The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study

Cebrail Akyüz; Emine Erdağ; Ayliz Velioglu-ogunc; Nurettin Abacioglu; Ahmet Ozer Sehirli

The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study

Abstract

In this study, we aimed to determine the protective effect of grape seed extract against mesenteric ischemia/reperfusion (I/R) injury in rats. In addition, an in silico molecular docking study was performed to compare the effects of natural compounds in grape seed extract on myeloperoxidase (MPO) enzyme at the molecular level. In addition to MPO activity, malondialdehyde (MDA) and glutathione (GSH) activities were also measured in the intestinal tissue samples. Serum cytokines, TNF-α and IL-1β, were measured to assess systemic tissue damage. Mesenteric GSH decreased significantly after ischemia/reperfusion, while MDA levels and MPO activity increased significantly. Binding interactions were detected by the binding mode of analysis, and the study compared the binding affinities of compounds at the active site of myeloperoxidase and glutathione peroxidase. As a result, it was found that procyanidins (B1-B4), which are predominantly present in GS extract, have the highest binding affinity and molecular interaction.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Cebrail Akyüz
0000-0003-0917-9345
Türkiye

Emine Erdağ ^*
Türkiye

Ayliz Velioglu-ogunc This is me
0000-0001-8453-6491
Türkiye

Nurettin Abacioglu This is me
0000-0001-6609-1505
Türkiye

Ahmet Ozer Sehirli This is me
Türkiye

Publication Date

June 28, 2025

Submission Date

January 18, 2023

Acceptance Date

March 16, 2023

Published in Issue

Year 2023 Volume: 27 Number: 4

IZ

https://izlik.org/JA86YS22TG

Cite

RIS / Bibtex

APA

Akyüz, C., Erdağ, E., Velioglu-ogunc, A., Abacioglu, N., & Sehirli, A. O. (2025). The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. Journal of Research in Pharmacy, 27(4), 1513-1523. https://izlik.org/JA86YS22TG

AMA

1.Akyüz C, Erdağ E, Velioglu-ogunc A, Abacioglu N, Sehirli AO. The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. J. Res. Pharm. 2025;27(4):1513-1523. https://izlik.org/JA86YS22TG

Chicago

Akyüz, Cebrail, Emine Erdağ, Ayliz Velioglu-ogunc, Nurettin Abacioglu, and Ahmet Ozer Sehirli. 2025. “The Protective Effect of Grape Seed Extract (Vitis Vinifera) Against Mesenteric Ischemia Reperfusion Injury in Rats: An in Vivo, in Vitro and Molecular Docking Study”. Journal of Research in Pharmacy 27 (4): 1513-23. https://izlik.org/JA86YS22TG.

EndNote

Akyüz C, Erdağ E, Velioglu-ogunc A, Abacioglu N, Sehirli AO (July 1, 2025) The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. Journal of Research in Pharmacy 27 4 1513–1523.

IEEE

[1]C. Akyüz, E. Erdağ, A. Velioglu-ogunc, N. Abacioglu, and A. O. Sehirli, “The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study”, J. Res. Pharm., vol. 27, no. 4, pp. 1513–1523, July 2025, [Online]. Available: https://izlik.org/JA86YS22TG

ISNAD

Akyüz, Cebrail - Erdağ, Emine - Velioglu-ogunc, Ayliz - Abacioglu, Nurettin - Sehirli, Ahmet Ozer. “The Protective Effect of Grape Seed Extract (Vitis Vinifera) Against Mesenteric Ischemia Reperfusion Injury in Rats: An in Vivo, in Vitro and Molecular Docking Study”. Journal of Research in Pharmacy 27/4 (July 1, 2025): 1513-1523. https://izlik.org/JA86YS22TG.

JAMA

1.Akyüz C, Erdağ E, Velioglu-ogunc A, Abacioglu N, Sehirli AO. The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. J. Res. Pharm. 2025;27:1513–1523.

MLA

Akyüz, Cebrail, et al. “The Protective Effect of Grape Seed Extract (Vitis Vinifera) Against Mesenteric Ischemia Reperfusion Injury in Rats: An in Vivo, in Vitro and Molecular Docking Study”. Journal of Research in Pharmacy, vol. 27, no. 4, July 2025, pp. 1513-2, https://izlik.org/JA86YS22TG.

Vancouver

1.Cebrail Akyüz, Emine Erdağ, Ayliz Velioglu-ogunc, Nurettin Abacioglu, Ahmet Ozer Sehirli. The protective effect of grape seed extract (Vitis vinifera) against mesenteric ischemia/reperfusion injury in rats: an in vivo, in vitro and molecular docking study. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(4):1513-2. Available from: https://izlik.org/JA86YS22TG