Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens

Merve Eylül Kıymacı; Merve Şavluk; Mehmet Gümüştaş; Mehmet Üvey; Nilgün Ünal

doi:10.29228/jrp.333

Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens

Abstract

The development of increasing resistance to antibiotics such as colistin, problems arise in the treatment of bacterial infections and make it necessary to search new alternative methods. For this purpose, plant-based approaches are among the important research topics depending on their traditional uses. The aim of the present study was to investigate the antibacterial and antibiofilm activity of tea tree oil purchased from a local market against a variety of 66 colistin resistant Salmonella enterica serotypes isolated from poultry farm environmental samples. Content analysis of TTO was determined by gas chromatography/mass spectroscopy. The antibacterial activity was determined by broth microdilution method, and antibiofilm activity was examined by crystal violet method. As a result, terpinen-4-ol was found as major component of TTO with 35.9% ratio. The MIC values of TTO were differed between 6250-12500 µg/mL. 27 of 66 isolates formed biofilm and 25 of 27 isolates belonged to S. Infantis. The biofilm reduction of TTO at sub- inhibitory concentration were found between 52-84.4%. Current study should be supported by future studies to determine the effectiveness of TTO to be among the agents that can be used together with antimicrobials in the attenuation of microorganisms.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Microbiology

Journal Section

Research Article

Authors

Merve Eylül Kıymacı
Türkiye

Merve Şavluk
0000-0003-0740-799X
Türkiye

Mehmet Gümüştaş
0000-0003-2793-7154
Türkiye

Mehmet Üvey
0000-0002-2615-2673
Türkiye

Nilgün Ünal ^*
0000-0002-0490-976X
Türkiye

Publication Date

June 27, 2025

Submission Date

November 2, 2022

Acceptance Date

January 3, 2023

Published in Issue

Year 2023 Volume: 27 Number: 2

DOI

https://doi.org/10.29228/jrp.333

IZ

https://izlik.org/JA78MM65JM

Cite

RIS / Bibtex

APA

Kıymacı, M. E., Şavluk, M., Gümüştaş, M., Üvey, M., & Ünal, N. (2025). Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens. Journal of Research in Pharmacy, 27(2), 508-518. https://doi.org/10.29228/jrp.333

AMA

1.Kıymacı ME, Şavluk M, Gümüştaş M, Üvey M, Ünal N. Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens. J. Res. Pharm. 2025;27(2):508-518. doi:10.29228/jrp.333

Chicago

Kıymacı, Merve Eylül, Merve Şavluk, Mehmet Gümüştaş, Mehmet Üvey, and Nilgün Ünal. 2025. “Antibacterial and Antibiofilm Activity of Melaleuca Alternifolia (tea Tree) Essential Oil Against Colistin Resistant Salmonella Enterica Serotypes Isolated from Poultry Environmental Specimens”. Journal of Research in Pharmacy 27 (2): 508-18. https://doi.org/10.29228/jrp.333.

EndNote

Kıymacı ME, Şavluk M, Gümüştaş M, Üvey M, Ünal N (June 1, 2025) Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens. Journal of Research in Pharmacy 27 2 508–518.

IEEE

[1]M. E. Kıymacı, M. Şavluk, M. Gümüştaş, M. Üvey, and N. Ünal, “Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens”, J. Res. Pharm., vol. 27, no. 2, pp. 508–518, June 2025, doi: 10.29228/jrp.333.

ISNAD

Kıymacı, Merve Eylül - Şavluk, Merve - Gümüştaş, Mehmet - Üvey, Mehmet - Ünal, Nilgün. “Antibacterial and Antibiofilm Activity of Melaleuca Alternifolia (tea Tree) Essential Oil Against Colistin Resistant Salmonella Enterica Serotypes Isolated from Poultry Environmental Specimens”. Journal of Research in Pharmacy 27/2 (June 1, 2025): 508-518. https://doi.org/10.29228/jrp.333.

JAMA

1.Kıymacı ME, Şavluk M, Gümüştaş M, Üvey M, Ünal N. Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens. J. Res. Pharm. 2025;27:508–518.

MLA

Kıymacı, Merve Eylül, et al. “Antibacterial and Antibiofilm Activity of Melaleuca Alternifolia (tea Tree) Essential Oil Against Colistin Resistant Salmonella Enterica Serotypes Isolated from Poultry Environmental Specimens”. Journal of Research in Pharmacy, vol. 27, no. 2, June 2025, pp. 508-1, doi:10.29228/jrp.333.

Vancouver

1.Merve Eylül Kıymacı, Merve Şavluk, Mehmet Gümüştaş, Mehmet Üvey, Nilgün Ünal. Antibacterial and antibiofilm activity of Melaleuca alternifolia (tea tree) essential oil against colistin resistant Salmonella enterica serotypes isolated from poultry environmental specimens. J. Res. Pharm. 2025 Jun. 1;27(2):508-1. doi:10.29228/jrp.333