The effects of Origanum onites in streptozotocin-induced diabetes mellitus in rats

Abstract

Diabetes mellitus is a chronic disease characterized by decreased insulin synthesis and/or increased blood glucose due to insulin resistance. In this study, streptozotocin (STZ)-induced diabetic rats were evaluated and it was aimed to investigate the protective effects of Origanum onites L. (OO) against possible changes in these parameters. Male Sprague-Dawley rats weighing 300-400g were divided into three groups control (C), diabetes mellitus (DM) and diabetes mellitus + OO (OO group). DM was induced by administration of STZ 60 mg/kg dose intraperitoneally (i.p.) after 48 hours, rats with blood glucose values higher than 200 mg/dL were considered DM. Origanum extract was administered i.p. to the OO group at 50 mg/kg per day for 6 weeks. Serum AST, ALT, creatinine, and inflammatory cytokine levels were measured. MDA and GSH levels were measured in liver tissues. AST, ALT, creatinine, and MDA levels were found to be increased in the DM group, while a significant decrease in these levels was observed in the treated group. While GSH values fell in the DM group, a significant increase occurred in the OO group (n=6, p<0.0001; two-way ANOVA). When the plasma levels of cytokines were examined, an increase was observed in the DM group and a significant decrease was observed in the OO group. When we evaluate our findings, we think that OO has a protective effect against complications that may occur in DM by preventing oxidant damage and inflammation. Further studies are needed on the protective effects of OO in DM.

Keywords

• Diabetes mellitus
• oxidative stress
• liver
• Origanum onites
• inflammatory cytokine

References

1. [1] Tao Z, Shi A, Zhao J. Epidemiological Perspectives of Diabetes. Cell Biochem Biophys. 2015; 73(1): 181-5. [CrossRef]
2. [2] Cole JB, Florez JC. Genetics of diabetes mellitus and diabetes complications. Nat Rev Nephrol. 2020; 16(7): 377-90. [CrossRef]
3. [3] Iminger-Finger I, Kargul J, Laurent GJ. Diabetes: Present and future. Int J Biochem Cell Biol. 2017; 88: 196. [CrossRef]
4. [4] Ando K, Fujita T. Metabolic syndrome and oxidative stress. Free Radic Biol Med. 2009; 47(3): 213-8. [CrossRef]
5. [5] Tan SY, Mei Wong JL, Sim YJ, Wong SS, Mohamed Elhassan SA, Tan SH, et al. Type 1 and 2 diabetes mellitus: A review on current treatment approach and gene therapy as potential intervention. Diabetes Metab Syndr. 2019; 13(1): 364-72. [CrossRef]
6. [6] Pivari F, Mingione A, Brasacchio C, Soldati L. Curcumin and Type 2 Diabetes Mellitus: Prevention and Treatment. Nutrients. 2019; 11(8). [CrossRef]
7. [7] Ozdemir N, Ozgen Y, Kiralan M, Bayrak A, Arslan N, Ramadan MF. Effect of different drying methods on the essential oil yield, composition and antioxidant activity of Origanum vulgare L. and Origanum onites L. Journal of Food Measurement and Characterization. 2018; 12(2): 820-5. [CrossRef]
8. [8] Altintas A, Tabanca N, Tyihák E, Ott PG, Móricz AM, Mincsovics E, et al. Characterization of volatile constituents from Origanum onites and their antifungal and antibacterial activity. J AOAC Int. 2013; 96(6): 1200-8. [CrossRef]

