Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils

İlker Demirbolat; Murat Kartal; Ünal Karık

Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils

Abstract

Black cumin (Nigella sativa L.) is a very popular medicinal plant around the world. Pharmacological effects have been widely studied over the years. It has an economic importance and being cultivated in many parts of Turkey and neighbour countries. Thymoquinone (TQ) is a volatile compound found in black cumin seeds and a major compound of its essential and fixed oils which has been reported as anticancer, antidiabetic, spasmolytic, immunomodulator, bronchodilator, antimicrobial, analgesic, anti-inflammatory, antioxidant etc. The quality of black cumin seed oil depends on extraction technics (expression, super critic CO2 extraction etc.), quality of seeds, storage time and thymoquinone content. In this study we developed and validated a rapid quantification GC-FID method to determine the thymoquinone levels in seed oils which uses diethyl phthalate (DEP) as an internal standard.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Chemistry

Journal Section

Research Article

Authors

İlker Demirbolat ^*
Türkiye

Murat Kartal
Türkiye

Ünal Karık
Türkiye

Publication Date

June 27, 2025

Submission Date

October 5, 2018

Acceptance Date

December 10, 2018

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA92XP22WJ

Cite

RIS / Bibtex

APA

Demirbolat, İ., Kartal, M., & Karık, Ü. (2025). Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. Journal of Research in Pharmacy, 23(3), 506-513. https://izlik.org/JA92XP22WJ

AMA

1.Demirbolat İ, Kartal M, Karık Ü. Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. J. Res. Pharm. 2025;23(3):506-513. https://izlik.org/JA92XP22WJ

Chicago

Demirbolat, İlker, Murat Kartal, and Ünal Karık. 2025. “Development and Validation of a GC-FID Method to Quantify Thymoquinone in Black Cumin Seed Oils”. Journal of Research in Pharmacy 23 (3): 506-13. https://izlik.org/JA92XP22WJ.

EndNote

Demirbolat İ, Kartal M, Karık Ü (June 1, 2025) Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. Journal of Research in Pharmacy 23 3 506–513.

IEEE

[1]İ. Demirbolat, M. Kartal, and Ü. Karık, “Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils”, J. Res. Pharm., vol. 23, no. 3, pp. 506–513, June 2025, [Online]. Available: https://izlik.org/JA92XP22WJ

ISNAD

Demirbolat, İlker - Kartal, Murat - Karık, Ünal. “Development and Validation of a GC-FID Method to Quantify Thymoquinone in Black Cumin Seed Oils”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 506-513. https://izlik.org/JA92XP22WJ.

JAMA

1.Demirbolat İ, Kartal M, Karık Ü. Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. J. Res. Pharm. 2025;23:506–513.

MLA

Demirbolat, İlker, et al. “Development and Validation of a GC-FID Method to Quantify Thymoquinone in Black Cumin Seed Oils”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 506-13, https://izlik.org/JA92XP22WJ.

Vancouver

1.İlker Demirbolat, Murat Kartal, Ünal Karık. Development and validation of a GC-FID method to quantify thymoquinone in black cumin seed oils. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):506-13. Available from: https://izlik.org/JA92XP22WJ