Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations

Sakine Atila Karaca; Büşra Güvercin

doi:10.12991/jrespharm.1695536

Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations

Abstract

Latanoprostene bunod has recently emerged as a promising ocular antihypertensive agent due to its potent ability to lower intraocular pressure and its favorable safety profile observed in clinical trials. In this study, a new, simple, and selective HPLC method was developed for quantifying latanoprostene bunod in pharmaceutical formulations. A Box-Behnken experimental design was employed to optimize the chromatographic parameters, including flow rate, organic solvent, and acid content in the mobile phase. The analysis was performed on an C18 column (4.6 × 50 mm, 3.5 µm), with a mobile phase consisting of water and acetonitrile, both containing 0.01% formic acid (45:55, v/v). Chromatographic conditions were set at a flow rate of 0.6 mL/min, a column temperature of 40 °C, and detection at 260 nm. Method validation, following the ICH Q2(R1) guideline, demonstrated excellent linearity over the concentration range of 0.50–20.00 µg/mL, with LOD and LOQ values of 0.052 µg/mL and 0.157 µg/mL, respectively. Precision and accuracy were confirmed with recovery rates between 100-102% and RSD values below 3%, respectively. The validated method was then successfully applied to the analysis of latanoprostene bunod in a commercially available ophthalmic formulation, confirming its suitability for routine quality control use.

Keywords

Project Number

This study was supported by the Scientific and Technological Research Council of Türkiye (TÜBİTAK), through the 2209-A Research Project Support Program for Undergraduate Students.

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry

Journal Section

Research Article

Authors

Sakine Atila Karaca ^*
0000-0002-9440-3438
Türkiye

Büşra Güvercin This is me
0009-0002-2590-3794
Türkiye

Publication Date

January 11, 2026

Submission Date

May 12, 2025

Acceptance Date

June 10, 2025

Published in Issue

Year 2026 Volume: 30 Number: 1

DOI

https://doi.org/10.12991/jrespharm.1695536

IZ

https://izlik.org/JA63AM64TK

Cite

RIS / Bibtex

APA

Atila Karaca, S., & Güvercin, B. (2026). Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations. Journal of Research in Pharmacy, 30(1), 126-133. https://doi.org/10.12991/jrespharm.1695536

AMA

1.Atila Karaca S, Güvercin B. Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations. J. Res. Pharm. 2026;30(1):126-133. doi:10.12991/jrespharm.1695536

Chicago

Atila Karaca, Sakine, and Büşra Güvercin. 2026. “Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations”. Journal of Research in Pharmacy 30 (1): 126-33. https://doi.org/10.12991/jrespharm.1695536.

EndNote

Atila Karaca S, Güvercin B (January 1, 2026) Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations. Journal of Research in Pharmacy 30 1 126–133.

IEEE

[1]S. Atila Karaca and B. Güvercin, “Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations”, J. Res. Pharm., vol. 30, no. 1, pp. 126–133, Jan. 2026, doi: 10.12991/jrespharm.1695536.

ISNAD

Atila Karaca, Sakine - Güvercin, Büşra. “Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations”. Journal of Research in Pharmacy 30/1 (January 1, 2026): 126-133. https://doi.org/10.12991/jrespharm.1695536.

JAMA

1.Atila Karaca S, Güvercin B. Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations. J. Res. Pharm. 2026;30:126–133.

MLA

Atila Karaca, Sakine, and Büşra Güvercin. “Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations”. Journal of Research in Pharmacy, vol. 30, no. 1, Jan. 2026, pp. 126-33, doi:10.12991/jrespharm.1695536.

Vancouver

1.Sakine Atila Karaca, Büşra Güvercin. Development of an HPLC Method Using Chemometric Approaches for the Determination of Latanoprostene Bunod in Pharmaceutical Preparations. J. Res. Pharm. 2026 Jan. 1;30(1):126-33. doi:10.12991/jrespharm.1695536