In vitro bioavailability studies on phytochemical profile and antioxidant activity potential of Clematis viticella L.

Esra Acar-şah; Engin Celep; Erdem Yeşilada

In vitro bioavailability studies on phytochemical profile and antioxidant activity potential of Clematis viticella L.

Abstract

Since biotransformation processes generally affect the biological activities of phytochemical compounds, bioavailability is one of the primary steps in determining the potency of the activity. Previous studies have shown that in vitro antioxidant capacity of phytochemicals cannot completely reflect real health benefits. Clematis species (Ranunculaceae) are used to eliminate the symptoms of many diseases including particularly in the treatment of rheumatic diseases in Turkey, however the number of studies conducted is limited. In the light of this information, the total phytochemical profile of the extract of aerial parts of C. viticella prepared with 80% methanol and the gastrointestinal digestion products and the antioxidant activity profile were evaluated by subjecting them to the in vitro simulation model of the human digestive tract in the study. It was observed that both total phytochemical content and antioxidant activity decreased after simulated human digestion. Moreover, this study was shown potential bioaccessibility and bioavailability of total phenol, phenolic acid, flavonoid, and saponin content found in the aerial parts of Clematis viticella were reduced. Consequently, these indicate that it can result from the complicated interaction of compounds in the plant matrix in an in vitro simulated human digestion.

Keywords

References

[1] Baytop T. Türkiye’de Bitkiler İle Tedavi, Geçmişte ve Bugün. İstanbul: Nobel Tıp; 1999.
[2] Küpeli E. Clematis vitalba L. bitkisinin romatizma tedavisindeki etkisi üzerinde çalışmalar [PhD Thesis]. Ankara: Gazi Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı; 2004.
[3] Davis PH. Clematis. In: Davis PH, editor. Flora of Turkey and the East Aegean Islands. Edinburgh: Edinburgh University Press; 1984. p.138–140.
[4] Chawla R, Kumar S, Sharma A. The genus Clematis (Ranunculaceae): chemical and pharmacological perspectives. J Ethnopharmacol. 2012;143:116–150. [CrossRef]
[5] Yesilada E, Küpeli E. Clematis vitalba L. aerial part exhibits potent anti-inflammatory, antinociceptive and antipyretic effects. J Ethnopharmacol. 2007;110:504–515. [CrossRef]
[6] Kyung KH, Woo Y, Kim DS, Park HJ, Kim YS. Antimicrobial activity of an edible wild plant, Apiifolia virgin’s bower (Clematis apiifolia DC). Food Sci Biotechnol. 2007;16:1051–1054.
[7] Hung TM, Thuong PT, Bae K. Antioxidant effect of flavonoids isolated from the root of Clematis trichotoma Nakai. Korean J Med Crop Sci. 2005;13(5):227–232.
[8] Peng H, Lv H, Wang Y, Liu YH, Li CY, Meng L, Chen F, Bao JK. Clematis montana lectin, a novel mannose-binding lectin from traditional Chinese medicine with antiviral and apoptosis-inducing activities. Peptides. 2009;30:1805–1815. [CrossRef]

