Comparative study of different oil extraction methods on Moringa oleifera seed oil characteristics, fatty acid composition, antioxidant activity and its authentication

Irnawati Irnawati; Anjar Windarsih; Abdul Rohman; Yamin Yamin; Ruslin Ruslin; Munajah Fajriani; Sherly Intan Ardani; Nurrulhidayah Ahmad Fadzillah; Sri Ambardini; Yuli Perwita Sari

Comparative study of different oil extraction methods on Moringa oleifera seed oil characteristics, fatty acid composition, antioxidant activity and its authentication

Abstract

Moringa oleifera is a native to India and can grow in several regions such as the tropical region. The aims of this study were to evaluate the effect of extraction method on the characteristics, fatty acid methyl ester (FAME) composition, antioxidant activities, and authentication of moringa seed oils (MSO) from Southeast Sulawesi, Indonesia. The Soxhlet and ultrasound-assisted extraction (UAE) methods were used for seed oil extraction. Moringa seeds had an oil yield of 32.24% and 24.88% for the Soxhlet and UAE methods, respectively. The result was found that the quality parameters of MSO, like refractive index, acid value, and peroxide value of MSO obtained from Soxhlet extraction method were were better than those of the Soxhlet method. Saponification value and iodine value method were 142.681±0.353 mg KOH/g oil and 64.6510±0.0640 g I2/100 g oil, where as the UAE method were 181.577±0.214 mg KOH/g oil and 77.3497±0.0639 g I2/100 g oil, respectively. Total saturated fatty acids of MSO extracted using Soxhlet and UAE were 24.78 and 25.32% respectively. The MSO comprises a significant amount of monounsaturated fatty acids (> 74%). Oleic acid was found to be the main unsaturated FAME. The free radical scavenging (ABTS and DPPH methods), and metal chelating agent of MSO obtained from the Soxhlet method were 94.6659 ± 0.0668, 25.536 ± 0.300, and 55.381 ± 0.695%, whereas UAE methods were 93.4210 ± 0.0153, 16.958 ± 0.311, and 68.732 ± 0.0082%, respectively. In addition, discriminant analysis (DA) using middle FTIR was successfully used for authentication of MSO from palm oil (PO) adulteration. The PLS regression using second derivative middle FTIR spectra (3999-650 cm-1) was used to quantification the PO level with high R2 for calibration (0.9993) and validation (0.922) with lower RMSEC value ( 0.922) and RMSEP (1.75%). This study has shown that the MSO obtained from different extraction methods has a good quality oil and has a potential of a new sources of vegetable oil to be produced in Southeast Sulawesi.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry

Journal Section

Research Article

Authors

Irnawati Irnawati ^*
0000-0001-8054-1664
Indonesia

Anjar Windarsih This is me
0000-0002-2235-7575
Indonesia

Abdul Rohman This is me
0000-0002-1141-7093
Indonesia

Yamin Yamin This is me
Indonesia

Ruslin Ruslin This is me
0000-0003-1974-5021
Indonesia

Munajah Fajriani This is me
0009-0004-7222-2355
Indonesia

Sherly Intan Ardani This is me
0009-0008-7705-3449
Indonesia

Nurrulhidayah Ahmad Fadzillah This is me
0000-0001-8524-5100
Malaysia

Sri Ambardini This is me
0000-0003-2916-4570
Indonesia

Yuli Perwita Sari This is me
0009-0003-2680-5392
Indonesia

Publication Date

August 3, 2026

Submission Date

May 20, 2025

Acceptance Date

July 4, 2026

Published in Issue

Year 2026 Volume: 30 Number: 4

IZ

https://izlik.org/JA87DY73CU

Cite

RIS / Bibtex

APA

Irnawati, I., Windarsih, A., Rohman, A., Yamin, Y., Ruslin, R., Fajriani, M., Intan Ardani, S., Ahmad Fadzillah, N., Ambardini, S., & Perwita Sari, Y. (2026). Comparative study of different oil extraction methods on Moringa oleifera seed oil characteristics, fatty acid composition, antioxidant activity and its authentication. Journal of Research in Pharmacy, 30(4), 1104-1114. https://izlik.org/JA87DY73CU

AMA

1.Irnawati I, Windarsih A, Rohman A, et al. Comparative study of different oil extraction methods on Moringa oleifera seed oil characteristics, fatty acid composition, antioxidant activity and its authentication. J. Res. Pharm. 2026;30(4):1104-1114. https://izlik.org/JA87DY73CU

Chicago

Irnawati, Irnawati, Anjar Windarsih, Abdul Rohman, et al. 2026. “Comparative Study of Different Oil Extraction Methods on Moringa Oleifera Seed Oil Characteristics, Fatty Acid Composition, Antioxidant Activity and Its Authentication”. Journal of Research in Pharmacy 30 (4): 1104-14. https://izlik.org/JA87DY73CU.

EndNote

Irnawati I, Windarsih A, Rohman A, Yamin Y, Ruslin R, Fajriani M, Intan Ardani S, Ahmad Fadzillah N, Ambardini S, Perwita Sari Y (August 1, 2026) Comparative study of different oil extraction methods on Moringa oleifera seed oil characteristics, fatty acid composition, antioxidant activity and its authentication. Journal of Research in Pharmacy 30 4 1104–1114.

IEEE

[1]I. Irnawati et al., “Comparative study of different oil extraction methods on Moringa oleifera seed oil characteristics, fatty acid composition, antioxidant activity and its authentication”, J. Res. Pharm., vol. 30, no. 4, pp. 1104–1114, Aug. 2026, [Online]. Available: https://izlik.org/JA87DY73CU

ISNAD

Irnawati, Irnawati - Windarsih, Anjar - Rohman, Abdul - Yamin, Yamin - Ruslin, Ruslin - Fajriani, Munajah - Intan Ardani, Sherly - Ahmad Fadzillah, Nurrulhidayah - Ambardini, Sri - Perwita Sari, Yuli. “Comparative Study of Different Oil Extraction Methods on Moringa Oleifera Seed Oil Characteristics, Fatty Acid Composition, Antioxidant Activity and Its Authentication”. Journal of Research in Pharmacy 30/4 (August 1, 2026): 1104-1114. https://izlik.org/JA87DY73CU.

JAMA

1.Irnawati I, Windarsih A, Rohman A, Yamin Y, Ruslin R, Fajriani M, Intan Ardani S, Ahmad Fadzillah N, Ambardini S, Perwita Sari Y. Comparative study of different oil extraction methods on Moringa oleifera seed oil characteristics, fatty acid composition, antioxidant activity and its authentication. J. Res. Pharm. 2026;30:1104–1114.

MLA

Irnawati, Irnawati, et al. “Comparative Study of Different Oil Extraction Methods on Moringa Oleifera Seed Oil Characteristics, Fatty Acid Composition, Antioxidant Activity and Its Authentication”. Journal of Research in Pharmacy, vol. 30, no. 4, Aug. 2026, pp. 1104-1, https://izlik.org/JA87DY73CU.

Vancouver

1.Irnawati Irnawati, Anjar Windarsih, Abdul Rohman, Yamin Yamin, Ruslin Ruslin, Munajah Fajriani, Sherly Intan Ardani, Nurrulhidayah Ahmad Fadzillah, Sri Ambardini, Yuli Perwita Sari. Comparative study of different oil extraction methods on Moringa oleifera seed oil characteristics, fatty acid composition, antioxidant activity and its authentication. J. Res. Pharm. [Internet]. 2026 Aug. 1;30(4):1104-1. Available from: https://izlik.org/JA87DY73CU