Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species

Selman Şabanoğlu; Alper Gökbulut; Mehmet Levent Altun

Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species

Abstract

Medicinal plants used in traditional medicine have been increasingly noticed within recent years in pharmaceutical, cosmetic and nutraceutical industry. Herein, methanolic extracts of Achillea biebersteinii Afan., A. setacea Waldst. Et Kit. and A. wilhelmsii C. Koch were investigated for their total phenolic content and antioxidant activity with the main focus on phenolics. The total phenolic content varied widely in different parts of the three tested Achillea species, ranging from 113.2±1.9 to 178.4±9.5 mg GAE/g extract using Folin Ciocalteu method. DPPH and ABTS antioxidant activity tests were used to evaluate and compare the antioxidant activity of the species. Both the DPPH and ABTS assay results revealed that the radical scavenging activity of A. biebersteinii leaf extract is remarkably high with IC50 values of 0.377±0.011 and 0.016±0.002 mg/mL, respectively. Furthermore, a newly validated RP-HPLC-DAD method was developed and used to determine the phenolic compound profile of the methanol extracts. Phenolic components, such as gallic acid, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, rutin, quercetin, luteolin, apigenin and kaempferol were analyzed by HPLC-DAD. The significant antioxidant properties of the extracts could be attributed to the phenolics of the Achillea species.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Selman Şabanoğlu This is me
Türkiye

Alper Gökbulut ^*
Türkiye

Mehmet Levent Altun
Türkiye

Publication Date

June 27, 2025

Submission Date

September 21, 2018

Acceptance Date

January 26, 2019

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA98YT45KE

Cite

RIS / Bibtex

APA

Şabanoğlu, S., Gökbulut, A., & Altun, M. L. (2025). Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. Journal of Research in Pharmacy, 23(3), 567-576. https://izlik.org/JA98YT45KE

AMA

1.Şabanoğlu S, Gökbulut A, Altun ML. Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. J. Res. Pharm. 2025;23(3):567-576. https://izlik.org/JA98YT45KE

Chicago

Şabanoğlu, Selman, Alper Gökbulut, and Mehmet Levent Altun. 2025. “Characterization of Phenolic Compounds, Total Phenolic Content and Antioxidant Activity of Three Achillea Species”. Journal of Research in Pharmacy 23 (3): 567-76. https://izlik.org/JA98YT45KE.

EndNote

Şabanoğlu S, Gökbulut A, Altun ML (June 1, 2025) Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. Journal of Research in Pharmacy 23 3 567–576.

IEEE

[1]S. Şabanoğlu, A. Gökbulut, and M. L. Altun, “Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species”, J. Res. Pharm., vol. 23, no. 3, pp. 567–576, June 2025, [Online]. Available: https://izlik.org/JA98YT45KE

ISNAD

Şabanoğlu, Selman - Gökbulut, Alper - Altun, Mehmet Levent. “Characterization of Phenolic Compounds, Total Phenolic Content and Antioxidant Activity of Three Achillea Species”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 567-576. https://izlik.org/JA98YT45KE.

JAMA

1.Şabanoğlu S, Gökbulut A, Altun ML. Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. J. Res. Pharm. 2025;23:567–576.

MLA

Şabanoğlu, Selman, et al. “Characterization of Phenolic Compounds, Total Phenolic Content and Antioxidant Activity of Three Achillea Species”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 567-76, https://izlik.org/JA98YT45KE.

Vancouver

1.Selman Şabanoğlu, Alper Gökbulut, Mehmet Levent Altun. Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):567-76. Available from: https://izlik.org/JA98YT45KE