Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum

Esra Eroğlu Özkan; Berna Özbek Çelik; Afife Mat

Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum

Abstract

Different crude extracts of some endemic Hypericum species (H. thymbrifolium, H. spectabile, H. pseudolaeve, H. neurocalycinum, H. malatyanum) and H. perforatum were analyzed using a microdilution assay for antimicrobial activity against several microorganisms. It is observed that all extracts showed activity against tested Gram positive bacteria (Staphylococcus aureus, Meticillin resistant S. aureus and Streptococcus epidermidis). The most active extracts were from H. neurocalycinum and H. malatyanum which showed potent activity with lowest MIC (4.8 µg/mL) value against more tested Gram positive bacteria. Additionally, it was also found that some extracts of H. spectabile and H. pseudolaeve had antifungal activities against C. albicans. These endemic species were evaluated for antimicrobial activity for the first time in the literature.

Keywords

References

[1] Özkan EE, Özsoy N, Özhan G, Celik BÖ, Mat A. Chemical composition and biological activities of Hypericum pamphylicum. Ind Crop Prod. 2013;50:182-189. [CrossRef]
[2] Sezik E, Yeşilada E, Honda G, Takaishi Y, Takeda Y, Tanaka T. Traditional medicine in Turkey X. Folk medicine in central Anatolia. J Ethnopharmacol. 2001;75(2-3):95-115. [CrossRef]
[3] Kültür Ş. Medicinal plants used in Kırklareli province (Turkey). J Ethnopharmacol. 2007;111(2):341-364. [CrossRef]
[4] Yeşilada E, Gürbüz Il, Shibata H. Screening of Turkish anti-ulcerogenic folk remedies for anti-Helicobacter pylori activity. J Ethnopharmacol. 1999;66(3):289-293. [CrossRef]
[5] Dell'Aica I, Garbisa S, Caniato R. The renaissance of Hypericum perforatum: bio-medical research catches up with folk medicine. Curr Bioact Compd. 2007;3(2):109-119. [CrossRef]
[6] Voss A, Verweij PE. Antibacterial activity of hyperforin from St John's wort. Lancet. 1999;354(9180):777. [CrossRef]
[7] Schempp CM, Pelz K, Wittmer A, Schöpf E, Simon JC. Antibacterial activity of hyperforin from St John's wort, against multiresistant Staphylococcus aureus and gram-positive bacteria. Lancet. 1999;353(9170):2129. [CrossRef]
[8] Saddiqe Z, Naeem I, Maimoona A. A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol. 2010;131(3):511-521. [CrossRef]

[9] Avato P, Raffo F, Guglielmi G, Vitali C, Rosato A. Extracts from St John's wort and their antimicrobial activity. Phytother Res. 2004;18(3):230-232. [CrossRef]
[10] Vajs V, Vugdelija S, Trifunović S, Karadzić I, Juranić N, Macura S, Milosavljević S. Further degradation product of hyperforin from Hypericum perforatum (St. John's Wort). Fitoterapia. 2003;74(5):439-444. [CrossRef]
[11] Barış D, Kızıl M, Aytekin Ç, Kızıl G, Yavuz M, Çeken B, Ertekin AS. In vitro antimicrobial and antioxidant activity of ethanol extract of three Hypericum and three Achillea species from Turkey. Int J Food Prop. 2011;14(2):339-355. [CrossRef]
[12] Sokmen A, Jones BM, Erturk M. Antimicrobial activity of extracts from the cell cultures of some Turkish medicinal plants. Phytother Res. 1999;13(4):355-357. [CrossRef]
[13] Unal EL, Mavi A, Kara AA, Cakir A, Şengül M, Yildirim A. Antimicrobial and antioxidant activities of some plants used as remedies in Turkish traditional medicine. Pharm Biol. 2008;46(3):207-224. [CrossRef]
[14] Turker AU, Yildirim AB, Karakas FP, Turker H. In vitro antibacterial and antitumor efficiency of some traditional plants from Turkey. Indian J Tradit Know. 2018;17(1):50-58. [CrossRef]
[15] Meral GE, Karabay N. In vitro antibacterial activities of three Hypericum species from West Anatolia. Turk Electron J Biotechnol. 2002:6-10. [CrossRef]
[16] Maltas E, Uysal A, Yildiztugay E, Aladag MO, Yildiz S, Kucukoduk M. Investigation of antioxidant and antibacterial activities of some Hypericum species. Fresenius Environ Bull. 2013;22(3):862-869.
[17] Del Monte D, De Martino L, Marandino A, Fratianni F, Nazzaro F, De Feo V. Phenolic content, antimicrobial and antioxidant activities of Hypericum perfoliatum L. Ind Crop Prod. 2015;74:342-347. [CrossRef]
[18] Marrelli M, Statti G, Conforti F, Menichini F. New potential pharmaceutical applications of hypericum species. Mini Rev Med Chem. 2016;16(9):710-720. [CrossRef]
[19] Li XM, Luo XG, Si CL, Wang N, Zhou H, He JF, Zhang TC. Antibacterial active compounds from Hypericum ascyron L. induce bacterial cell death through apoptosis pathway. Eur J Med Chem. 2015;96:436-444. [CrossRef]
[20] Conforti F, Statti G, Tundis R, Bianchi A, Agrimonti C, Sacchetti G, Andreotti E, Menichini F, Poli F. Comparative chemical composition and variability of biological activity of methanolic extracts from Hypericum perforatum L. Nat Prod Res. 2005;19(3):295-303. [CrossRef]
[21] Reichling J, Weseler A, Saller R. A current review of the antimicrobial activity of Hypericum perforatum L. Pharmacopsychiatry. 2001;34(1):116-118. [CrossRef]
[22] Rocha L, Marston A, Potterat O, Kaplan M, Hostettmann K. More phloroglucinols from Hypericum brasiliense. Phytochemistry. 1996;42(1):185-188. [CrossRef]
[23] Rocha L, Marston A, Potterat O, Kaplan MAC, Stoeckli-Evans H, Hostettmann K. Antibacterial phloroglucinols and flavonoids from Hypericum brasiliense. Phytochemistry. 1995;40(5):1447-1452. [CrossRef]
[24] Pistelli L, Bertoli A, Zucconelli S, Morelli I, Panizzi L, Menichini F. Antimicrobial activity of crude extracts and pure compounds of Hypericum hircinum. Fitoterapia. 2000;71:138-140. [CrossRef]
[25] Mukherjee PK, Saritha G, Suresh B. Antibacterial spectrum of Hypericum hookerianum. Fitoterapia. 2001;72(5):558-560. [CrossRef]
[26] Rabanal R, Arias A, Prado B, Hernández-Pérez M, Sánchez-Mateo C. Antimicrobial studies on three species of Hypericum from the Canary Islands. J Ethnopharmacol. 2002;81(2):287-292. [CrossRef]
[27] Matsuhisa M, Shikishima Y, Takaishi Y, Honda G, Ito M, Takeda Y, Shibata H, Higuti T, Kodzhimatov OK, Ashurmetov O. Benzoylphloroglucinol Derivatives from Hypericum scabrum. J Nat Prod. 2002;65(3):290-294. [CrossRef]
[28] Schempp CM, Windeck T, Hezel S, Simon JC. Topical treatment of atopic dermatitis with St. John's wort cream–a randomized, placebo controlled, double blind half-side comparison. Phytomedicine. 2003;10:31-37. [CrossRef]
[29] Dulger B, Gonuz A. Antibacterial activity of the endemic Hypericum kazdaghensis. Fitoterapia. 2005;76(2):237-239. [CrossRef]
[30] Gould IM, Bal AM. New antibiotic agents in the pipeline and how they can help overcome microbial resistance. Virulence. 2013;4(2):185-191. [CrossRef]
[31] Gu B, Kelesidis T, Tsiodras S, Hindler J, Humphries RM. The emerging problem of linezolid-resistant Staphylococcus. J Antimicrob Chemother. 2012;68(1):4-11. [CrossRef]
[32] (CLSI) CaLSI. Approved Standard M7-A7. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. PA, USA: CLSI: Wayne; 2006.
[33] (CLSI) CaLSI. 7th Informational Supplement; M100-S20. Performance Standards for Antimicrobial Susceptibility Testing. PA, USA: CLSI: Wayne; 2010