[9] Álvarez ME, María AOM, Villegas O, Saad JR. Evaluation of diuretic activity of the constituents of Clematis montevidensis Spreng. (Ranunculaceae) in rats. Phytother Res. 2003;17:958–960. [CrossRef]
[10] Ho CS, Wong YH, Chiu KW. The hypotensive action of Desmodium styracifolium and Clematis chinensis. Am J Chin Med. 1989;17(3–4):189–202. [CrossRef]
[11] Marrelli M, Loizzo MR, Nicoletti M, Menichini F, Conforti F. Inhibition of key enzymes linked to obesity by preparations from Mediterranean dietary plants: effects on α-amylase and pancreatic lipase activities. Plant Foods Hum Nutr. 2013;68:340–346.
[12] Hur SJ, Lim BO, Decker EA, McClements DJ. In vitro human digestion models for food applications. Food Chem. 2011;125:1–12. [CrossRef]
[13] Alminger M, Aura AM, Bohn T, et al. In vitro models for studying secondary plant metabolite digestion and bioaccessibility. Compr Rev Food Sci Food Saf. 2014;13:413–436. [CrossRef]
[14] Celep E, Akyüz S, İnan Y, Yesilada E. Assessment of potential bioavailability of major phenolic compounds in Lavandula stoechas L. ssp. stoechas. Ind Crops Prod. 2018;118:111–117. [CrossRef]
[15] Floyd RA. Role of oxygen free radicals in carcinogenesis and brain ischemia. FASEB J. 1990;4(9):2587–2597. [CrossRef]
[16] Koşar M, Göger F, Baser KHC. In vitro antioxidant properties and phenolic composition of Salvia virgata Jacq. from Turkey. J Agric Food Chem. 2008;56:2369–2374.
[17] Rice-Evans C, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997;2(4):152–159. [CrossRef]
[18] Villanueva-Carvajal A, et al. In vitro gastrointestinal digestion of Hibiscus sabdariffa L.: the use of its natural matrix to improve the concentration of phenolic compounds in gut. LWT - Food Sci Technol. 2013;51:260–265. [CrossRef]
[19] Celep E, Charehsaz M, Akyüz S, Acar ET, Yesilada E. Effect of in vitro gastrointestinal digestion on the bioavailability of phenolic components and the antioxidant potentials of some Turkish fruit wines. Food Res Int. 2015;78:209–215. [CrossRef]
[20] McDougall GJ, Fyffe S, Dobson P, Stewart D. Anthocyanins from red wine - their stability under simulated gastrointestinal digestion. Phytochemistry. 2005;66:2540–2548. [CrossRef]
[21] Kırmızıbekmez H, İnan Y, Reis R, Sipahi H, Gören AC, Yesilada E. Phenolic compounds from the aerial parts of Clematis viticella L. and their in vitro anti-inflammatory activities. Nat Prod Res. 2018:1–4. [CrossRef]
[22] Kim EO, et al. Bioavailability of ginsenosides from white and red ginseng in the simulated digestion model. J Agric Food Chem. 2014;62(41):10055–10063.
[23] Bouayed J, Hoffmann L, Bohn T. Total phenolics, flavonoids, anthocyanins and antioxidant activity following simulated gastrointestinal digestion and dialysis of apple varieties: bioaccessibility and potential uptake. Food Chem. 2011;128:14–21. [CrossRef]
[24] Siracusa L, et al. Phenolic composition and antioxidant activity of aqueous infusions from Capparis spinosa L. and Crithmum maritimum L. before and after submission to a two-step in vitro digestion model. J Agric Food Chem. 2011;59(23):12453–12459.
[25] Wong YH, Tan CP, Long K, Nyam KL. In vitro simulated digestion on the biostability of Hibiscus cannabinus L. seed extract. Czech J Food Sci. 2014;32(2):177–181.
[26] Celep E, İnan Y, Akyüz S, Yesilada E. The bioaccessible phenolic profile and antioxidant potential of Hypericum perfoliatum L. after simulated human digestion. Ind Crops Prod. 2017;109:717–723. [CrossRef]
[27] Kirby AJ, Schmidt RJ. The antioxidant activity of Chinese herbs for eczema and of placebo herbs - I. J Ethnopharmacol. 1997;56:103–108. [CrossRef]
[28] Gan RY, et al. Screening of natural antioxidants from traditional Chinese medicinal plants associated with treatment of rheumatic disease. Molecules. 2010;15:5988–5997. [CrossRef]
[29] Atmani D, et al. Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem. 2009;112:303–309. [CrossRef]
[30] Du ZZ, et al. A new flavone C-glycoside from Clematis rehderiana. Molecules. 2010;15:672–679. [CrossRef]
[31] Pandanaboina SC, et al. Alterations in antioxidant enzyme activities and oxidative damage in alcoholic rat tissues: Protective role of Thespesia populnea. Food Chem. 2012;132:150–159. [CrossRef]
[32] Henning SM, et al. Variability in the antioxidant activity of dietary supplements from pomegranate, milk thistle, green tea, grape seed, goji, and acai: effects of in vitro digestion. J Agric Food Chem. 2014;62:4313–4321. [CrossRef]
[33] Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acids reagents. Am J Enol Viticult. 1965;16:144–158.
[34] Mihailović V, et al. Chemical profile, antioxidant activity and stability in stimulated gastrointestinal tract model system of three Verbascum species. Ind Crops Prod. 2016;89:141–151. [CrossRef]
[35] Woisky RG, Salatino A. Analysis of propolis: some parameters and procedures for chemical quality control. J Apic Res. 1998;37(2):99–105. [CrossRef]
[36] Ariffin F, et al. Antioxidant capacity and phenolic composition of fermented Centella asiatica herbal teas. J Sci Food Agric. 2011;91:2731–2739. [CrossRef]
[37] Hiai S, Oura H, Nakajima T. Color reaction of some sapogenins and saponins with vanillin and sulfuric acid. Planta Med. 1976;29:116–122.
[38] Gayoso L, et al. Bioaccessibility of rutin, caffeic acid and rosmarinic acid: influence of the in vitro gastrointestinal digestion models. J Funct Foods. 2016;26:428–438. [CrossRef]
[39] Akter MS, Ahmed M, Eun JB. Solvent effects on antioxidant properties of persimmon (Diospyros kaki L. cv. Daebong) seeds. Int J Food Sci Technol. 2010;45:2258–2264. [CrossRef]
[40] Celep E, Aydin A, Yesilada E. A comparative study on the in vitro antioxidant potentials of three edible fruits: Cornelian cherry, Japanese persimmon and cherry laurel. Food Chem Toxicol. 2012;50:3329–3335. [CrossRef]
[41] Fogliano V, et al. Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J Agric Food Chem. 1999;47:1035–1040.
[42] Benzie IFF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a measure of “Antioxidant Power”: the FRAP assay. Anal Biochem. 1996;239:70–76. [CrossRef]
[43] Apak R, Güçlü K, Özyürek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem. 2004;52:7970–7981.
[44] Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdinum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269:337–341. [CrossRef]

