Phosholipid trafficking between serum-HDL-erythrocyte in various conditions associated with circadian disturbances

Fatma Ölmez Budak; Emine Koç; Saliha Büyüktuncel; Harika Gözükara Bağ; Serpil Şener; Bahri Evren; İbrahim Şahin; Kürşat Kaya; Tayfun Güldür

Phosholipid trafficking between serum-HDL-erythrocyte in various conditions associated with circadian disturbances

Abstract

Circadian disturbances are known to affect lipid metabolism. Most of the phospholipid metabolites have been shown to be under circaian control by metabolomics studies. Moreover, genes related to glycerolipid synthesis were reported to be circadianly regulated. Data from various studies revealed a relationship between glycerolipid metabolism and circadian misalignments. Some proteins responsible for transfer of phospholipids among plasma lipoproteins or membranes include phospholipid transfer proteins (PLTP), lecithin cholesterol acyltransferase (LCAT), secretory phospholipase A2 (sPLA2), endothelial lipase (EL), exhibits circadian oscillation. However no data are available as to whether circadian disturbances can influence phospholipid trafficking among HDL, erythrocytes and serum. To this end, , four conditions associated with circadian disturbances including type 2 diabetes, prediabetes with metformin usage, psoriasis and night-shift work were investigated for phospholipid trafficking. Indices of circadian misalignments, plasma melatonin and cortisol levels, were determined by ELISA and chemiluminescence methods respectively. Serum levels of PLTP, LCAT, EL and sPLA2 levels were analyzed by ELISA. Phospholipid compositions were investigated by two-dimensional HPTLC and/or HPLC. Results by HPLC indicated that PE/PC ratios in erythrocyte lysates of diabetes and metformin groups were found to be significantly lower compared to that of controls which might be associated with the lower levels of LCAT, EL and PLTP levels measured. Altered plasma melatonin levels indicated circadian misalignments in these conditions. However, in psoriasis and night-shift groups, circadian indexes did not match with the PE/PC ratios in erythrocytes as it was in diabetes and metformin groups. We therefore conclude that circadian as well as metabolic disturbances both might have a role in phospholipid trafficking.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry

Journal Section

Research Article

Authors

Fatma Ölmez Budak ^*
0000-0003-4196-9224
Türkiye

Emine Koç
0000-0003-3122-7104
Türkiye

Saliha Büyüktuncel
0000-0001-7269-6594
Türkiye

Harika Gözükara Bağ
0000-0003-1208-4072
Türkiye

Serpil Şener This is me
0000-0002-7012-2666
Türkiye

Bahri Evren
0000-0001-7490-2937
Türkiye

İbrahim Şahin
0000-0002-6231-0034
Türkiye

Kürşat Kaya
0000-0002-6353-7791
Türkiye

Tayfun Güldür
0000-0002-1623-2880
Türkiye

Publication Date

June 28, 2025

Submission Date

January 31, 2024

Acceptance Date

March 22, 2024

Published in Issue

Year 2024 Volume: 28 Number: 4

IZ

https://izlik.org/JA83MW42AW

Cite

RIS / Bibtex

APA

Ölmez Budak, F., Koç, E., Büyüktuncel, S., Gözükara Bağ, H., Şener, S., Evren, B., Şahin, İ., Kaya, K., & Güldür, T. (2025). Phosholipid trafficking between serum-HDL-erythrocyte in various conditions associated with circadian disturbances. Journal of Research in Pharmacy, 28(4), 1107-1123. https://izlik.org/JA83MW42AW

AMA

1.Ölmez Budak F, Koç E, Büyüktuncel S, et al. Phosholipid trafficking between serum-HDL-erythrocyte in various conditions associated with circadian disturbances. J. Res. Pharm. 2025;28(4):1107-1123. https://izlik.org/JA83MW42AW

Chicago

Ölmez Budak, Fatma, Emine Koç, Saliha Büyüktuncel, et al. 2025. “Phosholipid Trafficking Between Serum-HDL-Erythrocyte in Various Conditions Associated With Circadian Disturbances”. Journal of Research in Pharmacy 28 (4): 1107-23. https://izlik.org/JA83MW42AW.

EndNote

Ölmez Budak F, Koç E, Büyüktuncel S, Gözükara Bağ H, Şener S, Evren B, Şahin İ, Kaya K, Güldür T (July 1, 2025) Phosholipid trafficking between serum-HDL-erythrocyte in various conditions associated with circadian disturbances. Journal of Research in Pharmacy 28 4 1107–1123.

IEEE

[1]F. Ölmez Budak et al., “Phosholipid trafficking between serum-HDL-erythrocyte in various conditions associated with circadian disturbances”, J. Res. Pharm., vol. 28, no. 4, pp. 1107–1123, July 2025, [Online]. Available: https://izlik.org/JA83MW42AW

ISNAD

Ölmez Budak, Fatma - Koç, Emine - Büyüktuncel, Saliha - Gözükara Bağ, Harika - Şener, Serpil - Evren, Bahri - Şahin, İbrahim - Kaya, Kürşat - Güldür, Tayfun. “Phosholipid Trafficking Between Serum-HDL-Erythrocyte in Various Conditions Associated With Circadian Disturbances”. Journal of Research in Pharmacy 28/4 (July 1, 2025): 1107-1123. https://izlik.org/JA83MW42AW.

JAMA

1.Ölmez Budak F, Koç E, Büyüktuncel S, Gözükara Bağ H, Şener S, Evren B, Şahin İ, Kaya K, Güldür T. Phosholipid trafficking between serum-HDL-erythrocyte in various conditions associated with circadian disturbances. J. Res. Pharm. 2025;28:1107–1123.

MLA

Ölmez Budak, Fatma, et al. “Phosholipid Trafficking Between Serum-HDL-Erythrocyte in Various Conditions Associated With Circadian Disturbances”. Journal of Research in Pharmacy, vol. 28, no. 4, July 2025, pp. 1107-23, https://izlik.org/JA83MW42AW.

Vancouver

1.Fatma Ölmez Budak, Emine Koç, Saliha Büyüktuncel, Harika Gözükara Bağ, Serpil Şener, Bahri Evren, İbrahim Şahin, Kürşat Kaya, Tayfun Güldür. Phosholipid trafficking between serum-HDL-erythrocyte in various conditions associated with circadian disturbances. J. Res. Pharm. [Internet]. 2025 Jul. 1;28(4):1107-23. Available from: https://izlik.org/JA83MW42AW