Anti-inflammatory effects of Lycium barbarum leaf extracts in lipopolysaccharide-induced RAW 264.7 macrophage cells and isolation of secondary metabolites

Beril Kadıoğlu Yaman; Ozan Şen; Aycan Salman; Hande Sipahi; Norbert Kusz; Judit Hohmann; Hasan Kırmızıbekmez

Anti-inflammatory effects of Lycium barbarum leaf extracts in lipopolysaccharide-induced RAW 264.7 macrophage cells and isolation of secondary metabolites

Abstract

Lycium barbarum possesses many bioactivities such as antidiabetic, antioxidant, antitumor, and immunomodulatory activities. In the present study, the MeOH extract (1 mg/mL) prepared from the leaves of L. barbarum cultivated in Turkey exerted significant anti-inflammatory activity via reducing the release of nitric oxide (NO) in LPSstimulated RAW 264.7 macrophages comparable to positive control indomethacin. Then, the extract was partitioned against CH2Cl2 and EtOAc to yield CH2Cl2, EtOAc and H2O subextracts which were also submitted to the same tests. Among the tested extracts, EtOAc (0.25 and 0.5 mg/mL), and CH2Cl2 (0.25 mg/mL) subextracts showed significant NO production inhibitory activities. The tested extracts also inhibited the production of PGE2 slightly. Successive chromatographic studies on the EtOAc subextract led to the isolation of three secondary metabolites, including chlorogenic acid, hesperidin and rutin as the potential bioactive compounds that are responsible for the in vitro antiinflammatory activity of the EtOAc subextract. The structures were elucidated based on 1D and 2D NMR spectra. Although chlorogenic acid and rutin were previously reported from leaves of L. barbarum, this is the first report of hesperidin from L. barbarum to the best of our knowledge.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy, Pharmaceutical Toxicology, Basic Pharmacology

Journal Section

Research Article

Authors

Beril Kadıoğlu Yaman ^*
Türkiye

Ozan Şen This is me
Türkiye

Aycan Salman This is me
Türkiye

Hande Sipahi
Türkiye

Norbert Kusz This is me
Hungary

Judit Hohmann This is me
Hungary

Hasan Kırmızıbekmez
Türkiye

Publication Date

June 27, 2025

Submission Date

February 22, 2019

Acceptance Date

May 22, 2019

Published in Issue

Year 2019 Volume: 23 Number: 4

IZ

https://izlik.org/JA67HA64CR

Cite

RIS / Bibtex

APA

Kadıoğlu Yaman, B., Şen, O., Salman, A., Sipahi, H., Kusz, N., Hohmann, J., & Kırmızıbekmez, H. (2025). Anti-inflammatory effects of Lycium barbarum leaf extracts in lipopolysaccharide-induced RAW 264.7 macrophage cells and isolation of secondary metabolites. Journal of Research in Pharmacy, 23(4), 740-748. https://izlik.org/JA67HA64CR

AMA

1.Kadıoğlu Yaman B, Şen O, Salman A, et al. Anti-inflammatory effects of Lycium barbarum leaf extracts in lipopolysaccharide-induced RAW 264.7 macrophage cells and isolation of secondary metabolites. J. Res. Pharm. 2025;23(4):740-748. https://izlik.org/JA67HA64CR

Chicago

Kadıoğlu Yaman, Beril, Ozan Şen, Aycan Salman, et al. 2025. “Anti-Inflammatory Effects of Lycium Barbarum Leaf Extracts in Lipopolysaccharide-Induced RAW 264.7 Macrophage Cells and Isolation of Secondary Metabolites”. Journal of Research in Pharmacy 23 (4): 740-48. https://izlik.org/JA67HA64CR.

EndNote

Kadıoğlu Yaman B, Şen O, Salman A, Sipahi H, Kusz N, Hohmann J, Kırmızıbekmez H (June 1, 2025) Anti-inflammatory effects of Lycium barbarum leaf extracts in lipopolysaccharide-induced RAW 264.7 macrophage cells and isolation of secondary metabolites. Journal of Research in Pharmacy 23 4 740–748.

IEEE

[1]B. Kadıoğlu Yaman et al., “Anti-inflammatory effects of Lycium barbarum leaf extracts in lipopolysaccharide-induced RAW 264.7 macrophage cells and isolation of secondary metabolites”, J. Res. Pharm., vol. 23, no. 4, pp. 740–748, June 2025, [Online]. Available: https://izlik.org/JA67HA64CR

ISNAD

Kadıoğlu Yaman, Beril - Şen, Ozan - Salman, Aycan - Sipahi, Hande - Kusz, Norbert - Hohmann, Judit - Kırmızıbekmez, Hasan. “Anti-Inflammatory Effects of Lycium Barbarum Leaf Extracts in Lipopolysaccharide-Induced RAW 264.7 Macrophage Cells and Isolation of Secondary Metabolites”. Journal of Research in Pharmacy 23/4 (June 1, 2025): 740-748. https://izlik.org/JA67HA64CR.

JAMA

1.Kadıoğlu Yaman B, Şen O, Salman A, Sipahi H, Kusz N, Hohmann J, Kırmızıbekmez H. Anti-inflammatory effects of Lycium barbarum leaf extracts in lipopolysaccharide-induced RAW 264.7 macrophage cells and isolation of secondary metabolites. J. Res. Pharm. 2025;23:740–748.

MLA

Kadıoğlu Yaman, Beril, et al. “Anti-Inflammatory Effects of Lycium Barbarum Leaf Extracts in Lipopolysaccharide-Induced RAW 264.7 Macrophage Cells and Isolation of Secondary Metabolites”. Journal of Research in Pharmacy, vol. 23, no. 4, June 2025, pp. 740-8, https://izlik.org/JA67HA64CR.

Vancouver

1.Beril Kadıoğlu Yaman, Ozan Şen, Aycan Salman, Hande Sipahi, Norbert Kusz, Judit Hohmann, Hasan Kırmızıbekmez. Anti-inflammatory effects of Lycium barbarum leaf extracts in lipopolysaccharide-induced RAW 264.7 macrophage cells and isolation of secondary metabolites. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(4):740-8. Available from: https://izlik.org/JA67HA64CR