Details

Primary Language

English

Subjects

Pharmacognosy, Pharmaceutical Microbiology

Journal Section

Research Article

Authors

Esra Eroğlu Özkan ^*
Türkiye

Berna Özbek Çelik This is me
Türkiye

Afife Mat
Türkiye

Publication Date

June 27, 2025

Submission Date

February 26, 2018

Acceptance Date

May 15, 2018

Published in Issue

Year 2019 Volume: 23 Number: 1

IZ

https://izlik.org/JA27KL74NU

Cite

RIS / Bibtex

APA

Eroğlu Özkan, E., Özbek Çelik, B., & Mat, A. (2025). Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum. Journal of Research in Pharmacy, 23(1), 114-119. https://izlik.org/JA27KL74NU

AMA

1.Eroğlu Özkan E, Özbek Çelik B, Mat A. Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum. J. Res. Pharm. 2025;23(1):114-119. https://izlik.org/JA27KL74NU

Chicago

Eroğlu Özkan, Esra, Berna Özbek Çelik, and Afife Mat. 2025. “Antimicrobial Activities of Five Endemic Hypericum Species from Anatolia Compared With Hypericum Perforatum”. Journal of Research in Pharmacy 23 (1): 114-19. https://izlik.org/JA27KL74NU.

EndNote

Eroğlu Özkan E, Özbek Çelik B, Mat A (June 1, 2025) Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum. Journal of Research in Pharmacy 23 1 114–119.

IEEE

[1]E. Eroğlu Özkan, B. Özbek Çelik, and A. Mat, “Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum”, J. Res. Pharm., vol. 23, no. 1, pp. 114–119, June 2025, [Online]. Available: https://izlik.org/JA27KL74NU

ISNAD

Eroğlu Özkan, Esra - Özbek Çelik, Berna - Mat, Afife. “Antimicrobial Activities of Five Endemic Hypericum Species from Anatolia Compared With Hypericum Perforatum”. Journal of Research in Pharmacy 23/1 (June 1, 2025): 114-119. https://izlik.org/JA27KL74NU.

JAMA

1.Eroğlu Özkan E, Özbek Çelik B, Mat A. Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum. J. Res. Pharm. 2025;23:114–119.

MLA

Eroğlu Özkan, Esra, et al. “Antimicrobial Activities of Five Endemic Hypericum Species from Anatolia Compared With Hypericum Perforatum”. Journal of Research in Pharmacy, vol. 23, no. 1, June 2025, pp. 114-9, https://izlik.org/JA27KL74NU.

Vancouver

1.Esra Eroğlu Özkan, Berna Özbek Çelik, Afife Mat. Antimicrobial activities of five endemic Hypericum species from Anatolia compared with Hypericum perforatum. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(1):114-9. Available from: https://izlik.org/JA27KL74NU