The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells

Inci Kazkayası; Merve Denizaltı

The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells

Abstract

Chronic wounds in diabetes are common and serious health problems which also create significant economic burden in society. A comprehensive treatment has not yet been found in chronic wounds and new treatment recommendations are needed. The beneficial effects of metformin on wound healing was shown previously in healthy or diabetic animal models. Hyaluronic acid was also found to accelerate wound closure in clinical trials as well as animal models. Metformin and hyaluronic acid have been shown to be beneficial for wound healing in diabetes in in vivo studies, but their effect on wound healing under in vitro diabetic conditions have not yet been enlightened. In this study; we investigated the effects of metformin and hyaluronic acid on wound healing in mouse fibroblast cells under high glucose conditions. We used the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and scratch migration assays to examine fibroblast cell viability and migration in wound healing respectively. We showed that metformin and hyaluronic acid enhanced wound healing in fibroblasts incubated with high glucose by increasing cell viability, proliferation and migration. This is the first study to examine the effect of metformin, hyaluronic acid, or their combination on wound healing under high glucose conditions in vitro.

Keywords

References

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Inci Kazkayası ^*
0000-0003-1159-9680
Türkiye

Merve Denizaltı
0000-0001-5363-606X
Türkiye

Publication Date

June 28, 2025

Submission Date

March 29, 2023

Acceptance Date

May 10, 2023

Published in Issue

Year 2023 Volume: 27 Number: 4

IZ

https://izlik.org/JA76TA48FC

Cite

RIS / Bibtex

APA

Kazkayası, I., & Denizaltı, M. (2025). The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells. Journal of Research in Pharmacy, 27(4), 1338-1345. https://izlik.org/JA76TA48FC

AMA

1.Kazkayası I, Denizaltı M. The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells. J. Res. Pharm. 2025;27(4):1338-1345. https://izlik.org/JA76TA48FC

Chicago

Kazkayası, Inci, and Merve Denizaltı. 2025. “The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells”. Journal of Research in Pharmacy 27 (4): 1338-45. https://izlik.org/JA76TA48FC.

EndNote

Kazkayası I, Denizaltı M (July 1, 2025) The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells. Journal of Research in Pharmacy 27 4 1338–1345.

IEEE

[1]I. Kazkayası and M. Denizaltı, “The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells”, J. Res. Pharm., vol. 27, no. 4, pp. 1338–1345, July 2025, [Online]. Available: https://izlik.org/JA76TA48FC

ISNAD

Kazkayası, Inci - Denizaltı, Merve. “The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells”. Journal of Research in Pharmacy 27/4 (July 1, 2025): 1338-1345. https://izlik.org/JA76TA48FC.

JAMA

1.Kazkayası I, Denizaltı M. The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells. J. Res. Pharm. 2025;27:1338–1345.

MLA

Kazkayası, Inci, and Merve Denizaltı. “The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells”. Journal of Research in Pharmacy, vol. 27, no. 4, July 2025, pp. 1338-45, https://izlik.org/JA76TA48FC.

Vancouver

1.Inci Kazkayası, Merve Denizaltı. The Effects of Metformin and Hyaluronic Acid on Wound Healing in High Glucose Incubated Fibroblast Cells. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(4):1338-45. Available from: https://izlik.org/JA76TA48FC