Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography

Hariyanti Hariyanti; Neng Fisheri Kurniati; Yeyet C Umirtapura; Rachmat Mauludin

Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography

Abstract

Background: cinchonine nanostructured lipid carrier is a preparation with a nanoparticle lipid delivery system. Analysis of levels (efficiency of entrapment) of cinchonine lipid nanostructure is critical to ensure the accuracy of drug dosage. Objective: development and validation of an analytical method for the direct assay of nanostructured lipid carrier’s cinchonine modified by liquid-liquid extraction using high-performance liquid chromatography. Methodology: The research was started by determining the maximum wavelength and validation parameters of selectivity, linearity, sensitivity, precision (intraday and inter days), accuracy, and assay (entrapment efficiency) of nanostructured lipid carrier’s cinchonine directly with modification of liquid-liquid extraction. Results: maximum wavelength of cinchonine 289 nm; selectivity (RF 4.096 ± 0.30 min and TF 1); linearity (R2) 0.9998; sensitivity (LoD 1.2016 mg/L and LoQ 4.0054 mg/L); precision (RSD intraday and RSD inter days <2%); accuracy (recovery 99.52 – 99.87%), entrapment efficiency using direct assay of 94.85 ± 1.91% and indirect assay of 93.35 ± 0.22%. Conclusion: The analysis method for the direct assay of nanostructured lipid carrier’s cinchonine modified by the liquid-liquid extraction method using high-performance liquid chromatography is effective, efficient, and specific with high validity to determine the concentration (entrapment efficiency) of cinchonine from nanostructured lipid carrier’s cinchonine.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biotechnology

Journal Section

Research Article

Authors

Hariyanti Hariyanti
0000-0002-7696-862X
Indonesia

Neng Fisheri Kurniati
0000-0002-3313-6612
Indonesia

Yeyet C Umirtapura This is me
0000-0001-7043-3555
Indonesia

Rachmat Mauludin ^* This is me
0000-0002-3729-0254
Indonesia

Publication Date

June 27, 2025

Submission Date

August 30, 2022

Acceptance Date

October 27, 2022

Published in Issue

Year 2023 Volume: 27 Number: 2

IZ

https://izlik.org/JA73EK42EK

Cite

RIS / Bibtex

APA

Hariyanti, H., Kurniati, N. F., Umirtapura, Y. C., & Mauludin, R. (2025). Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography. Journal of Research in Pharmacy, 27(2), 913-923. https://izlik.org/JA73EK42EK

AMA

1.Hariyanti H, Kurniati NF, Umirtapura YC, Mauludin R. Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography. J. Res. Pharm. 2025;27(2):913-923. https://izlik.org/JA73EK42EK

Chicago

Hariyanti, Hariyanti, Neng Fisheri Kurniati, Yeyet C Umirtapura, and Rachmat Mauludin. 2025. “Development and Validation of an Analytical Method for the Determination of Nanostructured Lipid Carrier’s Cinchonine Used Direct Method Modified by Liquid-Liquid Extraction Using High-Performance Liquid Chromatography”. Journal of Research in Pharmacy 27 (2): 913-23. https://izlik.org/JA73EK42EK.

EndNote

Hariyanti H, Kurniati NF, Umirtapura YC, Mauludin R (June 1, 2025) Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography. Journal of Research in Pharmacy 27 2 913–923.

IEEE

[1]H. Hariyanti, N. F. Kurniati, Y. C. Umirtapura, and R. Mauludin, “Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography”, J. Res. Pharm., vol. 27, no. 2, pp. 913–923, June 2025, [Online]. Available: https://izlik.org/JA73EK42EK

ISNAD

Hariyanti, Hariyanti - Kurniati, Neng Fisheri - Umirtapura, Yeyet C - Mauludin, Rachmat. “Development and Validation of an Analytical Method for the Determination of Nanostructured Lipid Carrier’s Cinchonine Used Direct Method Modified by Liquid-Liquid Extraction Using High-Performance Liquid Chromatography”. Journal of Research in Pharmacy 27/2 (June 1, 2025): 913-923. https://izlik.org/JA73EK42EK.

JAMA

1.Hariyanti H, Kurniati NF, Umirtapura YC, Mauludin R. Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography. J. Res. Pharm. 2025;27:913–923.

MLA

Hariyanti, Hariyanti, et al. “Development and Validation of an Analytical Method for the Determination of Nanostructured Lipid Carrier’s Cinchonine Used Direct Method Modified by Liquid-Liquid Extraction Using High-Performance Liquid Chromatography”. Journal of Research in Pharmacy, vol. 27, no. 2, June 2025, pp. 913-2, https://izlik.org/JA73EK42EK.

Vancouver

1.Hariyanti Hariyanti, Neng Fisheri Kurniati, Yeyet C Umirtapura, Rachmat Mauludin. Development and validation of an analytical method for the determination of nanostructured lipid carrier’s cinchonine used direct method modified by liquid-liquid extraction using high-performance liquid chromatography. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(2):913-2. Available from: https://izlik.org/JA73EK42EK