Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode

Year 2022, Volume: 26 Issue: 5, 1484 - 1492, 28.06.2025

Bilal Tunç Karina Amudi Pınar Talay Pınar

Abstract

In this study, electrochemical analysis of cefotaxime, one of the third-generation cephalosporins, the semisynthetic
antibiotic group of the -Lactam family, with an anodic pre-treated boron-doped diamond electrode (APTBDDE)
were investigated in a wide pH range. The cyclic voltametric (CV) and square-wave voltametric (SWV)
techniques were applied for the rapid, sensitive, cheap, simple, and precise detection of CEF. The compound yielded
two well-defined oxidation steps (Ia=1.1; IIa=1.5 V) in acidic, neutral, and alkaline media by employing SWV.
Experimental conditions, namely pH, modulation amplitude, step potential, frequency, interference, and effect of scan
rate were optimized. By employing SWV, there was a linear dependence of the oxidation peak current at +1.1 V and
CEF concentration in the range of 1.0-20.0 μg mL-1 (2.1×10-6 – 4.2×10-5 mol L-1), with a detection limit of 0.22 μg mL-1
(4.6×10-7 mol L-1) in 0.1 mol L-1 phosphate buffer solution (pH 2.5). The practical applicability of the proposed method
was tested with the quantification of CEF in the drug formulation.

Keywords

Cephalosporins , cefotaxime analysis , boron-doped diamond electrode , voltammetry , determination in drug formulation

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