Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells

Ecem Kaya Sezginer; Omer Faruk Kırlangıç; Cengiz Karakaya; Sema Ören; Tuğba Fatsa; Taner Özgürtaş

Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells

Abstract

This study investigated the apoptotic effects of a histone deacetylase (HDAC) inhibitor, sodium butyrate (NaBu) and cisplatin as monotherapy or in combination on SH-SY5Y human neuroblastoma cells. MTT and colony formation assays were performed to investigate the anti-proliferative effects of NaBu in combination with cisplatin. Analysis of SH-SY5Y cell apoptosis was performed by Annexin V-FITC/PI and DAPI staining. The gene and protein expressions were measured by RT-PCR and Western blot. The cytotoxic effect of the combined treatment was higher than single treatments, while the combination of two did not show much higher inhibition of colony formation and induction of apoptosis compared to cisplatin alone. Treatment with cisplatin upregulated the expression of Bax and Bcl-2 and combination treatment enhanced Bax gene expression and Bax/Bcl-2 ratio. NaBu alone and combined with cisplatin enhanced the expression of nuclear factor kappa B (NF-κB) protein, however cisplatin alone significantly decreased NFκB protein levels. p53 and phosphorylated inhibitor kappa B alpha (p-IκBα) expression did not alter following any of the treatments. The combination of NaBu with cisplatin did not show any additional benefit in SH-SY5Y human neuroblastoma cells compared to cisplatin as a conventional chemotherapeutic agent, probably due to the upregulation of antiapoptotic transcription factor NF-κB.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry

Journal Section

Research Article

Authors

Ecem Kaya Sezginer ^*
0000-0002-8490-6293
Türkiye

Omer Faruk Kırlangıç
0000-0003-0219-3312
Türkiye

Cengiz Karakaya
0000-0002-0399-9150
Türkiye

Sema Ören
0000-0001-7375-2009
Türkiye

Tuğba Fatsa
0000-0003-3759-495X
Türkiye

Taner Özgürtaş
0000-0003-1110-6671
Türkiye

Publication Date

June 28, 2025

Submission Date

October 12, 2022

Acceptance Date

November 25, 2022

Published in Issue

Year 2023 Volume: 27 Number: 1

IZ

https://izlik.org/JA52WY67CD

Cite

RIS / Bibtex

APA

Kaya Sezginer, E., Kırlangıç, O. F., Karakaya, C., Ören, S., Fatsa, T., & Özgürtaş, T. (2025). Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells. Journal of Research in Pharmacy, 27(1), 329-338. https://izlik.org/JA52WY67CD

AMA

1.Kaya Sezginer E, Kırlangıç OF, Karakaya C, Ören S, Fatsa T, Özgürtaş T. Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells. J. Res. Pharm. 2025;27(1):329-338. https://izlik.org/JA52WY67CD

Chicago

Kaya Sezginer, Ecem, Omer Faruk Kırlangıç, Cengiz Karakaya, Sema Ören, Tuğba Fatsa, and Taner Özgürtaş. 2025. “Apoptosis Inducing Effect of Sodium Butyrate and Cisplatin Alone or in Combination on SH-SY5Y Human Neuroblastoma Cells”. Journal of Research in Pharmacy 27 (1): 329-38. https://izlik.org/JA52WY67CD.

EndNote

Kaya Sezginer E, Kırlangıç OF, Karakaya C, Ören S, Fatsa T, Özgürtaş T (June 1, 2025) Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells. Journal of Research in Pharmacy 27 1 329–338.

IEEE

[1]E. Kaya Sezginer, O. F. Kırlangıç, C. Karakaya, S. Ören, T. Fatsa, and T. Özgürtaş, “Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells”, J. Res. Pharm., vol. 27, no. 1, pp. 329–338, June 2025, [Online]. Available: https://izlik.org/JA52WY67CD

ISNAD

Kaya Sezginer, Ecem - Kırlangıç, Omer Faruk - Karakaya, Cengiz - Ören, Sema - Fatsa, Tuğba - Özgürtaş, Taner. “Apoptosis Inducing Effect of Sodium Butyrate and Cisplatin Alone or in Combination on SH-SY5Y Human Neuroblastoma Cells”. Journal of Research in Pharmacy 27/1 (June 1, 2025): 329-338. https://izlik.org/JA52WY67CD.

JAMA

1.Kaya Sezginer E, Kırlangıç OF, Karakaya C, Ören S, Fatsa T, Özgürtaş T. Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells. J. Res. Pharm. 2025;27:329–338.

MLA

Kaya Sezginer, Ecem, et al. “Apoptosis Inducing Effect of Sodium Butyrate and Cisplatin Alone or in Combination on SH-SY5Y Human Neuroblastoma Cells”. Journal of Research in Pharmacy, vol. 27, no. 1, June 2025, pp. 329-38, https://izlik.org/JA52WY67CD.

Vancouver

1.Ecem Kaya Sezginer, Omer Faruk Kırlangıç, Cengiz Karakaya, Sema Ören, Tuğba Fatsa, Taner Özgürtaş. Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(1):329-38. Available from: https://izlik.org/JA52WY67CD