In vitro antidiabetic effect, quantitative studies and UPLC TOF-MS analysis of black tea samples from Turkish market

Nilüfer Orhan; Hasya Nazlı Gök; Murat Kadir Şüküroğlu; Mustafa Aslan

In vitro antidiabetic effect, quantitative studies and UPLC TOF-MS analysis of black tea samples from Turkish market

Abstract

In vitro antidiabetic effects of seven Turkish and five Sri Lankan black tea (Camelia sinensis) samples collected from Turkish market were investigated through enzyme inhibition assays. Foreign matter, non-oxidized particles, water soluble matter, loss on drying and total ash values were determined and compared with the Turkish Food Codex-Tea Notification and found to be compliant with the standards. Total phenolic and flavonoid contents, caffeine, gallic acid and rutin amounts of tea samples were measured. Ultra-pressure liquid chromatography with time of flight mass spectroscopy (UPLC-TOF-MS) was used to compare the major compounds of tea samples. All tea samples had potent inhibition against alpha glucosidase (>100-97.86% at 3 mg/ml). Water soluble matters of Sri Lankan teas are higher than them of Turkish samples (except TL1). Caffeine amount of the Sri Lankan tea samples were approximately twice of Turkish black tea samples. Sixteen common compounds were identified in tea samples by UPLC-TOF-MS.

Keywords

References

[1] Chang K. (Secretary). World Tea Production and Trade, Current and Future Development Food and Agriculture Organization of the United Nations, Rome, Italy 2015. http://www.fao.org/3/a-i4480e.pdf (accessed October 12, 2018).
[2] Kumar RSS, Murugesan S, Kottur G, Gyamfi D. Chapter 4: Black Tea: The plants, processing/manufacturing and production. Preedy VR (ed.) In: Tea in Health and Disease Prevention. Academic Press, London, United Kingdom 2013.
[3] Uchida N, Yoshimoto N, Nakamura A, Takagi A, Honda K, Moto M, Katsuraya K, Hashizume N. Difference of inhibitory effect of α-glucosidase by tea leaves species and extraction condition and effect of black tea on postprandial blood glucose level elevation in ICR mice. Int J Anal Bio-Sci. 2013; 36(2): 193-202.
[4] Lee M-S, Hwang Y-S, Lee J, Choung M-G. The characterization of caffeine and nine individual catechins in the leaves of green tea (Camellia sinensis L.) by near-infrared reflectance spectroscopy. Food Chem. 2014; 158: 351-357. [CrossRef]
[5] Tijburg LBM, Mattern T, Folts JD, Weisgerber UM, Katan M.B. Tea flavonoids and cardiovascular diseases: A review. Crit Rev Food Sci Nutr. 1997; 37(8): 771-785. [CrossRef]
[6] Dufresne CJ, Farnworth ER. A review of latest research findings on the health promotion properties of tea. J Nutr Biochem. 2001; 12: 404-421.
[7] Gardner EJ, Ruxton CHS, Leeds AR. Black tea - helpful or harmful? A review of the evidence. Eur J Clin Nutr. 2007; 61: 3-18. [CrossRef]
[8] Sharangi AB. Medicinal and therapeutic potentialities of tea (Camellia sinensis L.) - A review. Food Res Int. 2009; 42: 529-535. [CrossRef]

