The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L.

Selma Sezen; Muhammed Sait Ertuğrul; Cemil Bayram; Mustafa Özkaraca; Taha Yasin Koç; Abdussamed Yasin Demir; Medine Güllüce; Ahmet Hacımüftüoğlu

The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L.

Abstract

Products from Mentha species are widely used in medical applications, including wound healing, due to the bioactive compounds they may contain. However, data on the effect of Mentha longifolia L. on wound healing are limited. This study investigated the antimicrobial and wound healing-promoting effects of Mentha longifolia L. essential oil using in vitro methods. The chemical compositions of the essential oil were identified using gas chromatography/mass spectrometry, while the agar well diffusion and disk diffusion methods were used to determine antimicrobial activity against pathogenic strains. A scratch wound healing assay was performed following determination of the cytotoxic dose in human fibroblast cell lines. The media were used for biochemical analysis 48 h after the in vitro wound model. Immunohistochemical staining was applied to determine the contribution of the essential oil to wound healing. The essential oil exhibited varying levels of antimicrobial activity against the tested pathogens and increased cell viability. All doses in the scratch wound healing assay promoted wound closure in a shorter time than in the control group. TAC levels were higher in the treated groups than in the control group, while TOS, IL-6, and TNF-α levels were lower. Levels of expression of FGF 2, IGF, and TGF-β were higher in the treated groups than in the control group at increasing doses. The essential oil of Mentha longifolia L. exhibited antimicrobial effects and improved wound healing at doses of 5 µg/mL and 10 µg/mL

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Selma Sezen ^*
0000-0001-6575-6149
Türkiye

Muhammed Sait Ertuğrul
0000-0002-7885-5645
Türkiye

Cemil Bayram
0000-0001-8940-8560
Türkiye

Mustafa Özkaraca This is me
Türkiye

Taha Yasin Koç This is me
Türkiye

Abdussamed Yasin Demir This is me
Türkiye

Medine Güllüce This is me
Türkiye

Ahmet Hacımüftüoğlu
0000-0002-9658-3313
Türkiye

Publication Date

June 28, 2025

Submission Date

November 1, 2022

Acceptance Date

February 4, 2023

Published in Issue

Year 2023 Volume: 27 Number: 3

IZ

https://izlik.org/JA56BU73JD

Cite

RIS / Bibtex

APA

Sezen, S., Ertuğrul, M. S., Bayram, C., Özkaraca, M., Koç, T. Y., Demir, A. Y., Güllüce, M., & Hacımüftüoğlu, A. (2025). The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L. Journal of Research in Pharmacy, 27(3), 1220-1233. https://izlik.org/JA56BU73JD

AMA

1.Sezen S, Ertuğrul MS, Bayram C, et al. The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L. J. Res. Pharm. 2025;27(3):1220-1233. https://izlik.org/JA56BU73JD

Chicago

Sezen, Selma, Muhammed Sait Ertuğrul, Cemil Bayram, et al. 2025. “The In-Vitro Wound Healing Potential of Essential Oil Extracted from Mentha Longifolia L”. Journal of Research in Pharmacy 27 (3): 1220-33. https://izlik.org/JA56BU73JD.

EndNote

Sezen S, Ertuğrul MS, Bayram C, Özkaraca M, Koç TY, Demir AY, Güllüce M, Hacımüftüoğlu A (June 1, 2025) The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L. Journal of Research in Pharmacy 27 3 1220–1233.

IEEE

[1]S. Sezen et al., “The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L”., J. Res. Pharm., vol. 27, no. 3, pp. 1220–1233, June 2025, [Online]. Available: https://izlik.org/JA56BU73JD

ISNAD

Sezen, Selma - Ertuğrul, Muhammed Sait - Bayram, Cemil - Özkaraca, Mustafa - Koç, Taha Yasin - Demir, Abdussamed Yasin - Güllüce, Medine - Hacımüftüoğlu, Ahmet. “The In-Vitro Wound Healing Potential of Essential Oil Extracted from Mentha Longifolia L”. Journal of Research in Pharmacy 27/3 (June 1, 2025): 1220-1233. https://izlik.org/JA56BU73JD.

JAMA

1.Sezen S, Ertuğrul MS, Bayram C, Özkaraca M, Koç TY, Demir AY, Güllüce M, Hacımüftüoğlu A. The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L. J. Res. Pharm. 2025;27:1220–1233.

MLA

Sezen, Selma, et al. “The In-Vitro Wound Healing Potential of Essential Oil Extracted from Mentha Longifolia L”. Journal of Research in Pharmacy, vol. 27, no. 3, June 2025, pp. 1220-33, https://izlik.org/JA56BU73JD.

Vancouver

1.Selma Sezen, Muhammed Sait Ertuğrul, Cemil Bayram, Mustafa Özkaraca, Taha Yasin Koç, Abdussamed Yasin Demir, Medine Güllüce, Ahmet Hacımüftüoğlu. The in-vitro Wound Healing Potential of Essential Oil Extracted from Mentha longifolia L. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(3):1220-33. Available from: https://izlik.org/JA56BU73JD