Acute hyperglycemia causes oxidative stress which is prevented by vitamin E pretreatment in healthy rabbits

Inci Kazkayası; Gokcen Tellı; Aylin Balci; Serdar Uma

Acute hyperglycemia causes oxidative stress which is prevented by vitamin E pretreatment in healthy rabbits

Abstract

Although there are many studies showing chronic hyperglycemia in diabetes increases oxidative stress, those examining the influence of acute glucose increase are limited. The aim of this study was to investigate the effect of acute hyperglycemia on oxidative stress markers in non-treated and vitamin E pretreated healthy rabbits. Acute hyperglycemia was induced by oral glucose administration (1g/kg). Before and at different time points up to 180 minutes following the administration, blood samples were collected to measure malondialdehyde and glutathione spectrophotometrically as oxidative stress and antioxidant capacity markers respectively. Same markers were also determined in kidney and liver homogenates. There was no difference in glucose tolerance between non-treated and vitamin E pre-treated groups (p=0.10). Plasma malondialdehyde levels were increased after glucose administration in both groups. There was a positive-correlation between blood glucose and plasma malondialdehyde in non-treated (r=+0.447; p=0.008) but not in vitamin E pre-treated group (r=0.076; p=0.361). Plasma glutathione levels were higher in vitamin E pre-treated group at all-time points compared to non-treated group (p<0.05). There was a negative-correlation between blood glucose and plasma glutathione levels in non-treated (r=- 0.357; p=0.033) but not in vitamin E pre-treated group (r=0.007; p=0.485). According to our results acute hyperglycemia provokes oxidative stress which is partially prevented by vitamin E pretreatment.

Keywords

References

[1] Choi SW, Benzie IF, Ma SW, Strain JJ, Hannigan BM. Acute hyperglycemia and oxidative stress: direct cause and effect? Free Radic Biol Med. 2008; 44(7): 1217-31. [CrossRef]
[2] Park CH, Noh JS, Kim JH, Tanaka T, Zhao Q, Matsumoto K, et al. Evaluation of Morroniside, Iridoid Glycoside from Corni Fructus, on Diabetes-Induced Alterations such as Oxidative Stress, Inflammation, and Apoptosis in the Liver of Type 2 Diabetic db/db Mice. Biol Pharm Bull. 2011; 34(10): 1559-65. [CrossRef]
[3] WHO Global report on diabetes 2016. https://www.who.int/publications/i/item/9789241565257, (accessed on 3 November 2021).
[4] Firuzi O, Miri R, Tavakkoli M, Saso L. Antioxidant therapy: current status and future prospects. Curr Med Chem. 2011; 18(25): 3871-88. [CrossRef]
[5] Ghosh A, Sil PC. A protein from Cajanus indicus Spreng protects liver and kidney against mercuric chloride-induced oxidative stress. Biol Pharm Bull. 2008; 31(9): 1651-8. [CrossRef]
[6] Wu N, Shen H, Liu H, Wang Y, Bai Y, Han P. Acute blood glucose fluctuation enhances rat aorta endothelial cell apoptosis, oxidative stress and pro-inflammatory cytokine expression in vivo. Cardiovasc Diabetol. 2016; 15(1): 109. [CrossRef]
[7] Ling PR, Smith RJ, Bistrian BR. Acute effects of hyperglycemia and hyperinsulinemia on hepatic oxidative stress and the systemic inflammatory response in rats. Crit Care Med. 2007; 35(2): 555-60. [CrossRef]
[8] Su H, Ji L, Xing W, Zhang W, Zhou H, Qian X, et al. Acute hyperglycaemia enhances oxidative stress and aggravates myocardial ischaemia/reperfusion injury: role of thioredoxin-interacting protein. J Cell Mol Med. 2013; 17(1): 181-91. [CrossRef]