[9] Trevisanato SI, Kim Y-I. Tea and Health. Nutr Rev. 2000; 58(1): 1-10.
[10] Yang CS, Landau JM. Effects of tea consumption on nutrition and health. J Nutr. 2000; 130(10): 2409-2412. [CrossRef]
[11] Preedy VR. Tea in Health and Disease Prevention. Academic Press, London, United Kingdom 2013.
[12] Turkish Food Codex Tea Notification. Government Gazette of Turkish Republic 2015; Number: 29389, Notification number: 2015/30.
[13] Zuo Y, Chen H, Deng Y. Simultaneous determination of catechins, caffeine and gallic acids in green, oolong, black and pu-erh teas using HPLC with a photodiode array detector. Talanta. 2002; 57: 307-316.
[14] Sharma A, Bhondekar AP, Bari SS, Gulat, A, Kapur P, Singla ML. Development and optimization of an HPLC method for the routine analysis of catechins, caffeine, and gallic acid in tea (Camellia sinensis). J Liq Chromatogr Relat Technol. 2011; 34: 2243-2255. [CrossRef]
[15] Ediriweera ERHSS, Ratnasooriya WD. A review on herbs used in treatment of diabetes mellitus by Sri Lankan ayurvedic and traditional physicians. AYU. 2009; 30: 373-391.
[16] Gomes A, Vedasiromoni JR, Das M, Sharma RM, Ganguly DK. Anti-hyperglycemic effect of black tea (Camellia sinensis) in rat. J Ethnopharmacol. 1995; 45: 223-226.
[17] Jayakody JRAC, Ratnasooriya WD. Blood glucose level lowering activity of Sri Lankan Black tea brew (Camellia sinensis) in rats. Pharmacog Mag. 2008; 4(16): 341-349.
[18] Abeywickrama KRW, Ratnasooriy WD, Amarakoon AMT. Oral hypoglycaemic, antihyperglycaemic and antidiabetic activities of Sri Lankan Broken Orange Pekoe Fannings (BOPF) grade black tea (Camellia sinensis L.) in rats. J Ethnopharmacol. 2011; 135: 278-286. [CrossRef]
[19] Yilmazer-Musa M, Griffith AM, Michels AJ, Schneider E, Frei B. Grape seed and tea extracts and catechin 3-gallates are potent inhibitors of α-amylase and α-glucosidase activity. J Agric Food Chem. 2012; 60(36): 8924-8929. [CrossRef]
[20] Tang W, Li S, Liu Y, Huang M-T, Ho C-T. Anti-diabetic activity of chemically profiled green tea and black tea extracts in a type 2 diabetes mice model via different mechanisms. J Functional Foods. 2013; 5: 1784-1793.
[21] Jin D, Xu Y, Mei X, Meng Q, Gao Y, Li B, Tu Y. Antiobesity and lipid lowering effects of theaflavins on high-fat diet induced obese rats. J Functional Foods. 2013; 5: 1142-1150.
[22] Orhan N, Gökbulut A, Deliorman Orhan D. Antioxidant potential and carbohydrate digestive enzyme inhibitory effects of five Inula species and their major compounds. S Afr J Bot. 2017; 111: 86-92. [CrossRef]
[23] Orhan N, Aslan M, Şüküroğlu M, Deliorman Orhan D. In vivo and in vitro antidiabetic effect of Cistus laurifolius L. and detection of major phenolic compounds by UPLC–TOF-MS analysis. J Ethnopharmacol. 2013; 146(3): 859-865. [CrossRef]

Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Nilüfer Orhan ^*
Türkiye

Hasya Nazlı Gök
Türkiye

Murat Kadir Şüküroğlu
Türkiye

Mustafa Aslan
Türkiye

Publication Date

June 27, 2025

Submission Date

October 12, 2018

Acceptance Date

February 7, 2019

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA98KD67FH

Cite

RIS / Bibtex

APA

Orhan, N., Gök, H. N., Şüküroğlu, M. K., & Aslan, M. (2025). In vitro antidiabetic effect, quantitative studies and UPLC TOF-MS analysis of black tea samples from Turkish market. Journal of Research in Pharmacy, 23(3), 484-497. https://izlik.org/JA98KD67FH

AMA

1.Orhan N, Gök HN, Şüküroğlu MK, Aslan M. In vitro antidiabetic effect, quantitative studies and UPLC TOF-MS analysis of black tea samples from Turkish market. J. Res. Pharm. 2025;23(3):484-497. https://izlik.org/JA98KD67FH

Chicago

Orhan, Nilüfer, Hasya Nazlı Gök, Murat Kadir Şüküroğlu, and Mustafa Aslan. 2025. “In Vitro Antidiabetic Effect, Quantitative Studies and UPLC TOF-MS Analysis of Black Tea Samples from Turkish Market”. Journal of Research in Pharmacy 23 (3): 484-97. https://izlik.org/JA98KD67FH.

EndNote

Orhan N, Gök HN, Şüküroğlu MK, Aslan M (June 1, 2025) In vitro antidiabetic effect, quantitative studies and UPLC TOF-MS analysis of black tea samples from Turkish market. Journal of Research in Pharmacy 23 3 484–497.

IEEE

[1]N. Orhan, H. N. Gök, M. K. Şüküroğlu, and M. Aslan, “In vitro antidiabetic effect, quantitative studies and UPLC TOF-MS analysis of black tea samples from Turkish market”, J. Res. Pharm., vol. 23, no. 3, pp. 484–497, June 2025, [Online]. Available: https://izlik.org/JA98KD67FH

ISNAD

Orhan, Nilüfer - Gök, Hasya Nazlı - Şüküroğlu, Murat Kadir - Aslan, Mustafa. “In Vitro Antidiabetic Effect, Quantitative Studies and UPLC TOF-MS Analysis of Black Tea Samples from Turkish Market”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 484-497. https://izlik.org/JA98KD67FH.

JAMA

1.Orhan N, Gök HN, Şüküroğlu MK, Aslan M. In vitro antidiabetic effect, quantitative studies and UPLC TOF-MS analysis of black tea samples from Turkish market. J. Res. Pharm. 2025;23:484–497.

MLA

Orhan, Nilüfer, et al. “In Vitro Antidiabetic Effect, Quantitative Studies and UPLC TOF-MS Analysis of Black Tea Samples from Turkish Market”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 484-97, https://izlik.org/JA98KD67FH.

Vancouver

1.Nilüfer Orhan, Hasya Nazlı Gök, Murat Kadir Şüküroğlu, Mustafa Aslan. In vitro antidiabetic effect, quantitative studies and UPLC TOF-MS analysis of black tea samples from Turkish market. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):484-97. Available from: https://izlik.org/JA98KD67FH