The ameliorating effect of silymarin against vancomycin induced apoptosis and inflammation in rat liver

Sevda Güzel Kara; Zuhal Uçkun Şahinoğulları; Necmiye Canacankatan; Şerife Efsun Antmen; Deniz Kibar; Gulsen Bayrak

The ameliorating effect of silymarin against vancomycin induced apoptosis and inflammation in rat liver

Abstract

Silymarin (SL), a flavonolignan complex isolated from seeds of Silybum marianum (Asteraceae), is known for its hepatoprotective, anti-apoptotic, anti-inflammatory, and antioxidant activities. A glycopeptide antibiotic, Vancomycin (VA) which is used for the treatment of serious infections caused by multi-resistant Gram-positive microorganisms has been clinically used for a long time. The aim of the present study was to evaluate potential therapeutic efficiency of SL against VA-induced apoptosis and inflammation using apoptotic (caspase-3, -8, and, -9 enzyme activities) and inflammatory (Tumor necrosis factor-alpha (TNF-α)) markers, and histopathological examinations in rat liver. A total of 49 male Wistar albino rats was divided into 7 groups including control (saline, intraperitoneally (i.p.)), Dimethyl sulfoxide (i.p.), VA (400 mg/kg/day, i.p.), SL100 (100 mg/kg/day, i.p.), VA+SL50 (50 mg/kg/day, i.p.), VA+SL100 (100 mg/kg/day, i.p.), and VA+SL200 (200 mg/kg/day, i.p.). SL was administered once a daily for 8 days. One day after the first treatment of SL, VA administration was started and continued for 7 days. Hepatic TNF-α levels were evaluated by ELISA and hepatic caspase activities were evaluated according to the colorimetric method. Significantly increased caspase activities were determined in VA group compared to control group (P<0.05). However, significantly reduced caspase activities were determined in VA+SL200 group when compared to VA group (P<0.05). TNF-α level in VA group was found to be statistically higher than control group (P<0.05). TNF-α levels of SL100, VA+SL (50, 100, and 200) groups were significantly lower than VA group (P<0.05). VA+SL200 group was found to be the most effective group according to reducing caspase activities and TNF-α level.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry, Pharmacognosy, Pharmaceutical Toxicology

Journal Section

Research Article

Authors

Sevda Güzel Kara ^*
Türkiye

Zuhal Uçkun Şahinoğulları
Türkiye

Necmiye Canacankatan
Türkiye

Şerife Efsun Antmen This is me
Türkiye

Deniz Kibar
Türkiye

Gulsen Bayrak
Türkiye

Publication Date

June 27, 2025

Submission Date

January 1, 2019

Acceptance Date

February 24, 2019

Published in Issue

Year 2019 Volume: 23 Number: 4

IZ

https://izlik.org/JA33KM93BS

Cite

RIS / Bibtex

APA

Güzel Kara, S., Uçkun Şahinoğulları, Z., Canacankatan, N., Antmen, Ş. E., Kibar, D., & Bayrak, G. (2025). The ameliorating effect of silymarin against vancomycin induced apoptosis and inflammation in rat liver. Journal of Research in Pharmacy, 23(4), 719-728. https://izlik.org/JA33KM93BS

AMA

1.Güzel Kara S, Uçkun Şahinoğulları Z, Canacankatan N, Antmen ŞE, Kibar D, Bayrak G. The ameliorating effect of silymarin against vancomycin induced apoptosis and inflammation in rat liver. J. Res. Pharm. 2025;23(4):719-728. https://izlik.org/JA33KM93BS

Chicago

Güzel Kara, Sevda, Zuhal Uçkun Şahinoğulları, Necmiye Canacankatan, Şerife Efsun Antmen, Deniz Kibar, and Gulsen Bayrak. 2025. “The Ameliorating Effect of Silymarin Against Vancomycin Induced Apoptosis and Inflammation in Rat Liver”. Journal of Research in Pharmacy 23 (4): 719-28. https://izlik.org/JA33KM93BS.

EndNote

Güzel Kara S, Uçkun Şahinoğulları Z, Canacankatan N, Antmen ŞE, Kibar D, Bayrak G (June 1, 2025) The ameliorating effect of silymarin against vancomycin induced apoptosis and inflammation in rat liver. Journal of Research in Pharmacy 23 4 719–728.

IEEE

[1]S. Güzel Kara, Z. Uçkun Şahinoğulları, N. Canacankatan, Ş. E. Antmen, D. Kibar, and G. Bayrak, “The ameliorating effect of silymarin against vancomycin induced apoptosis and inflammation in rat liver”, J. Res. Pharm., vol. 23, no. 4, pp. 719–728, June 2025, [Online]. Available: https://izlik.org/JA33KM93BS

ISNAD

Güzel Kara, Sevda - Uçkun Şahinoğulları, Zuhal - Canacankatan, Necmiye - Antmen, Şerife Efsun - Kibar, Deniz - Bayrak, Gulsen. “The Ameliorating Effect of Silymarin Against Vancomycin Induced Apoptosis and Inflammation in Rat Liver”. Journal of Research in Pharmacy 23/4 (June 1, 2025): 719-728. https://izlik.org/JA33KM93BS.

JAMA

1.Güzel Kara S, Uçkun Şahinoğulları Z, Canacankatan N, Antmen ŞE, Kibar D, Bayrak G. The ameliorating effect of silymarin against vancomycin induced apoptosis and inflammation in rat liver. J. Res. Pharm. 2025;23:719–728.

MLA

Güzel Kara, Sevda, et al. “The Ameliorating Effect of Silymarin Against Vancomycin Induced Apoptosis and Inflammation in Rat Liver”. Journal of Research in Pharmacy, vol. 23, no. 4, June 2025, pp. 719-28, https://izlik.org/JA33KM93BS.

Vancouver

1.Sevda Güzel Kara, Zuhal Uçkun Şahinoğulları, Necmiye Canacankatan, Şerife Efsun Antmen, Deniz Kibar, Gulsen Bayrak. The ameliorating effect of silymarin against vancomycin induced apoptosis and inflammation in rat liver. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(4):719-28. Available from: https://izlik.org/JA33KM93BS