Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations

Ayşe Esra Karadağ; Betül Demirci; Zeynep Zişan Şakar; Sevde Nur Biltekin

Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations

Abstract

Salvia sclarea L. and Citrus aurantium L. are used ethnobotanically for wound treatment. The aim of this study was to evaluate phytochemical composition of Salvia sclarea L. and Citrus aurantium L. leaf essential oils and wound healing activities of their combinations. The phytochemical analysis was performed gas chromatography (GC) and mass spectroscopy (MS). Major components of S. sclarea leaf essential oil was confirmed both by GC-FID and GC/MS as 67% linalyl acetate, 20.2% linalool, 2.6%α-terpineole, respectively. The major components of C. aurantium leaf essential oil was also confirmed as 55% linalyl acetate, 23.4% linalool, 5.4%α-terpineole, respectively. Combinations of essential oils were loaded into Carbopol gel formulations. Wound healing activity of essential oil and combinations was investigated using in vitro scratch assay. As a result of the study, it was determined that the most effective wound healing concentration for Salvia sclarea essential oil was 50 µg/mL. The most effective concentration of Citrus aurantifolia essential oil was determined as 12.5 µg/mL. The aim of the study is to investigate the wound healing activities of the tested essential oils individually and in combination. The fact that no activity is observed in empty formulations is evidence that the activity of the loaded formulations does not come from empty formulations. It was observed that the loaded formulations showed better effects than the control Madecassol. Studies need to be confirmed by working under in vivo conditions.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Ayşe Esra Karadağ ^*
0000-0002-3412-0807
Türkiye

Betül Demirci
Türkiye

Zeynep Zişan Şakar This is me
0009-0000-8551-397X
Türkiye

Sevde Nur Biltekin
0000-0003-1896-2729
Türkiye

Publication Date

June 28, 2025

Submission Date

July 25, 2024

Acceptance Date

August 23, 2024

Published in Issue

Year 2024 Volume: 28 Number: 5

IZ

https://izlik.org/JA94MX66ED

Cite

RIS / Bibtex

APA

Karadağ, A. E., Demirci, B., Şakar, Z. Z., & Biltekin, S. N. (2025). Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations. Journal of Research in Pharmacy, 28(5), 1820-1828. https://izlik.org/JA94MX66ED

AMA

1.Karadağ AE, Demirci B, Şakar ZZ, Biltekin SN. Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations. J. Res. Pharm. 2025;28(5):1820-1828. https://izlik.org/JA94MX66ED

Chicago

Karadağ, Ayşe Esra, Betül Demirci, Zeynep Zişan Şakar, and Sevde Nur Biltekin. 2025. “Investigation of in Vitro Wound Healing Properties of Salvia Sclarea and Citrus Aurantium Essential Oil Combinations”. Journal of Research in Pharmacy 28 (5): 1820-28. https://izlik.org/JA94MX66ED.

EndNote

Karadağ AE, Demirci B, Şakar ZZ, Biltekin SN (July 1, 2025) Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations. Journal of Research in Pharmacy 28 5 1820–1828.

IEEE

[1]A. E. Karadağ, B. Demirci, Z. Z. Şakar, and S. N. Biltekin, “Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations”, J. Res. Pharm., vol. 28, no. 5, pp. 1820–1828, July 2025, [Online]. Available: https://izlik.org/JA94MX66ED

ISNAD

Karadağ, Ayşe Esra - Demirci, Betül - Şakar, Zeynep Zişan - Biltekin, Sevde Nur. “Investigation of in Vitro Wound Healing Properties of Salvia Sclarea and Citrus Aurantium Essential Oil Combinations”. Journal of Research in Pharmacy 28/5 (July 1, 2025): 1820-1828. https://izlik.org/JA94MX66ED.

JAMA

1.Karadağ AE, Demirci B, Şakar ZZ, Biltekin SN. Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations. J. Res. Pharm. 2025;28:1820–1828.

MLA

Karadağ, Ayşe Esra, et al. “Investigation of in Vitro Wound Healing Properties of Salvia Sclarea and Citrus Aurantium Essential Oil Combinations”. Journal of Research in Pharmacy, vol. 28, no. 5, July 2025, pp. 1820-8, https://izlik.org/JA94MX66ED.

Vancouver

1.Ayşe Esra Karadağ, Betül Demirci, Zeynep Zişan Şakar, Sevde Nur Biltekin. Investigation of in vitro wound healing properties of Salvia sclarea and Citrus aurantium essential oil combinations. J. Res. Pharm. [Internet]. 2025 Jul. 1;28(5):1820-8. Available from: https://izlik.org/JA94MX66ED