Bioactivity guided fractionation for investigation of antidiabetic activity on the different Origanum species from the Brevifilamentum section

Year 2025, Volume: 29 Issue: 3, 1295 - 1300, 04.06.2025

Başak Tanriverdi Ali Şen , Narin Sadıkoğlu , Leyla Bitiş

https://doi.org/10.12991/jrespharm.1712372

Abstract

The aim of this study was to evaluate antidiabetic activities of Origanum bargyli (OB), Origanum haussknechtii (OH), Origanum rotundifolium (OR) species from Brevifilamentum section of genus Origanum and to investigate their roles in diabetes with the support of their antioxidant effects. Antidiabetic activities on OB, OH, and OR were investigated for the first time in the present study. DPPH and ABTS radicals scavenging, α-glucosidase inhibition methods were performed for antioxidant, antidiabetic activities, respectively. OB (12.70 and 32.62 μg/mL) and OR (10.61 and 32.28 μg/mL) exhibited strong antioxidant activity against DPPH and ABTS radicals with remarkable IC50 values. The amounts of total phenolic and total flavonoid compounds of OB, OH and OR were 67.55, 68.97, 68.93 mg/g and 48.46, 40.54 and 62.28 mg/g, respectively. Whereas OH and OR showed IC50 values of 259 and 404 μg/mL for antidiabetic activity, OB was found to be the most antidiabetic active species, showing more significant inhibitory activity against α-glucosidase enzyme than the standard with an IC50 value of 204.20 μg/ml. Therefore, the OB ethanol extract was fractionated with solvents of different polarity (hexane, chloroform and ethyl acetate, respectively). In the fractions of OB species, chloroform fraction(OBC) showed a much higher inhibitory activity against α-glucosidase enzyme with an IC50 value of 126.50 μg/mL compared to the standard acarbose (261.7 μg/mL) and was found to be the most antidiabetic active fraction. These results suggest that OB species with significant antidiabetic and antioxidant activity may play a good potential role in developing new herbal medicines against diabetes.

Keywords

Origanum bargyli , O. haussknechtii , O. rotundifolium , DPPH/ABTS , α-glucosidase

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