A Novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma

Derya Egeli Yılmaz; Gizem Tırıs; Şerife Evrim Kepekçi Tekkeli

doi:10.12991/jrespharm.1708765

A Novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma

Abstract

This study introduces a combined HPLC and UV detection technique for the quantification of casticin in capsule and human plasma samples. The chromatographic separation was carried out utilizing a C18 column (150 mm × 4.6 mm × 5 μm) at a temperature of 25 ºC. Isocratic elution with a mobile phase comprising 60:40 v/v (methanol-0.05% formic acid) was employed. Flow rate was adjusted 1 mL/min. The analyte was determined at a wavelength of 258 nm, with a retention time of 14.7±0.01 min. The developed method underwent validation according to ICH criteria, covering specificity, linearity, precision, accuracy, detection and quantitation limits, as well as robustness. The linear range was determined to be 10-60 ng/mL for both capsule and spiked plasma specimens. The suggested technique was performed to the analysis of casticin in spiked human plasma and pharmaceutical preparations, yielding a recovery of 106.04% and demonstrating precision through intra-day and inter-day experiments with the highest relative standard deviation (RSD %) value of 4.94. Consequently, the technique was performed to the quantifying of human plasma specimens from in a patient taking medication containing casticin.

Keywords

Supporting Institution

Bezmialem Vakıf University

Project Number

20210219

Ethical Statement

All procedure performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Bezmialem Vakıf University approved by the Clinical Trials Ethic Commitee (No: 12/6).

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry, Pharmacognosy

Journal Section

Research Article

Authors

Derya Egeli Yılmaz ^*
0000-0003-0933-881X
Türkiye

Gizem Tırıs
Türkiye

Şerife Evrim Kepekçi Tekkeli
0000-0002-1871-017X
Türkiye

Publication Date

May 11, 2026

Submission Date

May 29, 2025

Acceptance Date

January 31, 2026

Published in Issue

Year 2026 Volume: 30 Number: 3

DOI

https://doi.org/10.12991/jrespharm.1708765

IZ

https://izlik.org/JA39YR37SD

Cite

RIS / Bibtex

APA

Egeli Yılmaz, D., Tırıs, G., & Kepekçi Tekkeli, Ş. E. (2026). A Novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma. Journal of Research in Pharmacy, 30(3), 826-834. https://doi.org/10.12991/jrespharm.1708765

AMA

1.Egeli Yılmaz D, Tırıs G, Kepekçi Tekkeli ŞE. A Novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma. J. Res. Pharm. 2026;30(3):826-834. doi:10.12991/jrespharm.1708765

Chicago

Egeli Yılmaz, Derya, Gizem Tırıs, and Şerife Evrim Kepekçi Tekkeli. 2026. “A Novel HPLC Technique for the Determination of Casticin in Pharmaceutical Preparations and Human Plasma”. Journal of Research in Pharmacy 30 (3): 826-34. https://doi.org/10.12991/jrespharm.1708765.

EndNote

Egeli Yılmaz D, Tırıs G, Kepekçi Tekkeli ŞE (May 1, 2026) A Novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma. Journal of Research in Pharmacy 30 3 826–834.

IEEE

[1]D. Egeli Yılmaz, G. Tırıs, and Ş. E. Kepekçi Tekkeli, “A Novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma”, J. Res. Pharm., vol. 30, no. 3, pp. 826–834, May 2026, doi: 10.12991/jrespharm.1708765.

ISNAD

Egeli Yılmaz, Derya - Tırıs, Gizem - Kepekçi Tekkeli, Şerife Evrim. “A Novel HPLC Technique for the Determination of Casticin in Pharmaceutical Preparations and Human Plasma”. Journal of Research in Pharmacy 30/3 (May 1, 2026): 826-834. https://doi.org/10.12991/jrespharm.1708765.

JAMA

1.Egeli Yılmaz D, Tırıs G, Kepekçi Tekkeli ŞE. A Novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma. J. Res. Pharm. 2026;30:826–834.

MLA

Egeli Yılmaz, Derya, et al. “A Novel HPLC Technique for the Determination of Casticin in Pharmaceutical Preparations and Human Plasma”. Journal of Research in Pharmacy, vol. 30, no. 3, May 2026, pp. 826-34, doi:10.12991/jrespharm.1708765.

Vancouver

1.Derya Egeli Yılmaz, Gizem Tırıs, Şerife Evrim Kepekçi Tekkeli. A Novel HPLC technique for the determination of casticin in pharmaceutical preparations and human plasma. J. Res. Pharm. 2026 May 1;30(3):826-34. doi:10.12991/jrespharm.1708765