Preparation and in vitro characterization of lamivudine loaded nanoparticles prepared by acid and/or ester terminated PLGA for effective oral anti-retroviral therapy

A. Alper Öztürk; Gülsel Yurtdaş Kırımlıoğlu

Preparation and in vitro characterization of lamivudine loaded nanoparticles prepared by acid and/or ester terminated PLGA for effective oral anti-retroviral therapy

Abstract

The aim of this study was to formulate Lamivudine (LAM) loaded poly lactic-co-glycolic acid (PLGA) nanoparticle (NP) formulations by ‘double emulsion solvent evaporation’ method for oral administration. PLGA with similar molecular weight but two different chemical end-groups (acid or ester terminated), were used in this study to to compare the effects in the characterization of NPs. Particle size (PS), polydispersity index (PDI), zeta potential, entrapment efficiency (EE%), dissolution and release kinetic studies were carried out for the characterization of NPs and finally for determining the optimum formulation. The selected optimum formulation (B coded) was found to have a PS of 221.00.7 nm and a low PDI as 0.1040.014. Drug EE % of optimal NP formulation was found as 30.280%0.600. In vitro release of LAM loaded NPs were examined in phosphate buffer (pH 6.8). In vitro release studies of LAM-loaded NPs showed an extended release up to 144 hours, thus the demonstration of nanostructures was confirmed. Higuchi and Korsmeyer-Peppas kinetic model was found to fit best for LAM release from PLGA-NPs. The optimum formulation was stable in the 24-hour gastrointestinal stability study and the NP structure was characterized by DSC, FT-IR and 1HNMR analyzes. It could be concluded that LAM loaded NPs seem to be a promising extended release drug delivery system for oral administration in antiretroviral therapy. Lastly, in vitro characterization and release data demonstrated the possibility of improved bioavailability of LAM by PLGA-NP formulation and the effect of polymers used in this study on formulation characteristics were also elucidated

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biotechnology, Pharmaceutical Delivery Technologies

Journal Section

Research Article

Authors

A. Alper Öztürk ^*
Türkiye

Gülsel Yurtdaş Kırımlıoğlu
Türkiye

Publication Date

June 27, 2025

Submission Date

March 4, 2019

Acceptance Date

May 23, 2019

Published in Issue

Year 2019 Volume: 23 Number: 5

IZ

https://izlik.org/JA37JU27YR

Cite

RIS / Bibtex

APA

Öztürk, A. A., & Yurtdaş Kırımlıoğlu, G. (2025). Preparation and in vitro characterization of lamivudine loaded nanoparticles prepared by acid and/or ester terminated PLGA for effective oral anti-retroviral therapy. Journal of Research in Pharmacy, 23(5), 897-913. https://izlik.org/JA37JU27YR

AMA

1.Öztürk AA, Yurtdaş Kırımlıoğlu G. Preparation and in vitro characterization of lamivudine loaded nanoparticles prepared by acid and/or ester terminated PLGA for effective oral anti-retroviral therapy. J. Res. Pharm. 2025;23(5):897-913. https://izlik.org/JA37JU27YR

Chicago

Öztürk, A. Alper, and Gülsel Yurtdaş Kırımlıoğlu. 2025. “Preparation and in Vitro Characterization of Lamivudine Loaded Nanoparticles Prepared by Acid and Or Ester Terminated PLGA for Effective Oral Anti-Retroviral Therapy”. Journal of Research in Pharmacy 23 (5): 897-913. https://izlik.org/JA37JU27YR.

EndNote

Öztürk AA, Yurtdaş Kırımlıoğlu G (July 1, 2025) Preparation and in vitro characterization of lamivudine loaded nanoparticles prepared by acid and/or ester terminated PLGA for effective oral anti-retroviral therapy. Journal of Research in Pharmacy 23 5 897–913.

IEEE

[1]A. A. Öztürk and G. Yurtdaş Kırımlıoğlu, “Preparation and in vitro characterization of lamivudine loaded nanoparticles prepared by acid and/or ester terminated PLGA for effective oral anti-retroviral therapy”, J. Res. Pharm., vol. 23, no. 5, pp. 897–913, July 2025, [Online]. Available: https://izlik.org/JA37JU27YR

ISNAD

Öztürk, A. Alper - Yurtdaş Kırımlıoğlu, Gülsel. “Preparation and in Vitro Characterization of Lamivudine Loaded Nanoparticles Prepared by Acid and Or Ester Terminated PLGA for Effective Oral Anti-Retroviral Therapy”. Journal of Research in Pharmacy 23/5 (July 1, 2025): 897-913. https://izlik.org/JA37JU27YR.

JAMA

1.Öztürk AA, Yurtdaş Kırımlıoğlu G. Preparation and in vitro characterization of lamivudine loaded nanoparticles prepared by acid and/or ester terminated PLGA for effective oral anti-retroviral therapy. J. Res. Pharm. 2025;23:897–913.

MLA

Öztürk, A. Alper, and Gülsel Yurtdaş Kırımlıoğlu. “Preparation and in Vitro Characterization of Lamivudine Loaded Nanoparticles Prepared by Acid and Or Ester Terminated PLGA for Effective Oral Anti-Retroviral Therapy”. Journal of Research in Pharmacy, vol. 23, no. 5, July 2025, pp. 897-13, https://izlik.org/JA37JU27YR.

Vancouver

1.A. Alper Öztürk, Gülsel Yurtdaş Kırımlıoğlu. Preparation and in vitro characterization of lamivudine loaded nanoparticles prepared by acid and/or ester terminated PLGA for effective oral anti-retroviral therapy. J. Res. Pharm. [Internet]. 2025 Jul. 1;23(5):897-913. Available from: https://izlik.org/JA37JU27YR