9. [9] Karstoft K, Pedersen BK. Exercise and type 2 diabetes: focus on metabolism and inflammation. Immunol Cell Biol. 2016; 94(2): 146-50. [CrossRef]
10. [10] Neumann C, Scheffold A, Rutz S. Functions and regulation of T cell-derived interleukin-10. Semin Immunol. 2019; 44: 101344. [CrossRef]
11. [11] Schwarz S, Mrosewski I, Silawal S, Schulze-Tanzil G. The interrelation of osteoarthritis and diabetes mellitus: considering the potential role of interleukin-10 and in vitro models for further analysis. Inflamm Res. 2018; 67(4): 285-300. [CrossRef]
12. [12] Asadikaram G, Ram M, Izadi A, Sheikh Fathollahi M, Nematollahi MH, Najafipour H, et al. The study of the serum level of IL-4, TGF-β, IFN-γ, and IL-6 in overweight patients with and without diabetes mellitus and hypertension. J Cell Biochem. 2019; 120(3): 4147-57. [CrossRef]
13. [13] Richardson A, Park WG. Acute pancreatitis and diabetes mellitus: a review. Korean J Intern Med. 2021; 36(1): 15-24. [CrossRef]
14. [14] Wang L, Peng W, Zhao Z, Zhang M, Shi Z, Song Z, et al. Prevalence and Treatment of Diabetes in China, 2013-2018. Jama. 2021; 326(24): 2498-506. [CrossRef]
15. [15] Rajasekaran S, Sivagnanam K, Ravi K, Subramanian S. Hypoglycemic effect of Aloe vera gel on streptozotocin-induced diabetes in experimental rats. J Med Food. 2004; 7(1): 61-6. [CrossRef]
16. [16] Schmidt AM. Highlighting Diabetes Mellitus: The Epidemic Continues. Arterioscler Thromb Vasc Biol. 2018; 38(1): e1-e8. [CrossRef]
17. [17] Aouacheri O, Saka S, Krim M, Messaadia A, Maidi I. The investigation of the oxidative stress-related parameters in type 2 diabetes mellitus. Can J Diabetes. 2015; 39(1): 44-9. [CrossRef]
18. [18] Banerjee M, Saxena M. Interleukin-1 (IL-1) family of cytokines: role in type 2 diabetes. Clin Chim Acta. 2012; 413(15-16): 1163-70. [CrossRef]
19. [19] Cabrera SM, Henschel AM, Hessner MJ. Innate inflammation in type 1 diabetes. Transl Res. 2016; 167(1): 214-27. [CrossRef]
20. [20] Moussa H, Quezada E, Viña D, Riadi H, Gil-Longo J. Redox-Active Phenolic Compounds Mediate the Cytotoxic and Antioxidant Effects of Carpodesmia tamariscifolia (=Cystoseira tamariscifolia). Chem Biodivers. 2020; 17(7): e2000121. [CrossRef]
21. [21] Ozkan G, Kamiloglu S, Ozdal T, Boyacioglu D, Capanoglu E. Potential Use of Turkish Medicinal Plants in the Treatment of Various Diseases. Molecules. 2016; 21(3): 257. [CrossRef]
22. [22] Ocaña-Fuentes A, Arranz-Gutiérrez E, Señorans FJ, Reglero G. Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: anti-inflammatory properties based on cytokine response on THP-1 macrophages. Food Chem Toxicol. 2010; 48(6): 1568-75. [CrossRef]
23. [23] Ceriello A. The emerging challenge in diabetes: the "metabolic memory". Vascul Pharmacol. 2012; 57(5-6): 133-8. [CrossRef]
24. [24] Sharma R, Rao R, Kumar S, Mahant S, Khatkar S. Therapeutic Potential of Citronella Essential Oil: A Review. Curr Drug Discov Technol. 2019; 16(4): 330-9. [CrossRef]
25. [25] Lermioglu F, Bagci S, Onderoglu S, Ortac R, Tugrul L. Evaluation of the long-term effects of oleum origani on the toxicity induced by administration of streptozotocin in rats. J Pharm Pharmacol. 1997; 49(11): 1157-61. [CrossRef]
26. [26] el-Ashmawy IM, el-Nahas AF, Salama OM. Protective effect of volatile oil, alcoholic and aqueous extracts of Origanum majorana on lead acetate toxicity in mice. Basic Clin Pharmacol Toxicol. 2005; 97(4): 238-43. [CrossRef]
27. [27] Rehman K, Akash MSH, Liaqat A, Kamal S, Qadir MI, Rasul A. Role of Interleukin-6 in Development of Insulin Resistance and Type 2 Diabetes Mellitus. Crit Rev Eukaryot Gene Expr. 2017; 27(3): 229-36. [CrossRef]
28. [28] Barry JC, Shakibakho S, Durrer C, Simtchouk S, Jawanda KK, Cheung ST, et al. Hyporesponsiveness to the anti-inflammatory action of interleukin-10 in type 2 diabetes. Sci Rep. 2016; 6: 21244. [CrossRef]
29. [29] Davoodi-Semiromi A, Yang JJ, She JX. IL-12p40 is associated with type 1 diabetes in Caucasian-American families. Diabetes. 2002; 51(7): 2334-6. [CrossRef]