Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells

Erkan Civelek; Dilek Ozturk Civelek

Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells

Abstract

Nitric oxide (NO) is a highly reactive molecule involved in a variety of physiological and pathophysiological processes, such as inflammation. eNOS-produced NO has anti-inflammatory properties and play a role in vascular homeostasis. Through a variety of mechanisms, tumor necrosis factor alpha (TNF-α) has been shown to induce inflammation in HUVECs. The purpose of this study was to investigate the role of NO in HUVEC cells under inflammatory conditions induced by TNF-α. To identify TNF-α induction mechanisms, the phosphorylation of ERK, Akt, and eNOS was investigated. Sildenafil and L-NAME used to examine the role of NO in this process. In TNF-α-induced HUVEC cells, cell viability, nitrite/nitrate production, phosphorylated and total ERK, Akt, and eNOS levels were measured with or without sildenafil and L-NAME. At 20 and 40 ng/ml concentrations, TNF-α increased nitrite/nitrate and decreased cell viability (p<0.05). Sildenafil and L-NAME had no effect on cell viability and they both decreased nitrite/ nitrate in HUVEC that had been stimulated by TNF-α. TNF-α had no effect on the phosphorylation of ERK, Akt, and eNOS, but it did increase total eNOS levels. The phosphorylation of ERK, Akt, and eNOS was unaffected by sildenafil and L-NAME; however, L-NAME decreased total eNOS. According to our findings, there is no known direct relationship between TNF-α, sildenafil, or L-NAME and protein phosphorylation.

Keywords

References

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Details

Primary Language

English

Subjects

Medical Pharmacology

Journal Section

Research Article

Authors

Erkan Civelek
0000-0002-6503-5226
Türkiye

Dilek Ozturk Civelek ^* This is me
Türkiye

Publication Date

June 28, 2025

Submission Date

January 7, 2023

Acceptance Date

February 25, 2023

Published in Issue

Year 2023 Volume: 27 Number: 4

IZ

https://izlik.org/JA59GJ65CR

Cite

RIS / Bibtex

APA

Civelek, E., & Ozturk Civelek, D. (2025). Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. Journal of Research in Pharmacy, 27(4), 1560-1566. https://izlik.org/JA59GJ65CR

AMA

1.Civelek E, Ozturk Civelek D. Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. J. Res. Pharm. 2025;27(4):1560-1566. https://izlik.org/JA59GJ65CR

Chicago

Civelek, Erkan, and Dilek Ozturk Civelek. 2025. “Investigation the Role of Nitric Oxide Pathway in TNF-α Induced HUVEC Cells”. Journal of Research in Pharmacy 27 (4): 1560-66. https://izlik.org/JA59GJ65CR.

EndNote

Civelek E, Ozturk Civelek D (July 1, 2025) Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. Journal of Research in Pharmacy 27 4 1560–1566.

IEEE

[1]E. Civelek and D. Ozturk Civelek, “Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells”, J. Res. Pharm., vol. 27, no. 4, pp. 1560–1566, July 2025, [Online]. Available: https://izlik.org/JA59GJ65CR

ISNAD

Civelek, Erkan - Ozturk Civelek, Dilek. “Investigation the Role of Nitric Oxide Pathway in TNF-α Induced HUVEC Cells”. Journal of Research in Pharmacy 27/4 (July 1, 2025): 1560-1566. https://izlik.org/JA59GJ65CR.

JAMA

1.Civelek E, Ozturk Civelek D. Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. J. Res. Pharm. 2025;27:1560–1566.

MLA

Civelek, Erkan, and Dilek Ozturk Civelek. “Investigation the Role of Nitric Oxide Pathway in TNF-α Induced HUVEC Cells”. Journal of Research in Pharmacy, vol. 27, no. 4, July 2025, pp. 1560-6, https://izlik.org/JA59GJ65CR.

Vancouver

1.Erkan Civelek, Dilek Ozturk Civelek. Investigation the role of nitric oxide pathway in TNF-α induced HUVEC cells. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(4):1560-6. Available from: https://izlik.org/JA59GJ65CR