In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts

Ayşegül Çaşkurlu Öz; Şule Nur Karavuş; Sevde Nur Biltekin; Esra Zeynep Helvaci; Ayşe Esra Karadağ

In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts

Abstract

The aim of this study was to evaluate phytochemical composition of Ajuga reptans L. (Lamiaceae) aerial parts (separately flower and leaf) methanol, aqueous-methanolic extracts, and their cytotoxic activities. The phytochemical analysis was performed high-performance liquid chromatography (HPLC). Caffeic acid, p-coumaric, gallic, chlorogenic, ferulic acids, kaempferol, rutin, quercetin, quercetin-3-O-galactoside, and quercitrin were used as reference substances by HPLC in all samples. The major compounds in the extract were found as ferulic acid, caffeic acid, rutin, and quercetin-3-O-galactoside. Cytotoxicity was investigated using methyl thiazole tetrazolium (MTT) assay. Cytotoxic evaluation of the extracts against cancer (MCF7, PC3, and A549) and healthy human embryonic kidney cell line (HEK293) cell lines by MTT. Compared to other cells, the methanol extract of A. reptans demonstrated high selectivity against PC3 cells (IC50: 95  0.99 μg/mL) and selectivity index was four times higher than reference drug colchicine. (IC50: 95  0.99 μg/mL, SI: 6.10). A. reptans demonstrated antiproliferative potential against prostate and lung cancer cells. Therefore, additional investigations are needed to study the mechanism of the cytotoxicity for A. reptans.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Ayşegül Çaşkurlu Öz
0000-0001-7277-920X
Türkiye

Şule Nur Karavuş
0000-0001-9265-8042
Türkiye

Sevde Nur Biltekin
0000-0003-1896-2729
Türkiye

Esra Zeynep Helvaci This is me
0000-0003-4765-6026
Türkiye

Ayşe Esra Karadağ ^*
Türkiye

Publication Date

June 28, 2025

Submission Date

January 30, 2023

Acceptance Date

March 21, 2023

Published in Issue

Year 2023 Volume: 27 Number: 5

IZ

https://izlik.org/JA57UW89PM

Cite

RIS / Bibtex

APA

Çaşkurlu Öz, A., Karavuş, Ş. N., Biltekin, S. N., Helvaci, E. Z., & Karadağ, A. E. (2025). In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts. Journal of Research in Pharmacy, 27(5), 2115-2123. https://izlik.org/JA57UW89PM

AMA

1.Çaşkurlu Öz A, Karavuş ŞN, Biltekin SN, Helvaci EZ, Karadağ AE. In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts. J. Res. Pharm. 2025;27(5):2115-2123. https://izlik.org/JA57UW89PM

Chicago

Çaşkurlu Öz, Ayşegül, Şule Nur Karavuş, Sevde Nur Biltekin, Esra Zeynep Helvaci, and Ayşe Esra Karadağ. 2025. “In Vitro Cytotoxicity Evaluation and Phytochemical Analysis of Ajuga Reptans L. Extracts”. Journal of Research in Pharmacy 27 (5): 2115-23. https://izlik.org/JA57UW89PM.

EndNote

Çaşkurlu Öz A, Karavuş ŞN, Biltekin SN, Helvaci EZ, Karadağ AE (July 1, 2025) In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts. Journal of Research in Pharmacy 27 5 2115–2123.

IEEE

[1]A. Çaşkurlu Öz, Ş. N. Karavuş, S. N. Biltekin, E. Z. Helvaci, and A. E. Karadağ, “In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts”, J. Res. Pharm., vol. 27, no. 5, pp. 2115–2123, July 2025, [Online]. Available: https://izlik.org/JA57UW89PM

ISNAD

Çaşkurlu Öz, Ayşegül - Karavuş, Şule Nur - Biltekin, Sevde Nur - Helvaci, Esra Zeynep - Karadağ, Ayşe Esra. “In Vitro Cytotoxicity Evaluation and Phytochemical Analysis of Ajuga Reptans L. Extracts”. Journal of Research in Pharmacy 27/5 (July 1, 2025): 2115-2123. https://izlik.org/JA57UW89PM.

JAMA

1.Çaşkurlu Öz A, Karavuş ŞN, Biltekin SN, Helvaci EZ, Karadağ AE. In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts. J. Res. Pharm. 2025;27:2115–2123.

MLA

Çaşkurlu Öz, Ayşegül, et al. “In Vitro Cytotoxicity Evaluation and Phytochemical Analysis of Ajuga Reptans L. Extracts”. Journal of Research in Pharmacy, vol. 27, no. 5, July 2025, pp. 2115-23, https://izlik.org/JA57UW89PM.

Vancouver

1.Ayşegül Çaşkurlu Öz, Şule Nur Karavuş, Sevde Nur Biltekin, Esra Zeynep Helvaci, Ayşe Esra Karadağ. In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(5):2115-23. Available from: https://izlik.org/JA57UW89PM