Effects of Surfactants on Albumin Nanoparticles

Yagmur Akdag; Zeynep Merve Geyik

Effects of Surfactants on Albumin Nanoparticles

Abstract

This study aimed to examine the effects of various surfactants on the particle size distribution of albumin (HSA) nanoparticles and the binding efficiency of the active substance to albumin. Therefore, gefitinib, an EGFR tyrosine kinase inhibitor and albumin nanoparticles were manufactured using Nab™ technology with various surfactants at different levels. Before producing gefitinib-albumin nanoparticles, the fluorescence spectroscopy method in the absence and presence of surfactants was used to demonstrate the binding of the gefitinib to albumin. Gefitinib binding to HSA in the presence and absence of surfactants, was demonstrated with Stern-Volmer plots. In order to optimize the nanoparticle production method, the effects of critical process parameters such as organic phase volume: total volume %, drug:HSA ratio, homogenization cycle number on particle size distribution were evaluated using the Box-Behnken design. After optimization of production method, the nanoparticles were produced by adding three different levels of DPPC, HSPC, and oleic acid to the formulation, and the effects of surfactants on the particle size distribution and zeta potential were evaluated. Adding surfactants had no statistically significant effect on the Stern Volmer plots but they helped to produce uniform nanoparticles with PDI and particle size values of less than 0.2 and 130 nm respectively. It was observed that adding HSPC, DPPC, or oleic acid to the formulation enabled the production of uniform albumin nanoparticles.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biotechnology

Journal Section

Research Article

Authors

Yagmur Akdag ^*
0000-0003-4139-9815
Türkiye

Zeynep Merve Geyik This is me
0000-0003-2775-6724
Türkiye

Publication Date

June 28, 2025

Submission Date

May 13, 2022

Acceptance Date

June 18, 2022

Published in Issue

Year 2022 Volume: 26 Number: 5

IZ

https://izlik.org/JA35TJ68HB

Cite

RIS / Bibtex

APA

Akdag, Y., & Geyik, Z. M. (2025). Effects of Surfactants on Albumin Nanoparticles. Journal of Research in Pharmacy, 26(5), 1177-1189. https://izlik.org/JA35TJ68HB

AMA

1.Akdag Y, Geyik ZM. Effects of Surfactants on Albumin Nanoparticles. J. Res. Pharm. 2025;26(5):1177-1189. https://izlik.org/JA35TJ68HB

Chicago

Akdag, Yagmur, and Zeynep Merve Geyik. 2025. “Effects of Surfactants on Albumin Nanoparticles”. Journal of Research in Pharmacy 26 (5): 1177-89. https://izlik.org/JA35TJ68HB.

EndNote

Akdag Y, Geyik ZM (June 1, 2025) Effects of Surfactants on Albumin Nanoparticles. Journal of Research in Pharmacy 26 5 1177–1189.

IEEE

[1]Y. Akdag and Z. M. Geyik, “Effects of Surfactants on Albumin Nanoparticles”, J. Res. Pharm., vol. 26, no. 5, pp. 1177–1189, June 2025, [Online]. Available: https://izlik.org/JA35TJ68HB

ISNAD

Akdag, Yagmur - Geyik, Zeynep Merve. “Effects of Surfactants on Albumin Nanoparticles”. Journal of Research in Pharmacy 26/5 (June 1, 2025): 1177-1189. https://izlik.org/JA35TJ68HB.

JAMA

1.Akdag Y, Geyik ZM. Effects of Surfactants on Albumin Nanoparticles. J. Res. Pharm. 2025;26:1177–1189.

MLA

Akdag, Yagmur, and Zeynep Merve Geyik. “Effects of Surfactants on Albumin Nanoparticles”. Journal of Research in Pharmacy, vol. 26, no. 5, June 2025, pp. 1177-89, https://izlik.org/JA35TJ68HB.

Vancouver

1.Yagmur Akdag, Zeynep Merve Geyik. Effects of Surfactants on Albumin Nanoparticles. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(5):1177-89. Available from: https://izlik.org/JA35TJ68HB