Therapeutic properties of oyster mushroom (P. ostreatus) cultivation on different cellulosic substrates

Mehmet Akyüz; Şule İnci; Sevda Kırbağ

Therapeutic properties of oyster mushroom (P. ostreatus) cultivation on different cellulosic substrates

Abstract

The objectives of this work were to evaluate the antimicrobial activities, antioxidant in vitro cytotoxicity and DNA damage of Pleurotus ostreatus (Jacq.) P. Kumm. cultivation on some local cellulosic substrates. It observed to be very active against bacteria (except S. mutans and K. pneumonia), yeast and dermatophytes when compared to the control groups (10.0-32.7 mm). It also showed significant antioxidant potential, the highest TAS (0.978 mmol/L) and TOS (10.51 µmol/L) values were obtained in M-P (1:1) and W, and also DPPH radical scavenging effects was more effective at 25 mg (74.80%) on MS. The methanol extract of P. ostreatus cultured on M-W (2.35%) and M-P (1:1) (9.74%) at 400 μg/mL concentration has remarkably decreased the percentage of viability in MDA-MB-231 and A-549 cell lines. However, it has been determined that P. ostreatus does not have a DNA protective effect. These results indicate that P. ostreatus has potent antimicrobial, antioxidant, as well as cytotoxic effects.

Keywords

References

[1] Cerutti PA. Oxidant stress and carcinogenesis. Eur J Clin Investig. 1991; 21(1): 1-5. [CrossRef]
[2] Duthie DD. Lipid peroxidation. Eur J Clin Investig. 1993; 47(11): 759-764.
[3] Pietta PG. Flavonoids as antioxidants. J Nat Prod. 2000; 63(7): 1035-1042. [CrossRef]
[4] Adebayo EA, Martínez‐Carrera D, Morales P, Sobal M, Escudero H, Meneses ME, Avila-Nava A, Castillo I, Bonilla M. Comparative study of antioxidant and antibacterial properties of the edible mushrooms Pleurotus levis, P. ostreatus, P. pulmonarius and P. tuber‐regium. Int J Food Sci Technol. 2018; 53(5): 1316-1330. [CrossRef]
[5] Gregori A, Švagelj M, Pohleven J. Cultivation techniques and medicinal properties of Pleurotus spp. Food Technol Biotechnol. 2007; 45(3): 238-249.
[6] Abidin MHZ, Abdullah N, Abidin NZ. Therapeutic properties of Pleurotus species (oyster mushrooms) for atherosclerosis: A review. Int J Food Prop. 2017; 20(6): 1251-1261. [CrossRef]
[7] Venturella G, Ferraro V, Cirlincione F, Gargano ML. Medicinal mushrooms: bioactive compounds, use and clinical trials. Int J Mol Sci. 2021; 22(2): 634-664. [CrossRef]
[8] Melanouri EM, Dedousi M, Diamantopoulou P. Cultivating Pleurotus ostreatus and Pleurotus eryngii mushroom strains on agro-industrial residues in solid-state fermentation. Part II: Effect on productivity and quality of carposomes. Carbon Resour Convers. 2022; 5: 61–70. [CrossRef]