[9] Yang Z, Laubach VE, French BA, Kron IL. Acute hyperglycemia enhances oxidative stress and exacerbates myocardial infarction by activating nicotinamide adenine dinucleotide phosphate oxidase during reperfusion. J Thorac Cardiovasc Surg. 2009; 137(3): 723-9. [CrossRef]
[10] Ceriello A, Bortolotti N, Crescentini A, Motz E, Lizzio S, Russo A, et al. Antioxidant defences are reduced during the oral glucose tolerance test in normal and non-insulin-dependent diabetic subjects. Eur J Clin Invest. 1998; 28(4): 329-33. [CrossRef]
[11] Shen XP, Li J, Zou S, Wu HJ, Zhang Y. The relationship between oxidative stress and the levels of serum circulating adhesion molecules in patients with hyperglycemia crises. J Diabetes Complications. 2012; 26(4): 291-5. [CrossRef]
[12] Ceriello A, Novials A, Ortega E, Canivell S, La Sala L, Pujadas G, et al. Glucagon-like peptide 1 reduces endothelial dysfunction, inflammation, and oxidative stress induced by both hyperglycemia and hypoglycemia in type 1 diabetes. Diabetes Care. 2013; 36(8): 2346-50. [CrossRef]
[13] Ashor AW, Chowdhury S, Oggioni C, Qadir O, Brandt K, Ishaq A, Mathers JC, Saretzki G, Siervo M. Inorganic Nitrate Supplementation in Young and Old Obese Adults Does Not Affect Acute Glucose and Insulin Responses but Lowers Oxidative Stress. J Nutr. 2016; 146(11): 2224-32. [CrossRef]
[14] Balbi ME, Tonin FS, Mendes AM, Borba HH, Wiens A, Fernandez-Llimos F, Pontarolo R. Antioxidant effects of vitamins in type 2 diabetes: a meta-analysis of randomized controlled trials. Diabetol Metab Syndr. 2018; 10: 18. [CrossRef]
[15] Picklo MJ, Thyfault JP. Vitamin E and vitamin C do not reduce insulin sensitivity but inhibit mitochondrial protein expression in exercising obese rats. Appl Physiol Nutr Metab. 2015; 40(4): 343-52. [CrossRef]
[16] Sanyal AJ, Kowdley KV, Neuschwander-Tetri BA, Brunt EM, McCullough AJ, Bass NM, Diehl AM, Unalp-Arida A, Chalasani N. Relationship between adipose tissue insulin resistance and liver histology in nonalcoholic steatohepatitis: a pioglitazone versus vitamin E versus placebo for the treatment of nondiabetic patients with nonalcoholic steatohepatitis trial follow-up study. Hepatology. 2012; 56(4): 1311-8. [CrossRef]
[17] Wong SK, Chin KY, Suhaimi FH, Ahmad F, Ima-Nirwana S. Vitamin E As a Potential Interventional Treatment for Metabolic Syndrome: Evidence from Animal and Human Studies. Front Pharmacol. 2017; 8: 444. [CrossRef]
[18] Xu R, Zhang S, Tao A, Chen G, Zhang M. Influence of vitamin E supplementation on glycaemic control: a meta-analysis of randomised controlled trials. PLoS One. 2014; 9(4): e95008. [CrossRef]
[19] Polyzos SA, Kountouras J, Zafeiriadou E, Patsiaoura K, Katsiki E, Deretzi G, et al. Effect of spironolactone and vitamin E on serum metabolic parameters and insulin resistance in patients with nonalcoholic fatty liver disease. J Renin Angiotensin Aldosterone Syst. 2011; 12(4): 498-503. [CrossRef]
[20] Ebtehal K Farrag NSM, Amany S Maghraby, Iman M Mourad, Doaa S Foda, Sayed M Raw. Does the Simultaneous Use of Selenium and Vitamin E Improve Type 2 Diabetes Status? Int J Pharm Sci Rev Res. 2014; 26(2): 129-35.
[21] Al Shamsi MS, Amin A, Adeghate E. Beneficial effect of vitamin E on the metabolic parameters of diabetic rats. Mol Cell Biochem. 2004; 261(1-2): 35-42. [CrossRef]
[22] van Haaften RI, Haenen GR, Evelo CT, Bast A. Effect of vitamin E on glutathione-dependent enzymes. Drug Metab Rev. 2003; 35(2-3): 215-53. [CrossRef]
[23] Yoshida M, Kimura H, Kyuki K, Ito M. Effect of combined vitamin E and insulin administration on renal damage in diabetic rats fed a high cholesterol diet. Biol Pharm Bull. 2005; 28(11): 2080-6. [CrossRef]
[24] Ferraz FF, Kos AG, Janino P, Homsi E. Effects of melatonin administration to rats with glycerol-induced acute renal failure. Ren Fail. 2002; 24(6): 735-46. [CrossRef]
[25] Karamalakova YD, Nikolova GD, Georgiev TK, Gadjeva VG, Tolekova AN. Hepatoprotective properties of Curcuma longa L. extract in bleomycin-induced chronic hepatotoxicity. Drug Discov Ther. 2019; 13(1): 9-16. [CrossRef]
[26] Yazar E, Konyalioglu S, Col R, Birdane YO, Bas AL, Elmas M. Effects of vitamin E and prednisolone on some oxidative stress markers in endotoxemic rabbits. Rev Med Vet-Toulouse. 2004; 155(11): 538-42.
[27] Sedlak J, Lindsay RH. Estimation of Total Protein-Bound and Nonprotein Sulfhydryl Groups in Tissue with Ellmans Reagent. Anal Biochem. 1968; 25(1-3): 192. [CrossRef]
[28] Baila B, Ohno Y, Nagamoto H, Kotosai K, Yabuuchi Y, Funaguchi N, Ito F, Endo J, Mori H, Takemura G, Fujiwara T, Fujiwara H, Minatoguchi S. Tetomilast Attenuates Elastase-Induced Pulmonary Emphysema through Inhibition of Oxidative Stress in Rabbits. Biol Pharm Bull. 2012; 35(4): 494-502. [CrossRef]

