The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression

Betül Ayaz Adakul; Büşra Ertaş; Zatiye Ayça Çevikelli; Dilek Özbeyli; Feriha Ercan; Cansu Kandemir; Ozge Cevik; Tarık Şener; Göksel Şener

The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression

Abstract

Reactive oxygen metabolites play important roles in ischemia/reperfusion (I/R) injury in several organ systems. Riboflavin has been shown to exert antioxidant and/or anti-inflammatory activities in several experimental models. The aim of this study was to investigate the role of riboflavin against I/R injury in the rat kidney. Wistar albino rats 200-300 g weighing were divided into 3 groups. One week after unilateral nephrectomy, the IR procedure was applied to the rats. To induce I/R injury renal pedicle was clamped for 45 minutes and then rats were allowed reperfusion for 6 hours. Riboflavin (25 mg/kg, orally) or vehicle was administered for one week as pretreatment. After decapitation, kidney tissue samples were taken for the evaluation of malondialdehyde (MDA), an end product of lipid peroxidation; glutathione (GSH), a key antioxidant; and 8-hydroxydeoxyguanosine (8-OHdG), a specific marker of oxidative DNA damage. Furthermore, myeloperoxidase (MPO) and caspase-3 activities were also examined together with histological analysis. Ischemia/reperfusion induced significant increases in MDA and 8-OHdG levels and MPO and caspase- 3 activities, and decrese in GSH levels. In the riboflavin treatment these indices were found to be reversed back to control levels. The present data demonstrated that riboflavin, through its antioxidant effect, attenuates I/R induced acute renal injury in rats.

Keywords

References

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Betül Ayaz Adakul This is me
Türkiye

Büşra Ertaş
Türkiye

Zatiye Ayça Çevikelli This is me
Türkiye

Dilek Özbeyli
Türkiye

Feriha Ercan This is me
Türkiye

Cansu Kandemir
Türkiye

Ozge Cevik
Türkiye

Tarık Şener
Türkiye

Göksel Şener ^*
Türkiye

Publication Date

June 27, 2025

Submission Date

December 17, 2018

Acceptance Date

January 25, 2019

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA59LS69FR

Cite

RIS / Bibtex

APA

Ayaz Adakul, B., Ertaş, B., Çevikelli, Z. A., Özbeyli, D., Ercan, F., Kandemir, C., Cevik, O., Şener, T., & Şener, G. (2025). The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression. Journal of Research in Pharmacy, 23(3), 379-386. https://izlik.org/JA59LS69FR

AMA

1.Ayaz Adakul B, Ertaş B, Çevikelli ZA, et al. The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression. J. Res. Pharm. 2025;23(3):379-386. https://izlik.org/JA59LS69FR

Chicago

Ayaz Adakul, Betül, Büşra Ertaş, Zatiye Ayça Çevikelli, et al. 2025. “The Effects of Riboflavin on Ischemia Reperfusion Induced Renal Injury: Role on Caspase-3 Expression”. Journal of Research in Pharmacy 23 (3): 379-86. https://izlik.org/JA59LS69FR.

EndNote

Ayaz Adakul B, Ertaş B, Çevikelli ZA, Özbeyli D, Ercan F, Kandemir C, Cevik O, Şener T, Şener G (June 1, 2025) The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression. Journal of Research in Pharmacy 23 3 379–386.

IEEE

[1]B. Ayaz Adakul et al., “The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression”, J. Res. Pharm., vol. 23, no. 3, pp. 379–386, June 2025, [Online]. Available: https://izlik.org/JA59LS69FR

ISNAD

Ayaz Adakul, Betül - Ertaş, Büşra - Çevikelli, Zatiye Ayça - Özbeyli, Dilek - Ercan, Feriha - Kandemir, Cansu - Cevik, Ozge - Şener, Tarık - Şener, Göksel. “The Effects of Riboflavin on Ischemia Reperfusion Induced Renal Injury: Role on Caspase-3 Expression”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 379-386. https://izlik.org/JA59LS69FR.

JAMA

1.Ayaz Adakul B, Ertaş B, Çevikelli ZA, Özbeyli D, Ercan F, Kandemir C, Cevik O, Şener T, Şener G. The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression. J. Res. Pharm. 2025;23:379–386.

MLA

Ayaz Adakul, Betül, et al. “The Effects of Riboflavin on Ischemia Reperfusion Induced Renal Injury: Role on Caspase-3 Expression”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 379-86, https://izlik.org/JA59LS69FR.

Vancouver

1.Betül Ayaz Adakul, Büşra Ertaş, Zatiye Ayça Çevikelli, Dilek Özbeyli, Feriha Ercan, Cansu Kandemir, Ozge Cevik, Tarık Şener, Göksel Şener. The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):379-86. Available from: https://izlik.org/JA59LS69FR