[9] Bellettini MB, Fiorda FA, Maieves HA, Teixeira GL, Avila S, Hornung PS, Junior AM, Ribani RH. Factors affecting mushroom Pleurotus spp. Saudi J Biol Sci. 2019; 26 (4): 633-646. [CrossRef]
[10] Pandey AT, Pandey I, Kerkar P, Singh MP. Antimicrobial activity and mycochemical profile of methanol extract from Pleurotus flabellatus. Vegetos, 2021; 34(3): 619-629. [CrossRef]
[11] Younis AM, Wu FS, El Shikh HH. Antimicrobial activity of extracts of the oyster culinary medicinal mushroom Pleurotus ostreatus (higher basidiomycetes) and identification of a new antimicrobial compound. Int J Med Mushrooms. 2015; 17(6): 57-90. [CrossRef]
[12] Gashaw G, Fassil A, Redi F. Evaluation of the antibacterial activity of Pleurotus spp. cultivated on different agricultural wastes in Chiro, Ethiopia. Int J Microbiol. 2020; 9312489: 1-9. [CrossRef]
[13] Schillaci D, Arizza V, Gargano ML, Venturella G. Antibacterial activity of Mediterranean oyster mushrooms, species of genus Pleurotus (higher basidiomycetes). Int J Med Mushrooms. 2013; 15(6): 591-594. [CrossRef]
[14] Gürgen A, Sevindik M, Yildiz S, Akgül H. Determination of antioxidant and oxidant potentials of Pleurotus citrinopileatus mushroom cultivated on various substrates. KSU J Agric Nat. 2020; 23: 586-591. [CrossRef]
[15] Yildirim NC, Turkoglu S, Yildirim N, Kaplan IO. Antioxidant properties of wild edible mushroom Pleurotus eryngii collected from Tunceli province of Turkey. Dig J Nanomater Biostruct. 2012; 7(4): 1647–1654.
[16] Güzel EE, Tektemur, NK, Tektemur A, Acay H, Yildirim A. The antioxidant and anti‐apoptotic potential of Pleurotus eryngii extract and its chitosan‐loaded nanoparticles against doxorubicin‐induced testicular toxicity in male rats. Andrologia. 2021; 53(11): e14225. [CrossRef]
[17] Yıldız S, Gürgen A, Sevindik M. Antioxidant and antimicrobial activities of Pleurotus djamor cultivated in various composts. In: II. International GAP Conference on Mathematics- Engineering- Science and Medical Studies. 2019 June 21–23; Adıyaman. Turkey: 2019. p 279–286.
[18] Sevindik M, Celal B, Akgül H. Comparison of antioxidant potentials of the wild and cultivated forms of edible Pleurotus ostreatus and Agaricus bisporus mushrooms. Turk J Life Sci 2018; 3(2): 263-266.
[19] Akyüz M, Kireçci ADÖ, Gökçe Z, Kirbağ S, Yilmaz Ö. Biochemical constituents and antioxidant activities of some mushrooms from Turkey: Agaricus spp., Pleurotus spp., Morchella esculenta and Terfezia boudieri. İstanbul J Pharm. 2019; 49(1): 1-6. [CrossRef]
[20] Elhusseiny SM, El-Mahdy TS, Awad MF, Elleboudy NS, Farag M, Aboshanab KM, Yassien MA. Antiviral, cytotoxic, and antioxidant activities of three edible Agaricomycetes mushrooms: Pleurotus columbinus, Pleurotus sajor-caju and Agaricus bisporus. J Fungi. 2021; 7(8): 645. [CrossRef]
[21] Choi D, Cha WS, Kang SH, Lee BR. Effect of Pleurotus ferulae extracts on viability of human lung cancer and cervical cancer cell lines. Biotechnol Bioprocess Eng. 2004; 9(5): 356-361. [CrossRef]
[22] Choi HY, Ryu HS. Antioxidant and anticancer effects of water extract from Pleurotus ostreatus. Korean J Food Nutr. 2015; 28(1): 60-65. [CrossRef]
[23] Wu X, Zheng S, Cui, L, Wang H, Ng TB. Isolation and characterization of a novel ribonuclease from the pink oyster mushroom Pleurotus djamor. J Gen Appl Microbiol. 2010; 56: 231-239. [CrossRef]
[24] Cui H, Wang C, Wang Y, Li Z, Zhang Y, Chen M, Li F. Pleurotus nebrodensis polysaccharide induces apoptosis in human non-small cell lung cancer A549 cells. Carbohydr Polym. 2014; 104(1): 246-252. [CrossRef]
[25] Ma G, Yang W, Mariga AM, Fang Y, Ma N, Pei F, Hu Q. Purification, characterization and antitumor activity of polysaccharides from Pleurotus eryngii residue. Carbohydr Polym. 2014; 114: 297-305. [CrossRef]
[26] Mariga AM, Pei F, Yang WJ, Zhao LY, Shao YN, Mugambi DK, Hu Q. Immunopotentiation of Pleurotus eryngii (DC. ex Fr.) Quel. J Ethnopharmacol. 2014; 153(3): 604-614. [CrossRef]
