Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements

Ümit Can Erim; Nese Ayşit

Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements

Abstract

Quercetin is a ubiquitous bioactive flavonoid found in broad selection of dietary supplements. It has potential beneficial health effects and induces therapeutic activity in many diseases including different types of cancer. Quercetin exerts cytotoxic activity in cancer and induces apoptotic cell death. Albeit, each dietary supplement consists of impurities, which in turn diminish the biological activity of the quercetin. Herein, we extracted quercetin from 5 different marketed dietary supplements in tablet formulation and detected their quercetin content by developing and validating an RP-HPLC method. We further investigated the cytotoxic activity of quercetin and its role in apoptosis through mitochondrial pathway in MCF-7 and 4T1 breast cancer cell lines. Our findings demonstrated that the quercetin content in all formulations was less than the amount stated in the labeling information, nevertheless they exerted significant cytotoxic activities in breast cancer cells. We revealed that formulations with high quercetin content induced apoptosis through restoring the tumor suppression activity of p53 and activating downstream caspases, whereas the formulation with low quercetin content potentially induced non-apoptotic cell death. Our work illustrates the involvement of quercetin in apop

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry

Journal Section

Research Article

Authors

Ümit Can Erim ^*
0000-0002-4121-588X
Türkiye

Nese Ayşit
0000-0002-4243-9092
Türkiye

Publication Date

June 28, 2025

Submission Date

October 25, 2023

Acceptance Date

February 28, 2024

Published in Issue

Year 2024 Volume: 28 Number: 5

IZ

https://izlik.org/JA59KB96PJ

Cite

RIS / Bibtex

APA

Erim, Ü. C., & Ayşit, N. (2025). Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. Journal of Research in Pharmacy, 28(5), 1653-1664. https://izlik.org/JA59KB96PJ

AMA

1.Erim ÜC, Ayşit N. Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. J. Res. Pharm. 2025;28(5):1653-1664. https://izlik.org/JA59KB96PJ

Chicago

Erim, Ümit Can, and Nese Ayşit. 2025. “Development and Validation of an RP-HPLC Method to Compare the Apoptotic Activity of Quercetin Found in Marketed Dietary Supplements”. Journal of Research in Pharmacy 28 (5): 1653-64. https://izlik.org/JA59KB96PJ.

EndNote

Erim ÜC, Ayşit N (July 1, 2025) Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. Journal of Research in Pharmacy 28 5 1653–1664.

IEEE

[1]Ü. C. Erim and N. Ayşit, “Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements”, J. Res. Pharm., vol. 28, no. 5, pp. 1653–1664, July 2025, [Online]. Available: https://izlik.org/JA59KB96PJ

ISNAD

Erim, Ümit Can - Ayşit, Nese. “Development and Validation of an RP-HPLC Method to Compare the Apoptotic Activity of Quercetin Found in Marketed Dietary Supplements”. Journal of Research in Pharmacy 28/5 (July 1, 2025): 1653-1664. https://izlik.org/JA59KB96PJ.

JAMA

1.Erim ÜC, Ayşit N. Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. J. Res. Pharm. 2025;28:1653–1664.

MLA

Erim, Ümit Can, and Nese Ayşit. “Development and Validation of an RP-HPLC Method to Compare the Apoptotic Activity of Quercetin Found in Marketed Dietary Supplements”. Journal of Research in Pharmacy, vol. 28, no. 5, July 2025, pp. 1653-64, https://izlik.org/JA59KB96PJ.

Vancouver

1.Ümit Can Erim, Nese Ayşit. Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. J. Res. Pharm. [Internet]. 2025 Jul. 1;28(5):1653-64. Available from: https://izlik.org/JA59KB96PJ