RU68CP.

EndNote

Güngör-ak A, Küpeli Akkol E, Aksu B, Karataş A (June 1, 2025) Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. Journal of Research in Pharmacy 26 2 370–382.

IEEE

[1]A. Güngör-ak, E. Küpeli Akkol, B. Aksu, and A. Karataş, “Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity”, J. Res. Pharm., vol. 26, no. 2, pp. 370–382, June 2025, [Online]. Available: https://izlik.org/JA23RU68CP

ISNAD

Güngör-ak, Ayça - Küpeli Akkol, Esra - Aksu, Buket - Karataş, Ayşegül. “Preparation and Optimization of Berberine Phospholipid Complexes Using QbD Approach and in Vivo Evaluation for Anti-Inflammatory, Analgesic and Antipyretic Activity”. Journal of Research in Pharmacy 26/2 (June 1, 2025): 370-382. https://izlik.org/JA23RU68CP.

JAMA

1.Güngör-ak A, Küpeli Akkol E, Aksu B, Karataş A. Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. J. Res. Pharm. 2025;26:370–382.

MLA

Güngör-ak, Ayça, et al. “Preparation and Optimization of Berberine Phospholipid Complexes Using QbD Approach and in Vivo Evaluation for Anti-Inflammatory, Analgesic and Antipyretic Activity”. Journal of Research in Pharmacy, vol. 26, no. 2, June 2025, pp. 370-82, https://izlik.org/JA23RU68CP.

Vancouver

1.Ayça Güngör-ak, Esra Küpeli Akkol, Buket Aksu, Ayşegül Karataş. Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(2):370-82. Available from: https://izlik.org/JA23RU68CP