[27] Raman J, Reddy GR, Lakshmanan H, Selvaraj V, Gajendran B, Nanjian R, Chinnasamy A, Sabaratnam V. Mycosynthesis and characterization of silver nanoparticles from Pleurotus djamor var. roseus and their in vitro cytotoxicity effect on PC3 cells. Process Biochem. 2015; 50(1): 140-147. [CrossRef]
[28] El-Deeb NM, El-Adawi HI, EL-wahab AEA, Haddad AM, El Enshasy HA, He YW, Davis KR. Modulation of NKG2D, KIR2DL and cytokine production by Pleurotus ostreatus glucan enhances natural killer cell cytotoxicity toward cancer cells. Front Cell Dev Biol. 2019; 7: 165. [CrossRef]
[29] Uddin Pk MM, Islam MS, Pervin R, Dutta S, Talukder RI, Rahman M. Optimization of extraction of antioxidant polysaccharide from Pleurotus ostreatus (Jacq.) P. Kumm and its cytotoxic activity against murine lymphoid cancer cell line. PloS One. 2019; 14(1): e0209371. [CrossRef]
[30] Nallathamby N, Lakshmanan H, Vidyadaran S, Malek SNA, Phan CW, Sabaratnam V. Lipid-rich fraction of the sclerotium of tiger milk mushroom Lignosus rhinocerotis (Agaricomycetes) attenuates LPS-induced inflammation in BV2 cells via Nrf2 pathway. Braz J Pharm Sci. 2020; 56: e18499. [CrossRef]
[31] Kim KC, Kim IG. Ganoderma lucidum extract protects DNA from strand breakage caused by hydroxyl radical and UV irradiation. Int J Mol Med. 1999; 4: 273-277. [CrossRef]
[32] Pillai TG, Salvi VP, Maurya DK, Nair CKK, Janardhanan KK. Prevention of radiation-induced damages by aqueous extract of Ganoderma lucidum occurring in southern parts of India. Curr Sci. 2006; 91: 341-344.
[33] Zhao L, Zhao G, Hui B, Mao Z, Tong J, Hu X. Effect of selenium on increasing the antioxidant activity of protein extracts from a selenium-enriched mushroom species of the Ganoderma genus. J Food Sci. 2004; 69: 184-188. [CrossRef]
[34] Zhao L, Zhao, GH, Du M, Zhao ZD, Xiao LX, Hu XS. Effect of selenium on increasing free radical scavenging activities of polysaccharide extracts from a Se-enriched mushroom species of the genus Ganoderma. Eur Food Res Technol. 2008; 226: 499-505. [CrossRef]
[35] Akyüz M, Karagöz ID, Inci S, Kilic IH, Kirbag S, Simitcioglu B. Cytotoxicity and DNA protective effects of the Terfezia and Picoa species from the eastern region of Turkey. Istanbul J Pharm. 2021; 51(2): 198-204. [CrossRef]
[36] Akyüz M, İnci Ş, Kırbağ S. Nutritive value of Pleurotus ostreatus (Jacq.) P. Kumm. grown on some cellulosic residues. Artvin Çoruh Univ J For Fac. 2021; 22(2): 218-221. [CrossRef]
[37] Collins CM, Lyne PM. Microbiological methods. In: Collins CH, Lyne PM, Grange JM, Flkinham III JO, editors. Mycological methods. 8 ed. London: 2004. p.140.
[38] European Committee for Antimicrobial Susceptibility Testing (EUCAST). Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution. Clin Microbiol Infect Dis. 2003; 9: 17. [CrossRef]
[39] Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochem. 2004;37(4):277-85. [CrossRef]
[40] Erel O. A new automated colorimetric method for measuring total oxidant status. Clinical Biochem. 2005; 38(12): 1103-11. [CrossRef]
[41] Cuendet M, Hostettmann K, Potterat O, Dyatmiko W. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helv Chim Acta. 1997; 80(4): 1144-1152. [CrossRef]
[42] Kirby AJ, Schmidt RJ. The antioxidant activity of Chinese herbs for eczema and of placebo herbs. J Ethnopharmacol. 1997; 56(2): 103-108. [CrossRef]
[43] Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65: 55-63. [CrossRef]
[44] Russo A, Acquaviva R, Campisi A, Sorrenti V, Di-Giacomo C, Virgata G, Barcellona ML, Vanella A. Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectors. Cell Bio Toxico. 2000; 16: 91-98. [CrossRef]
[45] Tepe B, Degerli S, Arslan S, Malatyali E, Sarikurkcu C. Determination of chemical profile, antioxidant, DNA damage protection and antiamobic activities of Teucrium polium and Stachy siberica. Fitoterapia. 2011; 82: 237-246. [CrossRef]

Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Mehmet Akyüz ^*
0000-0003-3986-3498
Türkiye

Şule İnci
0000-0002-4022-5269
Türkiye

Sevda Kırbağ
0000-0002-4337-8236
Türkiye

Publication Date

June 28, 2025

Submission Date

April 15, 2022

Acceptance Date

May 14, 2022

Published in Issue

Year 2023 Volume: 27 Number: 1

IZ

https://izlik.org/JA76ZB85PW

Cite

RIS / Bibtex

APA

Akyüz, M., İnci, Ş., & Kırbağ, S. (2025). Therapeutic properties of oyster mushroom (P. ostreatus) cultivation on different cellulosic substrates. Journal of Research in Pharmacy, 27(1), 320-328. https://izlik.org/JA76ZB85PW

AMA

1.Akyüz M, İnci Ş, Kırbağ S. Therapeutic properties of oyster mushroom (P. ostreatus) cultivation on different cellulosic substrates. J. Res. Pharm. 2025;27(1):320-328. https://izlik.org/JA76ZB85PW

Chicago

Akyüz, Mehmet, Şule İnci, and Sevda Kırbağ. 2025. “Therapeutic Properties of Oyster Mushroom (P. Ostreatus) Cultivation on Different Cellulosic Substrates”. Journal of Research in Pharmacy 27 (1): 320-28. https://izlik.org/JA76ZB85PW.

EndNote

Akyüz M, İnci Ş, Kırbağ S (June 1, 2025) Therapeutic properties of oyster mushroom (P. ostreatus) cultivation on different cellulosic substrates. Journal of Research in Pharmacy 27 1 320–328.

IEEE

[1]M. Akyüz, Ş. İnci, and S. Kırbağ, “Therapeutic properties of oyster mushroom (P. ostreatus) cultivation on different cellulosic substrates”, J. Res. Pharm., vol. 27, no. 1, pp. 320–328, June 2025, [Online]. Available: https://izlik.org/JA76ZB85PW

ISNAD

Akyüz, Mehmet - İnci, Şule - Kırbağ, Sevda. “Therapeutic Properties of Oyster Mushroom (P. Ostreatus) Cultivation on Different Cellulosic Substrates”. Journal of Research in Pharmacy 27/1 (June 1, 2025): 320-328. https://izlik.org/JA76ZB85PW.

JAMA

1.Akyüz M, İnci Ş, Kırbağ S. Therapeutic properties of oyster mushroom (P. ostreatus) cultivation on different cellulosic substrates. J. Res. Pharm. 2025;27:320–328.

MLA

Akyüz, Mehmet, et al. “Therapeutic Properties of Oyster Mushroom (P. Ostreatus) Cultivation on Different Cellulosic Substrates”. Journal of Research in Pharmacy, vol. 27, no. 1, June 2025, pp. 320-8, https://izlik.org/JA76ZB85PW.

Vancouver

1.Mehmet Akyüz, Şule İnci, Sevda Kırbağ. Therapeutic properties of oyster mushroom (P. ostreatus) cultivation on different cellulosic substrates. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(1):320-8. Available from: https://izlik.org/JA76ZB85PW