Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye)

Selçuk Sevinç; Mehmet Gökhan Halıcı; Ahmet Cumaoğlu

Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye)

Abstract

In this study, we investigated the anti-microbial and anti-inflammatory activities of the cosmopolite macrolichens Usnea articulata (L.) Hoffm., Umbilicaria crustulosa (Ach.) Lamy and Bryoria fuscescens (Gyeln.) Brodo & D.Hawksw hydroalcoholic extracts to contribute the potential pharmacological uses of lichens. In vitro antimicrobial activities of ethanol extracts against Gram-negative bacteria Escherichia coli, Gram-positive bacteria Staphylococcus aureus, and the yeast Candida albicans were presented using the Broth microdilution method. The most effective lichen extract against gram-positive bacteria S. aureus was U. articulata ethanol extract with a MIC value of 0.125 mg/ml. U. articulata and B. fuscences extracts have similar anti-fungal activities despite having MIC values of 0.5 mg/ml. The anti- inflammatory effects of the extracts on Lipopolysaccharide/Interferon-gamma (LPS/IF-γ) induced macrophage-like cellular systems (BV-2 microglia and RAW 264,7 macrophages) were evaluated by measuring P38 mitogen-activated protein kinase phosphorylation (P38MAPK), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase 2 (NOS2) mRNA and protein expression. Especially, Usnea and Umbilicaria extracts also attenuated the LPS/IF-γ induced increase in P38MAPK phosphorylation, COX-2, and NOS2 expression in both macrophage-like cells without any cytotoxicity. According to the results of our study, we suggest that the anti-inflammatory mechanism of lichen extracts might result from the inhibition of P38MAPK phosphorylation through a reduction in COX-2 and NOS2 expressions.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Selçuk Sevinç This is me
0009-0006-9216-5751
Türkiye

Mehmet Gökhan Halıcı
0000-0003-4797-1157
Türkiye

Ahmet Cumaoğlu ^* This is me
0000-0002-3997-7746
Türkiye

Publication Date

June 28, 2025

Submission Date

September 13, 2023

Acceptance Date

November 29, 2023

Published in Issue

Year 2024 Volume: 28 Number: 4

IZ

https://izlik.org/JA55TF83MR

Cite

RIS / Bibtex

APA

Sevinç, S., Halıcı, M. G., & Cumaoğlu, A. (2025). Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye). Journal of Research in Pharmacy, 28(4), 1166-1173. https://izlik.org/JA55TF83MR

AMA

1.Sevinç S, Halıcı MG, Cumaoğlu A. Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye). J. Res. Pharm. 2025;28(4):1166-1173. https://izlik.org/JA55TF83MR

Chicago

Sevinç, Selçuk, Mehmet Gökhan Halıcı, and Ahmet Cumaoğlu. 2025. “Screening of Antimicrobial and Antiinflammatory Activities of Three Lichenized Fungal Extracts Collected from Northwest Anatolia (Türkiye)”. Journal of Research in Pharmacy 28 (4): 1166-73. https://izlik.org/JA55TF83MR.

EndNote

Sevinç S, Halıcı MG, Cumaoğlu A (July 1, 2025) Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye). Journal of Research in Pharmacy 28 4 1166–1173.

IEEE

[1]S. Sevinç, M. G. Halıcı, and A. Cumaoğlu, “Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye)”, J. Res. Pharm., vol. 28, no. 4, pp. 1166–1173, July 2025, [Online]. Available: https://izlik.org/JA55TF83MR

ISNAD

Sevinç, Selçuk - Halıcı, Mehmet Gökhan - Cumaoğlu, Ahmet. “Screening of Antimicrobial and Antiinflammatory Activities of Three Lichenized Fungal Extracts Collected from Northwest Anatolia (Türkiye)”. Journal of Research in Pharmacy 28/4 (July 1, 2025): 1166-1173. https://izlik.org/JA55TF83MR.

JAMA

1.Sevinç S, Halıcı MG, Cumaoğlu A. Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye). J. Res. Pharm. 2025;28:1166–1173.

MLA

Sevinç, Selçuk, et al. “Screening of Antimicrobial and Antiinflammatory Activities of Three Lichenized Fungal Extracts Collected from Northwest Anatolia (Türkiye)”. Journal of Research in Pharmacy, vol. 28, no. 4, July 2025, pp. 1166-73, https://izlik.org/JA55TF83MR.

Vancouver

1.Selçuk Sevinç, Mehmet Gökhan Halıcı, Ahmet Cumaoğlu. Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye). J. Res. Pharm. [Internet]. 2025 Jul. 1;28(4):1166-73. Available from: https://izlik.org/JA55TF83MR