Antioxidant, anticancer activities, and HPLC-DAD analyses of the medicinal halophyte Limoniastrum guyonianum Dur. extracts

Sara Zerrouki; Samia Mezhoud; Ayse Sahin Yağlioğlu; Chawki Bensouici; Mehmet Nuri Atalar; İbrahim Demirtas; Souad Ameddah; Ratiba Mekkiou

Antioxidant, anticancer activities, and HPLC-DAD analyses of the medicinal halophyte Limoniastrum guyonianum Dur. extracts

Abstract

Although notable medicinal value and biological activities such as antioxidant, antibacterial, antimicrobial and antifungal of Limoniastrum guyonianum have been reported, identification and quantification of phytoconstituents of CHCl3, EtOAC, and n-BuOH extracts from the aerial parts of the halophyte medicinal plant from Algerian Sahara was not well investigated for biological and anticancer activity in spite of their great interest. Therefore, the aim of this study was to investigate the antioxidant and anticancer activities of L. guyonianum. The phytoconstituents of CHCl3, EtOAC, and n-BuOH extracts were screened using HPLC-DAD. Total phenolic and flavonoid contents were quantified, antioxidant activity, using DPPH radical scavenging activity, ABTS+° decolorization, cupric reducing power, and anticancer activity against human cervical adenocarcinoma (HeLa) cell line were investigated and quantified. The results showed the presence of chlorogenic, gallic, syringic, p-coumaric, trans ferulic, o-coumaric acids, and quercetin in significant varying quantities. Chlorogenic acid was present in the greatest quantity (344.027–422.711mg/g of extract) as well as quercetin in CHCl3 extract. It is important to note the absence of caffeic acid and o-coumaric acid from EtOAc and CHCl3 extracts, respectively. The content of phenolic and flavonoid were higher in EtOAc extract (934±4,38mgGAE and 218.33±6.36mgQE/gof extract), respectively. EtOAc extract possesses a strong antioxidant effect in vitro, while the CHCl3 extract proved to be the most active againstHeLa cell lines (IC50= 50.369±0.020μg/ml). The findings in this studydemostrate the potential use of these compounds in medicine and importance of this species, which have largely been used in folk medicine for several diseases’ treatment.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry

Journal Section

Research Article

Authors

Sara Zerrouki This is me
0000-0003-1266-5280
Algeria

Samia Mezhoud This is me
0000-0002-1698-5188
Algeria

Ayse Sahin Yağlioğlu This is me
0000-0002-5283-3501
Türkiye

Chawki Bensouici This is me
0000-0003-4612-4642
Algeria

Mehmet Nuri Atalar
0000-0003-2993-2605
Türkiye

İbrahim Demirtas
0000-0001-8946-647X
Türkiye

Souad Ameddah This is me
0000-0001-9115-6972
Algeria

Ratiba Mekkiou ^* This is me
Algeria

Publication Date

June 28, 2025

Submission Date

August 2, 2021

Acceptance Date

February 28, 2022

Published in Issue

Year 2022 Volume: 26 Number: 3

IZ

https://izlik.org/JA67FY24UZ

Cite

RIS / Bibtex

APA

Zerrouki, S., Mezhoud, S., Sahin Yağlioğlu, A., Bensouici, C., Atalar, M. N., Demirtas, İ., Ameddah, S., & Mekkiou, R. (2025). Antioxidant, anticancer activities, and HPLC-DAD analyses of the medicinal halophyte Limoniastrum guyonianum Dur. extracts. Journal of Research in Pharmacy, 26(3), 598-608. https://izlik.org/JA67FY24UZ

AMA

1.Zerrouki S, Mezhoud S, Sahin Yağlioğlu A, et al. Antioxidant, anticancer activities, and HPLC-DAD analyses of the medicinal halophyte Limoniastrum guyonianum Dur. extracts. J. Res. Pharm. 2025;26(3):598-608. https://izlik.org/JA67FY24UZ

Chicago

Zerrouki, Sara, Samia Mezhoud, Ayse Sahin Yağlioğlu, et al. 2025. “Antioxidant, Anticancer Activities, and HPLC-DAD Analyses of the Medicinal Halophyte Limoniastrum Guyonianum Dur. Extracts”. Journal of Research in Pharmacy 26 (3): 598-608. https://izlik.org/JA67FY24UZ.

EndNote

Zerrouki S, Mezhoud S, Sahin Yağlioğlu A, Bensouici C, Atalar MN, Demirtas İ, Ameddah S, Mekkiou R (June 1, 2025) Antioxidant, anticancer activities, and HPLC-DAD analyses of the medicinal halophyte Limoniastrum guyonianum Dur. extracts. Journal of Research in Pharmacy 26 3 598–608.

IEEE

[1]S. Zerrouki et al., “Antioxidant, anticancer activities, and HPLC-DAD analyses of the medicinal halophyte Limoniastrum guyonianum Dur. extracts”, J. Res. Pharm., vol. 26, no. 3, pp. 598–608, June 2025, [Online]. Available: https://izlik.org/JA67FY24UZ

ISNAD

Zerrouki, Sara - Mezhoud, Samia - Sahin Yağlioğlu, Ayse - Bensouici, Chawki - Atalar, Mehmet Nuri - Demirtas, İbrahim - Ameddah, Souad - Mekkiou, Ratiba. “Antioxidant, Anticancer Activities, and HPLC-DAD Analyses of the Medicinal Halophyte Limoniastrum Guyonianum Dur. Extracts”. Journal of Research in Pharmacy 26/3 (June 1, 2025): 598-608. https://izlik.org/JA67FY24UZ.

JAMA

1.Zerrouki S, Mezhoud S, Sahin Yağlioğlu A, Bensouici C, Atalar MN, Demirtas İ, Ameddah S, Mekkiou R. Antioxidant, anticancer activities, and HPLC-DAD analyses of the medicinal halophyte Limoniastrum guyonianum Dur. extracts. J. Res. Pharm. 2025;26:598–608.

MLA

Zerrouki, Sara, et al. “Antioxidant, Anticancer Activities, and HPLC-DAD Analyses of the Medicinal Halophyte Limoniastrum Guyonianum Dur. Extracts”. Journal of Research in Pharmacy, vol. 26, no. 3, June 2025, pp. 598-0, https://izlik.org/JA67FY24UZ.

Vancouver

1.Sara Zerrouki, Samia Mezhoud, Ayse Sahin Yağlioğlu, Chawki Bensouici, Mehmet Nuri Atalar, İbrahim Demirtas, Souad Ameddah, Ratiba Mekkiou. Antioxidant, anticancer activities, and HPLC-DAD analyses of the medicinal halophyte Limoniastrum guyonianum Dur. extracts. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(3):598-60. Available from: https://izlik.org/JA67FY24UZ