Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities

Rima Konya Konuk; Rengin Reis; Hande Sipahi; Anita Barta; Judit Hohmann; Hasan Kırmızıbekmez

Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities

Abstract

Using various chromatographic methods, seven compounds, including two secoiridoid glycosides; depressine (1) and gentiopicroside (2), one iridoid glycoside; loganic acid (3), two flavone-C-glycosides; isoorientin (4) and isovitexin (5), one xanthone-C-glycoside, mangiferin (6) as well as a nucleoside; adenosine (7) were isolated from the MeOH extract prepared from the aerial parts of Gentiana asclepiadea L. Their structures were elucidated unambiguously by spectroscopic methods such as 1D and 2D NMR as well as HRESIMS. The anti-inflammatory and analgesic activities of the isolated compounds were also evaluated in vitro. Among the tested compounds, 1, 2, and 4 showed potent anti-inflammatory activity through both nitrite and IL-6 pathways at 200 µM. Besides, compound 1 exhibited the highest decrease in PGE2 level, with a higher inhibition rate compared to positive control indomethacin.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Rima Konya Konuk ^*
0000-0001-6228-4369
Türkiye

Rengin Reis
0000-0002-3484-2201
Türkiye

Hande Sipahi
0000-0001-6482-3143
Türkiye

Anita Barta This is me
Hungary

Judit Hohmann This is me
0000-0002-2887-6392
Hungary

Hasan Kırmızıbekmez
0000-0002-6118-8225
Türkiye

Publication Date

June 28, 2025

Submission Date

December 21, 2022

Acceptance Date

February 2, 2023

Published in Issue

Year 2023 Volume: 27 Number: 3

IZ

https://izlik.org/JA82YP59JJ

Cite

RIS / Bibtex

APA

Konya Konuk, R., Reis, R., Sipahi, H., Barta, A., Hohmann, J., & Kırmızıbekmez, H. (2025). Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities. Journal of Research in Pharmacy, 27(3), 1202-1212. https://izlik.org/JA82YP59JJ

AMA

1.Konya Konuk R, Reis R, Sipahi H, Barta A, Hohmann J, Kırmızıbekmez H. Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities. J. Res. Pharm. 2025;27(3):1202-1212. https://izlik.org/JA82YP59JJ

Chicago

Konya Konuk, Rima, Rengin Reis, Hande Sipahi, Anita Barta, Judit Hohmann, and Hasan Kırmızıbekmez. 2025. “Iridoids and Flavonoids from the Aerial Parts of Gentiana Asclepiadea L. With Anti-Inflammatory and Analgesic Activities”. Journal of Research in Pharmacy 27 (3): 1202-12. https://izlik.org/JA82YP59JJ.

EndNote

Konya Konuk R, Reis R, Sipahi H, Barta A, Hohmann J, Kırmızıbekmez H (June 1, 2025) Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities. Journal of Research in Pharmacy 27 3 1202–1212.

IEEE

[1]R. Konya Konuk, R. Reis, H. Sipahi, A. Barta, J. Hohmann, and H. Kırmızıbekmez, “Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities”, J. Res. Pharm., vol. 27, no. 3, pp. 1202–1212, June 2025, [Online]. Available: https://izlik.org/JA82YP59JJ

ISNAD

Konya Konuk, Rima - Reis, Rengin - Sipahi, Hande - Barta, Anita - Hohmann, Judit - Kırmızıbekmez, Hasan. “Iridoids and Flavonoids from the Aerial Parts of Gentiana Asclepiadea L. With Anti-Inflammatory and Analgesic Activities”. Journal of Research in Pharmacy 27/3 (June 1, 2025): 1202-1212. https://izlik.org/JA82YP59JJ.

JAMA

1.Konya Konuk R, Reis R, Sipahi H, Barta A, Hohmann J, Kırmızıbekmez H. Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities. J. Res. Pharm. 2025;27:1202–1212.

MLA

Konya Konuk, Rima, et al. “Iridoids and Flavonoids from the Aerial Parts of Gentiana Asclepiadea L. With Anti-Inflammatory and Analgesic Activities”. Journal of Research in Pharmacy, vol. 27, no. 3, June 2025, pp. 1202-1, https://izlik.org/JA82YP59JJ.

Vancouver

1.Rima Konya Konuk, Rengin Reis, Hande Sipahi, Anita Barta, Judit Hohmann, Hasan Kırmızıbekmez. Iridoids and flavonoids from the aerial parts of Gentiana asclepiadea L. with anti-inflammatory and analgesic activities. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(3):1202-1. Available from: https://izlik.org/JA82YP59JJ