Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel

Adeltrudis Adelsa Danimayostu; Endang Lukitaningsih; Ronny Martien; Retno Danarti

Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel

Abstract

High Performance Liquid Chromatography (HPLC) was used to determine vitamin D3 content from the topical dosage form. Self-nanoemulsifying drug delivery systems (SNEDDS) based hydrogel was aimed to optimize the delivery of vitamin D3 through the skin due to its high lipophilicity. Quantifying vitamin D3 was performed using the internal standard, vitamin D2, on LiChrocart C18 125-4 reverse phase column. Several characteristics of validation were referred including specificity, linearity, range, accuracy, precision, limits of detection, and quantification. The mobile phase was optimized as a mixture of acetonitrile-methanol-water for injection 94:3:3 v/v, and the flow rate was optimized at 0.8 mL/min at 25°C. Vitamin D3 detection and quantification were observed at a wavelength of 266 nm using an ultra-violet (UV) detector. The analysis method performed no excipient interference, including separating vitamin D3 from its matrix. Extraction of vitamin D3 from its matrix was observed using the dialysis membrane, with agitation (vortex) and no agitation (immersion). The drug content determination was found to be 108.22 % ± 14.77% for the immersion method and 72.04% ± 33.37% for the vortex method. Further, the high variability of the results indicated the dialysis membrane was not a suitable method for determining lipophilic drug content from the colloidal polymer matrix. The other techniques with negligible variation, such as reverse dialysis or subsequent solvent addition, are preferred.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Adeltrudis Adelsa Danimayostu This is me
0000-0003-4642-5330
Indonesia

Endang Lukitaningsih ^*
0000-0002-4969-5128
Indonesia

Ronny Martien
0000-0001-7291-6497
Indonesia

Retno Danarti
0000-0001-7594-184X
Indonesia

Publication Date

June 28, 2025

Submission Date

December 1, 2022

Acceptance Date

February 6, 2023

Published in Issue

Year 2023 Volume: 27 Number: 3

IZ

https://izlik.org/JA23DL97PG

Cite

RIS / Bibtex

APA

Danimayostu, A. A., Lukitaningsih, E., Martien, R., & Danarti, R. (2025). Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel. Journal of Research in Pharmacy, 27(3), 1213-1219. https://izlik.org/JA23DL97PG

AMA

1.Danimayostu AA, Lukitaningsih E, Martien R, Danarti R. Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel. J. Res. Pharm. 2025;27(3):1213-1219. https://izlik.org/JA23DL97PG

Chicago

Danimayostu, Adeltrudis Adelsa, Endang Lukitaningsih, Ronny Martien, and Retno Danarti. 2025. “Determination of Vitamin D3 Loaded Self- Nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel”. Journal of Research in Pharmacy 27 (3): 1213-19. https://izlik.org/JA23DL97PG.

EndNote

Danimayostu AA, Lukitaningsih E, Martien R, Danarti R (June 1, 2025) Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel. Journal of Research in Pharmacy 27 3 1213–1219.

IEEE

[1]A. A. Danimayostu, E. Lukitaningsih, R. Martien, and R. Danarti, “Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel”, J. Res. Pharm., vol. 27, no. 3, pp. 1213–1219, June 2025, [Online]. Available: https://izlik.org/JA23DL97PG

ISNAD

Danimayostu, Adeltrudis Adelsa - Lukitaningsih, Endang - Martien, Ronny - Danarti, Retno. “Determination of Vitamin D3 Loaded Self- Nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel”. Journal of Research in Pharmacy 27/3 (June 1, 2025): 1213-1219. https://izlik.org/JA23DL97PG.

JAMA

1.Danimayostu AA, Lukitaningsih E, Martien R, Danarti R. Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel. J. Res. Pharm. 2025;27:1213–1219.

MLA

Danimayostu, Adeltrudis Adelsa, et al. “Determination of Vitamin D3 Loaded Self- Nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel”. Journal of Research in Pharmacy, vol. 27, no. 3, June 2025, pp. 1213-9, https://izlik.org/JA23DL97PG.

Vancouver

1.Adeltrudis Adelsa Danimayostu, Endang Lukitaningsih, Ronny Martien, Retno Danarti. Determination of Vitamin D3 Loaded Self- nanoemulsifying Drug Delivery Systems (SNEDDS) Based Hydrogel. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(3):1213-9. Available from: https://izlik.org/JA23DL97PG