Oxidative stress and urinary system damage in fructoseinduced rat model of metabolic syndrome: Effect of calorie restriction and exercise

Abstract

The aim of this study was to investigate the effects of calorie restriction (CR) and swimming exercise (SW) on oxidative injury in kidney and bladder tissues, in rats with metabolic syndrome (MS). 3-months old rats were divided into five; Control, MS, MS+CR, MS+SW, MS+CR+SW. The metabolic syndrome model was created using 10% fructose solution in drinking water for three months. Afterwards, SW and 40% CR were applied for six weeks. Blood glucose measurements were performed at the beginning, the third month and the end of experiment. After decapitation, blood, kidney and bladder samples were collected. Cytokine levels, antioxidant and oxidative stress parameters were examined. There was a remarkable change in blood glucose levels in MS+CR+SW group. Fructose-induced increased TNF-α and decreased ADP levels in plasma were reversed in CR, SW, and CR+SW groups. MDA levels were increased, while SOD and ADP levels were decreased in renal and bladder tissues in MS group. CR and SW significantly reversed all parameters in both tissues. Moreover, caspase activity increased in both tissues in MS group. CR decreased the caspase activity in kidney tissue, and in bladder tissues caspase activity significantly decreased with both CR and SW. Western blot analysis showed an increased caspase-3 protein expression in both tissues which was reversed by CR, SW, and CR+SW. The results of our study showed that MS disrupts the balance of pro/anti-inflammatory cytokines in plasma and causes oxidant damage in urinary tissues. Calorie restriction and exercise are protective against the damage caused by MS.

Keywords

• Metabolic syndrome
• exercise
• calorie restriction
• kidney
• bladder

References

1. [1] Spahis S, Borys JM, Levy E. Metabolic Syndrome as a Multifaceted Risk Factor for Oxidative Stress. Antioxid Redox Signal. 2017; 26(9): 445-61. [CrossRef]
2. [2] Kovesdy CP, Furth S, Zoccali C, World Kidney Day Steering C. Obesity and kidney disease: Hidden consequences of the epidemic. Indian J Nephrol. 2017; 27(2): 85-92. [CrossRef]
3. [3] Chang Y, Ryu S, Choi Y, Zhang Y, Cho J, Kwon MJ, Hyun YY, Lee KB, Kim H, Jung HS, Yun KE, Ahn J, Rampal S, Zhao D, Suh BS, Chung EC, Shin H, Pastor-Barriuso R, Guallar E. Metabolically Healthy Obesity and Development of Chronic Kidney Disease: A Cohort Study. Ann Intern Med. 2016; 164(5): 305-312. [CrossRef]
4. [4] La Russa D, Giordano F, Marrone A, Parafati M, Janda E, Pellegrino D. Oxidative Imbalance and Kidney Damage in Cafeteria Diet-Induced Rat Model of Metabolic Syndrome: Effect of Bergamot Polyphenolic Fraction. Antioxidants (Basel). 2019; 8(3). [CrossRef]
5. [5] Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52: 302-310. [CrossRef]
6. [6] Mylroie AA, Collins H, Umbles C, Kyle J. Erythrocyte superoxide dismutase activity and other parameters of copper status in rats ingesting lead acetate. Toxicol Appl Pharmacol. 1986; 82(3): 512-520. [CrossRef]
7. [7] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72: 248-254. [CrossRef]
8. [8] Guize L, Pannier B, Thomas F, Bean K, Jego B, Benetos A. Recent advances in metabolic syndrome and cardiovascular disease. Arch Cardiovasc Dis. 2008; 101(9): 577-583. [CrossRef]

9. [9] Huang PL. eNOS, metabolic syndrome and cardiovascular disease. Trends Endocrinol Metab. 2009; 20(6): 295-302. [CrossRef]
10. [10] Gorbachinsky I, Akpinar H, Assimos DG. Metabolic syndrome and urologic diseases. Rev Urol. 2010; 12(4): e157-80.
11. [11] He Q, Wang Z, Liu G, Daneshgari F, MacLennan GT, Gupta S. Metabolic syndrome, inflammation and lower urinary tract symptoms: possible translational links. Prostate Cancer Prostatic Dis. 2016; 19(1): 7-13. [CrossRef]
12. [12] McCracken E, Monaghan M, Sreenivasan S. Pathophysiology of the metabolic syndrome. Clin Dermatol. 2018; 36(1): 14-20. [CrossRef]
13. [13] Pouliot MC, Despres JP, Lemieux S, Moorjani S, Bouchard C, Tremblay A, et al. Waist circumference and abdominal sagittal diameter: best simple anthropometric indexes of abdominal visceral adipose tissue accumulation and related cardiovascular risk in men and women. Am J Cardiol. 1994; 73(7): 460-468. [CrossRef]
14. [14] Okamoto Y. Adiponectin provides cardiovascular protection in metabolic syndrome. Cardiol Res Pract. 2011; 2011: 313179. [CrossRef]
15. [15] Rodriguez A, Ezquerro S, Mendez-Gimenez L, Becerril S, Fruhbeck G. Revisiting the adipocyte: a model for integration of cytokine signaling in the regulation of energy metabolism. Am J Physiol Endocrinol Metab. 2015; 309(8): E691-714. [CrossRef]
16. [16] Clarkson PM. Antioxidants and physical performance. Crit Rev Food Sci Nutr. 1995; 35(1-2): 131-41. [CrossRef]
17. [17] Botezelli JD, Cambri LT, Ghezzi AC, Dalia RA, PP MS, Ribeiro C, et al. Different exercise protocols improve metabolic syndrome markers, tissue triglycerides content and antioxidant status in rats. Diabetol Metab Syndr. 2011; 3: 35. [CrossRef]
18. [18] Kannan K, Jain SK. Oxidative stress and apoptosis. Pathophysiology. 2000; 7(3): 153-63. [CrossRef]