Details

Primary Language

English

Subjects

Pharmaceutical Toxicology, Basic Pharmacology

Journal Section

Research Article

Authors

Inci Kazkayası ^*
0000-0003-1159-9680
Türkiye

Gokcen Tellı
0000-0003-0028-6769
Türkiye

Aylin Balci This is me
Türkiye

Serdar Uma
0000-0003-4064-6319
Türkiye

Publication Date

June 28, 2025

Submission Date

November 4, 2021

Acceptance Date

February 2, 2022

Published in Issue

Year 2022 Volume: 26 Number: 3

IZ

https://izlik.org/JA48DF33AE

Cite

RIS / Bibtex

APA

Kazkayası, I., Tellı, G., Balci, A., & Uma, S. (2025). Acute hyperglycemia causes oxidative stress which is prevented by vitamin E pretreatment in healthy rabbits. Journal of Research in Pharmacy, 26(3), 534-542. https://izlik.org/JA48DF33AE

AMA

1.Kazkayası I, Tellı G, Balci A, Uma S. Acute hyperglycemia causes oxidative stress which is prevented by vitamin E pretreatment in healthy rabbits. J. Res. Pharm. 2025;26(3):534-542. https://izlik.org/JA48DF33AE

Chicago

Kazkayası, Inci, Gokcen Tellı, Aylin Balci, and Serdar Uma. 2025. “Acute Hyperglycemia Causes Oxidative Stress Which Is Prevented by Vitamin E Pretreatment in Healthy Rabbits”. Journal of Research in Pharmacy 26 (3): 534-42. https://izlik.org/JA48DF33AE.

EndNote

Kazkayası I, Tellı G, Balci A, Uma S (June 1, 2025) Acute hyperglycemia causes oxidative stress which is prevented by vitamin E pretreatment in healthy rabbits. Journal of Research in Pharmacy 26 3 534–542.

IEEE

[1]I. Kazkayası, G. Tellı, A. Balci, and S. Uma, “Acute hyperglycemia causes oxidative stress which is prevented by vitamin E pretreatment in healthy rabbits”, J. Res. Pharm., vol. 26, no. 3, pp. 534–542, June 2025, [Online]. Available: https://izlik.org/JA48DF33AE

ISNAD

Kazkayası, Inci - Tellı, Gokcen - Balci, Aylin - Uma, Serdar. “Acute Hyperglycemia Causes Oxidative Stress Which Is Prevented by Vitamin E Pretreatment in Healthy Rabbits”. Journal of Research in Pharmacy 26/3 (June 1, 2025): 534-542. https://izlik.org/JA48DF33AE.

JAMA

1.Kazkayası I, Tellı G, Balci A, Uma S. Acute hyperglycemia causes oxidative stress which is prevented by vitamin E pretreatment in healthy rabbits. J. Res. Pharm. 2025;26:534–542.

MLA

Kazkayası, Inci, et al. “Acute Hyperglycemia Causes Oxidative Stress Which Is Prevented by Vitamin E Pretreatment in Healthy Rabbits”. Journal of Research in Pharmacy, vol. 26, no. 3, June 2025, pp. 534-42, https://izlik.org/JA48DF33AE.

Vancouver

1.Inci Kazkayası, Gokcen Tellı, Aylin Balci, Serdar Uma. Acute hyperglycemia causes oxidative stress which is prevented by vitamin E pretreatment in healthy rabbits. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(3):534-42. Available from: https://izlik.org/JA48DF33AE