JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES

Burcu Üner; Ahmet Doğan Ergin; Ayça Altay Benetti; Juste Baranauskaıte Ortasöz

JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive and incurable malignancy that is anticipated to be the second leading cause of cancer-related death by 2030. Several signaling pathways involved in growth and proliferation are activated in PDAC. Notwithstanding the progress in pancreatic cancer research over the past decade, effective treatment regimens are lacking. Chemotherapy and radiation therapy are often ineffective [1, 2]. Saponin-derived compounds have proven to be promising in treating many types of cancer, and it is anticipated that they can be used as drugs [3,4]. In this study, mycelial formulations were created based on a DoE perspective with triterpenoid saponins obtained from the marketed product (ABX-Abraxane)[5]. Aesculus hippocastanum L. has used to get saponin. Following characterization studies of micelles (FT-IR, DLS, and TEM), cell-binding and Papp values were analyzed using the human pancreas adenocarcinoma ascites metastasis cell line (AsPC-1). In addition, the levels of proto-oncogenes (KRAS, CTNNB1 (β-catenin), PIK3CA, and AKT) involved in pancreatic carcinogenesis were measured by using ELISA, and the mitochondrial membrane permeability and alterations of oxidative stress levels have been measured by flow cytometry. As a result of the treatment with the control group and micelle formulations on AsPC-1 cells, Annexin-V positive stained areas showed a positive result in PI (late apoptotic). When the apoptotic effect on AsPC-1 cells as a result of the application of micelle formulation was evaluated in flow cytometry at the end of 12 (AsPC-1 72.3% to 47.2%) and 24 (AsPC-1 47.2% to 16.3%) hour treatments, a decrease in cell viability percentage was observed, while an increase in the percentage of necrotic cells was determined. Besides, it was determined that there was a significant increase in ROS levels with micelle formulation compared to the control (13.5 to 67.8).

Keywords

References

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[2] Otte JM, Kiehne K, Schmitz F, Fölsch UR, Herzig KH. C-met protooncogene expression and its regulation by cytokines in the regenerating pancreas and pancreatic cancer cells. Scand J Gastroenterol. 2000; 35: 90-95 [CrossRef].
[3] Xu XH, Li T, Fong CM, Chen X, Chen XJ, Wang YT, Huang MQ, Lu JJ. Saponins from Chinese medicines as anticancer agents. Molecules. 2016;21(10):1326 [CrossRef].
[4] Celep AGS, Yilmaz S, Coruh N. Antioxidant capacity and cytotoxicity of Aesculus hippocastanum on breast cancer MCF-7 cells. J Food Drug Anal. 2012; 20 (3): 17 [CrossRef].
[5] Boateng-Marfo Y, Dong Y, Ng WK, Lin H-S. Artemether-loaded zein nanoparticles: An innovative intravenous dosage form for the management of severe malaria. Int J Mol Sci. 2021; 22(3):1141 [CrossRef].

Details

Primary Language

English

Subjects

Pharmaceutical Delivery Technologies

Journal Section

Conference Paper

Authors

Burcu Üner ^*
0000-0003-4691-0432
United States

Ahmet Doğan Ergin
0000-0002-9387-0085
Türkiye

Ayça Altay Benetti This is me
0000-0001-8072-1606
Singapore

Juste Baranauskaıte Ortasöz
0000-0002-0358-5759
Türkiye

Publication Date

June 28, 2025

Submission Date

February 28, 2023

Acceptance Date

March 14, 2023

Published in Issue

Year 2023 Volume: 27 Number: Current Research Topıcs In Pharmacy: Drug Delivery

IZ

https://izlik.org/JA85TE46UP

Cite

RIS / Bibtex

APA

Üner, B., Ergin, A. D., Altay Benetti, A., & Baranauskaıte Ortasöz, J. (2025). JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES. Journal of Research in Pharmacy, 27(Current Research Topıcs In Pharmacy: Drug Delivery), 17-19. https://izlik.org/JA85TE46UP

AMA

1.Üner B, Ergin AD, Altay Benetti A, Baranauskaıte Ortasöz J. JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES. J. Res. Pharm. 2025;27(Current Research Topıcs In Pharmacy: Drug Delivery):17-19. https://izlik.org/JA85TE46UP

Chicago

Üner, Burcu, Ahmet Doğan Ergin, Ayça Altay Benetti, and Juste Baranauskaıte Ortasöz. 2025. “JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES”. Journal of Research in Pharmacy 27 (Current Research Topıcs In Pharmacy: Drug Delivery): 17-19. https://izlik.org/JA85TE46UP.

EndNote

Üner B, Ergin AD, Altay Benetti A, Baranauskaıte Ortasöz J (July 1, 2025) JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES. Journal of Research in Pharmacy 27 Current Research Topıcs In Pharmacy: Drug Delivery 17–19.

IEEE

[1]B. Üner, A. D. Ergin, A. Altay Benetti, and J. Baranauskaıte Ortasöz, “JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES”, J. Res. Pharm., vol. 27, no. Current Research Topıcs In Pharmacy: Drug Delivery, pp. 17–19, July 2025, [Online]. Available: https://izlik.org/JA85TE46UP

ISNAD

Üner, Burcu - Ergin, Ahmet Doğan - Altay Benetti, Ayça - Baranauskaıte Ortasöz, Juste. “JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES”. Journal of Research in Pharmacy 27/Current Research Topıcs In Pharmacy: Drug Delivery (July 1, 2025): 17-19. https://izlik.org/JA85TE46UP.

JAMA

1.Üner B, Ergin AD, Altay Benetti A, Baranauskaıte Ortasöz J. JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES. J. Res. Pharm. 2025;27:17–19.

MLA

Üner, Burcu, et al. “JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES”. Journal of Research in Pharmacy, vol. 27, no. Current Research Topıcs In Pharmacy: Drug Delivery, July 2025, pp. 17-19, https://izlik.org/JA85TE46UP.

Vancouver

1.Burcu Üner, Ahmet Doğan Ergin, Ayça Altay Benetti, Juste Baranauskaıte Ortasöz. JOURNEY OF THE SAPONIN FROM THE PLANT TO THE FORMULATION FOR BLOCKING TUMOR ACTIVITIES. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(Current Research Topıcs In Pharmacy: Drug Delivery):17-9. Available from: https://izlik.org/JA85TE46UP