Klotho gene polymorphism as a susceptibility factor for oxidative DNA damage in coronary artery disease

Seher Karslı; Selma Yazar; Mehmet Karaca; Türkan Yurdun

Klotho gene polymorphism as a susceptibility factor for oxidative DNA damage in coronary artery disease

Abstract

The Klotho protein has been linked to the promotion of cardiovascular health maintenance through its capacity to enhance resistance against oxidative stress, thereby exerting protective effects. The potential involvement of Klotho gene polymorphisms in the regulation of human aging and age-related disorders, including coronary artery disease, chronic renal disease, osteoporosis, and stroke, is under investigation. The objective of our research was to investigate the association between the rs9527025 (Cys370Ser) polymorphism of the Klotho gene and coronary artery calcification, as well as the potential link between the rs9527025 polymorphism and oxidative DNA damage. The study involved a sample of 90 patients who had undergone coronary angiography. The genotyping of Cys370Ser in exon 2 of the Klotho gene was performed using the polymerase chain reaction. The evaluation of oxidative DNA damage was conducted using the alkaline comet test. The findings of the study revealed that there was no statistically significant association between the distribution of alleles in the Klotho SNPs and the occurrence of coronary artery disorders. In the meantime, the total comet score frequency was significantly associated with the rs9527025 polymorphism. The results of our study indicate that Klotho gene variants, particularly the C370S polymorphism (rs9527025), might play a role in influencing oxidative DNA damage in age-related diseases, such as coronary artery disease. In consequence, larger studies are required to confirm the association between Klotho deficiency and the progression of cardiovascular disease in order to elucidate risk factors for coronary artery disease and develop potential therapeutic strategies.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Toxicology

Journal Section

Research Article

Authors

Seher Karslı ^*
0000-0002-4036-0399
Türkiye

Selma Yazar
0000-0003-2626-4066
Türkiye

Mehmet Karaca
0000-0001-8771-0539
Türkiye

Türkan Yurdun
0000-0002-2554-1204
Türkiye

Publication Date

June 28, 2025

Submission Date

August 7, 2023

Acceptance Date

August 23, 2023

Published in Issue

Year 2023 Volume: 27 Number: 5

IZ

https://izlik.org/JA73PS39RR

Cite

RIS / Bibtex

APA

Karslı, S., Yazar, S., Karaca, M., & Yurdun, T. (2025). Klotho gene polymorphism as a susceptibility factor for oxidative DNA damage in coronary artery disease. Journal of Research in Pharmacy, 27(5), 2079-2086. https://izlik.org/JA73PS39RR

AMA

1.Karslı S, Yazar S, Karaca M, Yurdun T. Klotho gene polymorphism as a susceptibility factor for oxidative DNA damage in coronary artery disease. J. Res. Pharm. 2025;27(5):2079-2086. https://izlik.org/JA73PS39RR

Chicago

Karslı, Seher, Selma Yazar, Mehmet Karaca, and Türkan Yurdun. 2025. “Klotho Gene Polymorphism As a Susceptibility Factor for Oxidative DNA Damage in Coronary Artery Disease”. Journal of Research in Pharmacy 27 (5): 2079-86. https://izlik.org/JA73PS39RR.

EndNote

Karslı S, Yazar S, Karaca M, Yurdun T (July 1, 2025) Klotho gene polymorphism as a susceptibility factor for oxidative DNA damage in coronary artery disease. Journal of Research in Pharmacy 27 5 2079–2086.

IEEE

[1]S. Karslı, S. Yazar, M. Karaca, and T. Yurdun, “Klotho gene polymorphism as a susceptibility factor for oxidative DNA damage in coronary artery disease”, J. Res. Pharm., vol. 27, no. 5, pp. 2079–2086, July 2025, [Online]. Available: https://izlik.org/JA73PS39RR

ISNAD

Karslı, Seher - Yazar, Selma - Karaca, Mehmet - Yurdun, Türkan. “Klotho Gene Polymorphism As a Susceptibility Factor for Oxidative DNA Damage in Coronary Artery Disease”. Journal of Research in Pharmacy 27/5 (July 1, 2025): 2079-2086. https://izlik.org/JA73PS39RR.

JAMA

1.Karslı S, Yazar S, Karaca M, Yurdun T. Klotho gene polymorphism as a susceptibility factor for oxidative DNA damage in coronary artery disease. J. Res. Pharm. 2025;27:2079–2086.

MLA

Karslı, Seher, et al. “Klotho Gene Polymorphism As a Susceptibility Factor for Oxidative DNA Damage in Coronary Artery Disease”. Journal of Research in Pharmacy, vol. 27, no. 5, July 2025, pp. 2079-86, https://izlik.org/JA73PS39RR.

Vancouver

1.Seher Karslı, Selma Yazar, Mehmet Karaca, Türkan Yurdun. Klotho gene polymorphism as a susceptibility factor for oxidative DNA damage in coronary artery disease. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(5):2079-86. Available from: https://izlik.org/JA73PS39RR