Details

Primary Language

English

Subjects

Pharmacognosy, Pharmaceutical Botany, Clinical Pharmacology and Therapeutics

Journal Section

Research Article

Authors

Esra Acar-şah This is me
Türkiye

Engin Celep ^*
Türkiye

Erdem Yeşilada
Türkiye

Publication Date

June 27, 2025

Submission Date

September 17, 2018

Acceptance Date

December 19, 2018

Published in Issue

Year 2019 Volume: 23 Number: 2

IZ

https://izlik.org/JA62JS56XY

Cite

RIS / Bibtex

APA

Acar-şah, E., Celep, E., & Yeşilada, E. (2025). In vitro bioavailability studies on phytochemical profile and antioxidant activity potential of Clematis viticella L. Journal of Research in Pharmacy, 23(2), 326-335. https://izlik.org/JA62JS56XY

AMA

1.Acar-şah E, Celep E, Yeşilada E. In vitro bioavailability studies on phytochemical profile and antioxidant activity potential of Clematis viticella L. J. Res. Pharm. 2025;23(2):326-335. https://izlik.org/JA62JS56XY

Chicago

Acar-şah, Esra, Engin Celep, and Erdem Yeşilada. 2025. “In Vitro Bioavailability Studies on Phytochemical Profile and Antioxidant Activity Potential of Clematis Viticella L”. Journal of Research in Pharmacy 23 (2): 326-35. https://izlik.org/JA62JS56XY.

EndNote

Acar-şah E, Celep E, Yeşilada E (June 1, 2025) In vitro bioavailability studies on phytochemical profile and antioxidant activity potential of Clematis viticella L. Journal of Research in Pharmacy 23 2 326–335.

IEEE

[1]E. Acar-şah, E. Celep, and E. Yeşilada, “In vitro bioavailability studies on phytochemical profile and antioxidant activity potential of Clematis viticella L”., J. Res. Pharm., vol. 23, no. 2, pp. 326–335, June 2025, [Online]. Available: https://izlik.org/JA62JS56XY

ISNAD

Acar-şah, Esra - Celep, Engin - Yeşilada, Erdem. “In Vitro Bioavailability Studies on Phytochemical Profile and Antioxidant Activity Potential of Clematis Viticella L”. Journal of Research in Pharmacy 23/2 (June 1, 2025): 326-335. https://izlik.org/JA62JS56XY.

JAMA

1.Acar-şah E, Celep E, Yeşilada E. In vitro bioavailability studies on phytochemical profile and antioxidant activity potential of Clematis viticella L. J. Res. Pharm. 2025;23:326–335.

MLA

Acar-şah, Esra, et al. “In Vitro Bioavailability Studies on Phytochemical Profile and Antioxidant Activity Potential of Clematis Viticella L”. Journal of Research in Pharmacy, vol. 23, no. 2, June 2025, pp. 326-35, https://izlik.org/JA62JS56XY.

Vancouver

1.Esra Acar-şah, Engin Celep, Erdem Yeşilada. In vitro bioavailability studies on phytochemical profile and antioxidant activity potential of Clematis viticella L. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(2):326-35. Available from: https://izlik.org/JA62JS56XY