Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode

Bilal Tunç; Karina Amudi; Pınar Talay Pınar

Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode

Abstract

In this study, electrochemical analysis of cefotaxime, one of the third-generation cephalosporins, the semisynthetic antibiotic group of the -Lactam family, with an anodic pre-treated boron-doped diamond electrode (APTBDDE) were investigated in a wide pH range. The cyclic voltametric (CV) and square-wave voltametric (SWV) techniques were applied for the rapid, sensitive, cheap, simple, and precise detection of CEF. The compound yielded two well-defined oxidation steps (Ia=1.1; IIa=1.5 V) in acidic, neutral, and alkaline media by employing SWV. Experimental conditions, namely pH, modulation amplitude, step potential, frequency, interference, and effect of scan rate were optimized. By employing SWV, there was a linear dependence of the oxidation peak current at +1.1 V and CEF concentration in the range of 1.0-20.0 μg mL-1 (2.1×10-6 – 4.2×10-5 mol L-1), with a detection limit of 0.22 μg mL-1 (4.6×10-7 mol L-1) in 0.1 mol L-1 phosphate buffer solution (pH 2.5). The practical applicability of the proposed method was tested with the quantification of CEF in the drug formulation.

Keywords

References

[1] Nemutlu E, Kır S. Üçüncü ve Dördüncü Kuşaktan Seçilmiş Sefalosporinlerin İnsan Kaynaklı Biyolojik Materyallerden HPLC ile Tayinleri. Hacet Univ J Fac Pharm. 2009; 28(2): 185-207.
[2] Yıldız İ, Varkal MA, Ünüvar E. Günümüzde Sefalosporinler ve Antibiyotik Direnci. Çocuk Dergisi, 2014; 14(1), 22- 27.
[3] Bellouard R, Deslandes G, Morival C, Li J, Boutoille D, Jolliet P, Grégoire M. Simultaneous Determination of Eight Β- Lactam Antibiotics in Human Plasma and Cerebrospinal Fluid by Liquid Chromatography Coupled to Tandem Mass Spectrometry. J Pharm Biomed Anal. 2020;178, 112904. [CrossRef]
[4] Ecke A, Schneider RJ. Pitfalls in the Immunochemical Determination of β-Lactam Antibiotics in Water. Antibiotics 2021;10(3), 298. [CrossRef]
[5] Dou M, Wang J, Gao B, Xu C, Yang F. Photocatalytic Difference of Amoxicillin and Cefotaxime Under Visible Light by Mesoporous G-C3N4: Mechanism, Degradation Pathway and DFT Calculation. Chem Eng J. 2020;383, 123134. [CrossRef]
[6] Arabsorkhi B, Sereshti H. Determination of Tetracycline and Cefotaxime Residues in Honey by Micro-solid Phase Extraction Based on Electrospun Nanofibers Coupled with HPLC. Microchem J. 2018;140, 241-247. [CrossRef]
[7] Baluchová S, Daňhel A, Dejmková H, Ostatná V, Fojta M, Schwarzová-Pecková K. Recent Progress in the Applications of Boron-doped Diamond Electrodes in Electroanalysis of Organic Compounds and Biomolecules–A Review. Anal chim acta 2019;1077, 30-66. [CrossRef]
[8] He Y, Lin H, Guo Z, Zhang W, Li H, Huang W. Recent developments and advances in boron-doped diamond electrodes for electrochemical oxidation of organic pollutants. Sep Purif Technol. 2019; 212, 802-821. [CrossRef]

