Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium

Dhanaraju Kavitha; Ramakrishnan Padmini; Thiyagarajan Deepan; Magharla Dasaratha Dhanaraju

Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium

Abstract

Green nanoparticle synthesis using biological systems, particularly plant extracts is a growing subject in nanotechnology. Leaves extract of Syringodium isoetifolium was used to successfully show the environmentally friendly manufacture of zinc oxide nanoparticles (ZnO NPs) and zinc nitrate were used as precursor in this study. The nanoparticles were examined using X-Ray Diffraction (XRD), Energy-Dispersive X-ray analysis (EDX), UV spectroscopy (UV-VIS), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FT-IR). The absorption peak in 223 nm range was discovered via UV–Vis spectroscopy. XRD and FTIR verified the involvement of Syringodium isoetifolium bioactive substances in the steadiness of zinc oxide nanoparticles. SEM and EDX investigation indicated an agglomerated asymmetrical, hexagonal morphology and presence of O, Zn, C and K. Elemental mapping study of produced ZnO NPs revealed 60% zinc distribution, 26% oxygen distribution, and 13% carbon distribution. The produced ZnO NPs had a mean particle size of 26 nm was determined using dynamic light scattering (DLS) measurements. Additionally, an in vitro assay was used to assess the ZnO NPs' cytotoxic effects on the HepG2 cell line. The ZnO NPs had a strong cytotoxic effect on the HepG2 cancer cell line, as shown by the results of the MTT assay. ZnO NPs exhibited excellent antimicrobial potencies against four different bacterial via pour plate method, and their zone of inhibition values were calculated.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry

Journal Section

Research Article

Authors

Dhanaraju Kavitha
0000-0003-3745-7939
India

Ramakrishnan Padmini ^*
0000-0002-0816-3600
India

Thiyagarajan Deepan This is me
0000-0003-4062-3993
India

Magharla Dasaratha Dhanaraju
0000-0003-4876-7051
India

Publication Date

June 27, 2025

Submission Date

August 11, 2022

Acceptance Date

October 23, 2022

Published in Issue

Year 2023 Volume: 27 Number: 2

IZ

https://izlik.org/JA73ZU56MZ

Cite

RIS / Bibtex

APA

Kavitha, D., Padmini, R., Deepan, T., & Dhanaraju, M. D. (2025). Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium. Journal of Research in Pharmacy, 27(2), 783-793. https://izlik.org/JA73ZU56MZ

AMA

1.Kavitha D, Padmini R, Deepan T, Dhanaraju MD. Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium. J. Res. Pharm. 2025;27(2):783-793. https://izlik.org/JA73ZU56MZ

Chicago

Kavitha, Dhanaraju, Ramakrishnan Padmini, Thiyagarajan Deepan, and Magharla Dasaratha Dhanaraju. 2025. “Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium Isoetifolium”. Journal of Research in Pharmacy 27 (2): 783-93. https://izlik.org/JA73ZU56MZ.

EndNote

Kavitha D, Padmini R, Deepan T, Dhanaraju MD (June 1, 2025) Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium. Journal of Research in Pharmacy 27 2 783–793.

IEEE

[1]D. Kavitha, R. Padmini, T. Deepan, and M. D. Dhanaraju, “Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium”, J. Res. Pharm., vol. 27, no. 2, pp. 783–793, June 2025, [Online]. Available: https://izlik.org/JA73ZU56MZ

ISNAD

Kavitha, Dhanaraju - Padmini, Ramakrishnan - Deepan, Thiyagarajan - Dhanaraju, Magharla Dasaratha. “Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium Isoetifolium”. Journal of Research in Pharmacy 27/2 (June 1, 2025): 783-793. https://izlik.org/JA73ZU56MZ.

JAMA

1.Kavitha D, Padmini R, Deepan T, Dhanaraju MD. Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium. J. Res. Pharm. 2025;27:783–793.

MLA

Kavitha, Dhanaraju, et al. “Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium Isoetifolium”. Journal of Research in Pharmacy, vol. 27, no. 2, June 2025, pp. 783-9, https://izlik.org/JA73ZU56MZ.

Vancouver

1.Dhanaraju Kavitha, Ramakrishnan Padmini, Thiyagarajan Deepan, Magharla Dasaratha Dhanaraju. Green Synthesis of Zinc Oxide Nanoparticles, Characterization, Antibacterial and Cytotoxicity Against HepG2 Cells Using Syringodium isoetifolium. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(2):783-9. Available from: https://izlik.org/JA73ZU56MZ