Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector

Zeynep Filiz; Tulay Oymak; Emrah Dural

Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector

Abstract

This study aimed at developing and validating a reversed-phase (RP) high-performance liquid chromatography (HPLC) method, for simultaneous determination of five synthetic dyes called tartrazine (TRZ), sunset yellow (SY), allura red AC (AR), brilliant blue FCF (BB) and erythrosine B (EB) in cosmetic samples. The separation was performed by a C18 reverse phase analytical column (4.6 x 250 mm, 5 µm) at 30°C with gradient elution and the mobile phase contained 20 mM ammonium acetate buffer, acetonitrile and methanol. Flow was 1.0 mL/min. Detection wavelengths of diode array detector (DAD) were set at 420, 480, 510, 634 and 530 nm for TRZ, SY, AR, BB and EB, respectively. The dyes were analysed in 24 min. The limits of detection (LOD) was ≤ 0.18 µg/mL. The recovery was between 88.7 and 103.0%. Precision was ≤ .7.33 (RSD%) and accuracy was ≤ 3.0 (RE%). It was determined that 7 different cosmetic samples analyzed, consisting of soap, shower gel, eyeshadow, mouthwash, and lip pencil contained synthetic dyes at a concentration of 0.29 to 10.81 mg/g.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Toxicology

Journal Section

Research Article

Authors

Zeynep Filiz This is me
Türkiye

Tulay Oymak
Türkiye

Emrah Dural ^*
Türkiye

Publication Date

June 27, 2025

Submission Date

May 26, 2019

Acceptance Date

October 6, 2019

Published in Issue

Year 2019 Volume: 23 Number: 6

IZ

https://izlik.org/JA73GX55BX

Cite

RIS / Bibtex

APA

Filiz, Z., Oymak, T., & Dural, E. (2025). Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector. Journal of Research in Pharmacy, 23(6), 1048-1059. https://izlik.org/JA73GX55BX

AMA

1.Filiz Z, Oymak T, Dural E. Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector. J. Res. Pharm. 2025;23(6):1048-1059. https://izlik.org/JA73GX55BX

Chicago

Filiz, Zeynep, Tulay Oymak, and Emrah Dural. 2025. “Determination of Synthetic Colorants in Cosmetic Products by Reversed-Phase High-Performance Liquid Chromatography Coupled With Diode-Array Detector”. Journal of Research in Pharmacy 23 (6): 1048-59. https://izlik.org/JA73GX55BX.

EndNote

Filiz Z, Oymak T, Dural E (July 1, 2025) Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector. Journal of Research in Pharmacy 23 6 1048–1059.

IEEE

[1]Z. Filiz, T. Oymak, and E. Dural, “Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector”, J. Res. Pharm., vol. 23, no. 6, pp. 1048–1059, July 2025, [Online]. Available: https://izlik.org/JA73GX55BX

ISNAD

Filiz, Zeynep - Oymak, Tulay - Dural, Emrah. “Determination of Synthetic Colorants in Cosmetic Products by Reversed-Phase High-Performance Liquid Chromatography Coupled With Diode-Array Detector”. Journal of Research in Pharmacy 23/6 (July 1, 2025): 1048-1059. https://izlik.org/JA73GX55BX.

JAMA

1.Filiz Z, Oymak T, Dural E. Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector. J. Res. Pharm. 2025;23:1048–1059.

MLA

Filiz, Zeynep, et al. “Determination of Synthetic Colorants in Cosmetic Products by Reversed-Phase High-Performance Liquid Chromatography Coupled With Diode-Array Detector”. Journal of Research in Pharmacy, vol. 23, no. 6, July 2025, pp. 1048-59, https://izlik.org/JA73GX55BX.

Vancouver

1.Zeynep Filiz, Tulay Oymak, Emrah Dural. Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector. J. Res. Pharm. [Internet]. 2025 Jul. 1;23(6):1048-59. Available from: https://izlik.org/JA73GX55BX