Water fraction of Sonchus arvensis (Linn.) leaves protects heart upon isoproterenol-induced myocardial infarction in rats and promotes survival of cardiomyocytes in vitro

Neng Fisheri Kurniati; Elin Yulinah Sukandar; Rahmayati Rusnedy; Dhyan Kusuma Ayuningtyas; Nova Suliska; Yasushi Fujio

Water fraction of Sonchus arvensis (Linn.) leaves protects heart upon isoproterenol-induced myocardial infarction in rats and promotes survival of cardiomyocytes in vitro

Abstract

The study aimed to evaluate the cardioprotective role of Sonchus arvensis (Linn.) leaves in isoproterenol (ISO)-induced myocardial infarction (MI) in Wistar rats and in the survival of cardiomyocytes in vitro upon starvation challenge. Fractions of an ethanolic extract of S. arvensis leaves (100 and 200 mg/kg bw) were administered orally to Wistar rats (250–300 g) for 14 days. Isoproterenol 85 mg/kg bw was given at day 13 and 14. The rats were then sacrificed on the next day. Serum activities of aspartate transferase (AST), alanine transferase (ALT), creatine kinase (CK), creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) were measured using standard commercial kits. The protective effect of S. arvensis on damaged cardiomyocytes was investigated by examining the cell survival. Furthermore, the Tumor Necrosis Factor-α (TNF-α) serum level of the MI rats was also determined. The results show that pretreatment with n-hexane and ethyl acetate fractions of S. arvensis for a period of 14 days could not attenuate the increase of AST, ALT, CK, CK-MB, and LDH levels upon isoproterenol administration. Moreover, viability of the fractions pretreatedcardiomyocytes showed a cardiotoxic effect. However, pretreatment with the water fraction significantly attenuated the increase of AST, ALT, CK, CK-MB, and LDH levels in isoproterenol-induced MI rats and increased the survival of cardiomyocytes upon starvation challenge. The increase of the TNF-α level upon ISO administration in rats was not observed in the water fraction pre-treatment group. In conclusion, the water fraction of Sonchus arvensis L. leaves at a dose of 100 mg/kg bw protects the myocardium and exerts cardioprotective effects, probably by inhibiting TNF-α production.

Keywords

References

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Details

Primary Language

English

Subjects

Clinical Pharmacology and Therapeutics

Journal Section

Research Article

Authors

Neng Fisheri Kurniati ^*
Indonesia

Elin Yulinah Sukandar This is me
Indonesia

Rahmayati Rusnedy This is me
Indonesia

Dhyan Kusuma Ayuningtyas This is me
Indonesia

Nova Suliska
Indonesia

Yasushi Fujio This is me
Japan

Publication Date

June 27, 2025

Submission Date

March 26, 2018

Acceptance Date

July 18, 2018

Published in Issue

Year 2019 Volume: 23 Number: 2

IZ

https://izlik.org/JA59MF29BH

Cite

RIS / Bibtex

APA

Kurniati, N. F., Sukandar, E. Y., Rusnedy, R., Ayuningtyas, D. K., Suliska, N., & Fujio, Y. (2025). Water fraction of Sonchus arvensis (Linn.) leaves protects heart upon isoproterenol-induced myocardial infarction in rats and promotes survival of cardiomyocytes in vitro. Journal of Research in Pharmacy, 23(2), 242-248. https://izlik.org/JA59MF29BH

AMA

1.Kurniati NF, Sukandar EY, Rusnedy R, Ayuningtyas DK, Suliska N, Fujio Y. Water fraction of Sonchus arvensis (Linn.) leaves protects heart upon isoproterenol-induced myocardial infarction in rats and promotes survival of cardiomyocytes in vitro. J. Res. Pharm. 2025;23(2):242-248. https://izlik.org/JA59MF29BH

Chicago

Kurniati, Neng Fisheri, Elin Yulinah Sukandar, Rahmayati Rusnedy, Dhyan Kusuma Ayuningtyas, Nova Suliska, and Yasushi Fujio. 2025. “Water Fraction of Sonchus Arvensis (Linn.) Leaves Protects Heart Upon Isoproterenol-Induced Myocardial Infarction in Rats and Promotes Survival of Cardiomyocytes in Vitro”. Journal of Research in Pharmacy 23 (2): 242-48. https://izlik.org/JA59MF29BH.

EndNote

Kurniati NF, Sukandar EY, Rusnedy R, Ayuningtyas DK, Suliska N, Fujio Y (June 1, 2025) Water fraction of Sonchus arvensis (Linn.) leaves protects heart upon isoproterenol-induced myocardial infarction in rats and promotes survival of cardiomyocytes in vitro. Journal of Research in Pharmacy 23 2 242–248.

IEEE

[1]N. F. Kurniati, E. Y. Sukandar, R. Rusnedy, D. K. Ayuningtyas, N. Suliska, and Y. Fujio, “Water fraction of Sonchus arvensis (Linn.) leaves protects heart upon isoproterenol-induced myocardial infarction in rats and promotes survival of cardiomyocytes in vitro”, J. Res. Pharm., vol. 23, no. 2, pp. 242–248, June 2025, [Online]. Available: https://izlik.org/JA59MF29BH

ISNAD

Kurniati, Neng Fisheri - Sukandar, Elin Yulinah - Rusnedy, Rahmayati - Ayuningtyas, Dhyan Kusuma - Suliska, Nova - Fujio, Yasushi. “Water Fraction of Sonchus Arvensis (Linn.) Leaves Protects Heart Upon Isoproterenol-Induced Myocardial Infarction in Rats and Promotes Survival of Cardiomyocytes in Vitro”. Journal of Research in Pharmacy 23/2 (June 1, 2025): 242-248. https://izlik.org/JA59MF29BH.

JAMA

1.Kurniati NF, Sukandar EY, Rusnedy R, Ayuningtyas DK, Suliska N, Fujio Y. Water fraction of Sonchus arvensis (Linn.) leaves protects heart upon isoproterenol-induced myocardial infarction in rats and promotes survival of cardiomyocytes in vitro. J. Res. Pharm. 2025;23:242–248.

MLA

Kurniati, Neng Fisheri, et al. “Water Fraction of Sonchus Arvensis (Linn.) Leaves Protects Heart Upon Isoproterenol-Induced Myocardial Infarction in Rats and Promotes Survival of Cardiomyocytes in Vitro”. Journal of Research in Pharmacy, vol. 23, no. 2, June 2025, pp. 242-8, https://izlik.org/JA59MF29BH.

Vancouver

1.Neng Fisheri Kurniati, Elin Yulinah Sukandar, Rahmayati Rusnedy, Dhyan Kusuma Ayuningtyas, Nova Suliska, Yasushi Fujio. Water fraction of Sonchus arvensis (Linn.) leaves protects heart upon isoproterenol-induced myocardial infarction in rats and promotes survival of cardiomyocytes in vitro. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(2):242-8. Available from: https://izlik.org/JA59MF29BH