[9] Clematis D, Panizza M. Application of Boron-doped Diamond Electrodes for Electrochemical Oxidation of Real Wastewaters. Curr Opin Electrochem. 2021;30, 100844. [CrossRef]
[10] Talay Pınar P, Allahverdiyeva S, Yardım Y, Şentürk Z. Voltammetric Sensing of Dinitrophenolic Herbicide Dinoterb on Cathodically Pretreated Boron-doped Diamond Electrode in the Presence of Cationic Surfactant. Microchem J. 2020;155, 104772. [CrossRef]
[11] Allahverdiyeva S, Talay Pınar P, Yardım Y, Şentürk Z. The First Report for the Electrochemical Investigation of a New HIV Integrase Inhibitor Dolutegravir: Its Voltammetric Determination in Tablet Dosage Forms and Human Urine Using a Boron-Doped Diamond Electrode. Diam Relat Mater. 2021;114, 108332. [CrossRef]
[12] Lourencao BC, Brocenschi RF, Medeiros RA, Fatibello‐Filho O, Rocha‐Filho RC. Analytical Applications of Electrochemically Pretreated Boron‐doped Diamond Electrodes. Chem Electro Chem. 2020;7(6),1291-1311. [CrossRef]
[13] Sousa CP, Ribeiro FW, Oliveira TM, Salazar‐Banda GR, de Lima‐Neto P, Morais S, Correia AN. Electroanalysis of Pharmaceuticals on Boron‐doped Diamond Electrodes: a Review. Chem Electro Chem. 2019;6(9), 2350-2378. [CrossRef]
[14] Talay Pınar P, Senturk Z. Voltammetric Investigation of Antiviral Drug Valacyclovir at a Boron-doped Diamond Electrode in Different Electrolyte Media: Its Determination Enhanced by Anionic Surfactant in Pharmaceuticals and Biological Fluids. Curr Pharma Anal. 2017;13(2), 175-187.
[15] Talay Pınar P, Yardım Y, Şentürk Z. Individual and Simultaneous Electroanalytical Sensing of Epinephrine and Lidocaine Using an Anodically Pretreated Boron-doped Diamond Electrode by Square-wave Voltammetry. Diam Relat Mater. 2020;101, 107649. [CrossRef]
[16] Aleksić MM, Kapetanović V. Voltammetric Behavior and Square-wave Voltammetric Determination of Cefotaxime in Urine. J Electroanal Chem. 2006;593(1-2), 258-266. [CrossRef]
[17] Dogan B, Golcu A, Dolaz M, Ozkan SA. Anodic Oxidation of Antibacterial Drug Cefotaxime Sodium and Its Square wave and Differential Pulse Voltammetric Determination in Pharmaceuticals and Human Serum. Curr Pharm Anal. 2009;5(2), 197-207. [CrossRef]
[18] Zhang F, Gu S, Ding Y, Zhou L, Zhang Z, Li L. Electrooxidation and Determination of Cefotaxime on Au Nanoparticles/poly (L-arginine) Modified Carbon Paste Electrode. J Electroanal Chem. 2013;698, 25-30. [CrossRef]
[19] Vasantharaghavan V, Cingaram R. Voltammetry Determination of Cefotaxime on Zinc Oxide Nanorod Modified Electrode. Curr Pharma Anal. 2021;17(1), 40-46. [CrossRef]
[20] Patil MM, Shetti NP, Malode SJ, Nayak DS, Chakklabbi TR. Electroanalysis of Paracetamol at Nanoclay Modified Graphite Electrode. Materi Today: Procee. 2019;18, 986-993. [CrossRef]
[21] Kanyong P, Rawlinson S, Davis J. Fabrication and Electrochemical Characterization of Polydopamine Redox Polymer Modified Screen-printed Carbon Electrode for the Detection of Guanine. Sens Actuators B: Chem. 2016;233, 528-534. [CrossRef]
[22] Feier B, Gui A, Cristea C, Săndulescu R. Electrochemical Determination of Cephalosporins Using a Bare Boron-doped Diamond Electrode. Anal chim acta 2017;976, 25-34. [CrossRef]

Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry

Journal Section

Research Article

Authors

Bilal Tunç This is me
0000-0001-6722-0364
Türkiye

Karina Amudi This is me
0000-0002-2022-5501
Türkiye

Pınar Talay Pınar ^*
0000-0003-1027-1456
Türkiye

Publication Date

June 28, 2025

Submission Date

March 7, 2022

Acceptance Date

May 27, 2022

Published in Issue

Year 2022 Volume: 26 Number: 5

IZ

https://izlik.org/JA37HC85UR

Cite

RIS / Bibtex

APA

Tunç, B., Amudi, K., & Talay Pınar, P. (2025). Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode. Journal of Research in Pharmacy, 26(5), 1484-1492. https://izlik.org/JA37HC85UR

AMA

1.Tunç B, Amudi K, Talay Pınar P. Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode. J. Res. Pharm. 2025;26(5):1484-1492. https://izlik.org/JA37HC85UR

Chicago

Tunç, Bilal, Karina Amudi, and Pınar Talay Pınar. 2025. “Electrochemical Determination of Cefotaxime, a Cephalosporin Group Drug, at a Boron-Doped Diamond Electrode”. Journal of Research in Pharmacy 26 (5): 1484-92. https://izlik.org/JA37HC85UR.

EndNote

Tunç B, Amudi K, Talay Pınar P (June 1, 2025) Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode. Journal of Research in Pharmacy 26 5 1484–1492.

IEEE

[1]B. Tunç, K. Amudi, and P. Talay Pınar, “Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode”, J. Res. Pharm., vol. 26, no. 5, pp. 1484–1492, June 2025, [Online]. Available: https://izlik.org/JA37HC85UR

ISNAD

Tunç, Bilal - Amudi, Karina - Talay Pınar, Pınar. “Electrochemical Determination of Cefotaxime, a Cephalosporin Group Drug, at a Boron-Doped Diamond Electrode”. Journal of Research in Pharmacy 26/5 (June 1, 2025): 1484-1492. https://izlik.org/JA37HC85UR.

JAMA

1.Tunç B, Amudi K, Talay Pınar P. Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode. J. Res. Pharm. 2025;26:1484–1492.

MLA

Tunç, Bilal, et al. “Electrochemical Determination of Cefotaxime, a Cephalosporin Group Drug, at a Boron-Doped Diamond Electrode”. Journal of Research in Pharmacy, vol. 26, no. 5, June 2025, pp. 1484-92, https://izlik.org/JA37HC85UR.

Vancouver

1.Bilal Tunç, Karina Amudi, Pınar Talay Pınar. Electrochemical determination of cefotaxime, a cephalosporin group drug, at a boron-doped diamond electrode. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(5):1484-92. Available from: https://izlik.org/JA37HC85UR